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  • 1
    Electronic Resource
    Electronic Resource
    Diversity of free-living prokaryotes from a deep-sea site at the Antarctic Polar Front (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 36 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To contribute to the understanding of deep-sea planktonic communities, we explored the prokaryotic diversity of a 3000 m deep site at the Antarctic Polar Front using molecular methods. Bacterial 16S rDNA-amplified sequences corresponded to the as yet uncultivated groups SAR11, within the α-Proteobacteria, and SAR324, within the δ-Proteobacteria, as well as to the γ-Proteobacteria, Cytophagales, Planctomyces, Gram-positives, and the group of environmental sequences SAR406. Among them, γ-proteobacterial sequences were the most abundant and diverse. Within Archaea, and using six different primer sets for 16S rDNA amplification, only euryarchaeotal sequences were retrieved. Most of them clustered with the Thermoplasma-related marine groups II and III, but some corresponded to a recently described group of marine sequences emerging at the base of haloarchaea. Our data suggest that γ-Proteobacteria and Euryarchaeota may be dominant elements in terms of genetic diversity of the two prokaryotic domains in this deep-sea pelagic area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00840.x
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of an insertion sequence-like element identified in plasmid pCIT264 from Lactococcus lactis subsp. lactis biovar diacetylactis (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 136 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Plasmid pCIT264 from Lactococcus lactis subsp. lactis biovar diacetylactis (L. diacetylactis) contains an insertion sequence (IS)-like element located in the citrate utilization (citQRP) cluster. This 967-nucleotide long element is bounded by 17 bp perfect inverted repeats and contains an open reading frame (ORF1) composed of 296 codons, which could encode a transposase. Expression of the IS from pCIT264 generates two mRNAs of 2900 and 1900 nucleotides. The transcription is driven by the P3 promoter, composed of a — 10 region located at the right end of the IS and of a — 35 region positioned downstream of this element. The IS-like element (IS982) is present in seven copies in the L. diacetylactis genome. The copy present in pCIT264 is highly stable and does not promote rearrangements of the cit cluster. We suggest that the stable maintenance of the IS-like element in pCIT264 could be due to a translational control of the putative transposase by an antisense RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08063.x
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative analysis of gene expression in Streptococcus pneumoniae and Lactococcus lactis (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 122 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The pFL10 plasmid vector for translational fusions was constructed. pFL10 is based in the promiscuous pLS1 replicon and contains the pC194 cat gene deprived of its transcriptional promoter and Shine-Dalgarno (SD) sequence. Three promoters (Pcit, PpolA and PtetL) from Gram-positive bacteria, inserted in pFL10, were tested for their ability to drive transcription in Lactococcus lactis and Streptococcus pneumoniae. These promoters were coupled to the SD sequence of the lactococcal citP gene fused to the cat gene. Determination of the 5′ ends of the three mRNAs by primer extension revealed the same start sites in both bacterial systems. However, it was observed a differential efficiency of promoter utilization by the RNA polymerases from the two hosts. The transcriptional behavior correlates with expression of the cat gene measured by determinations of chloramphenicol acethyltransferase (CAT) activity. Substitution of the SD of citP by that of the T7 φ10 gene rendered a similar decrease of the CAT production in both bacterial systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07182.x
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  • 4
    Electronic Resource
    Electronic Resource
    Citrate transport in Lactococcus lactis biovar diacetylactis: Expression of the plasmid-borne citrate permease P (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 118 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The Lactococcus lactis subsp. lactis biovar diacetylactis citrate permease P is encoded by the citP gene borne by an 8.3-kb Cit+ plasmid (F. Sesma, Gardiol, D., de Ruiz Holgado, A.P. and de Mendoza, D., Appl. Environ. Microbiol. 56 (1990) 2099–2103). By subcloning different DNA fragments from the Cit+ plasmid it was found that citP is the only plasmidic gene required for the transport of citrate. Northern blot analysis revealed that citP is transcribed in two mRNA species with lengths of 2.9 and 1.9 kb. The transcriptional pattern, the total amount of citP mRNAs and the transport of citrate were unaffected by the presence of citrate in the growth media. Our results support the conclusion that transcription of citP as well as citrate transport are not induced by citrate in Lactococcus lactis subsp. lactis biovar diacetylactis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06806.x
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  • 5
    Electronic Resource
    Electronic Resource
    Immunization of Cattle with an Inactivated Polyvalent Vaccine against Anaplasmosis and Babesiosis (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 653 (1992), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1992.tb19634.x
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of Paprika (Capsicum annum) on Color of Spanish-type Sausages During the Resting Stage (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : The effects of 3 particle sizes of the mince and 3 paprika concentrations on color parameters (CIELAB color space) of Spanish-type sausage during the resting stage were studied. The addition of paprika to minced meat reduced lightness. Redness values of minced meat batters with added paprika (30 g/kg) were a result of the paprika they contain, and because the effect of the meat color itself was masked. The contribution of meat yellow components to yellowness values differed with the degree of mincing and increased with coarseness. The effect of paprika on the reflectance spectra of the 3 minces studied was similar and determined the form of the spectrum regardless of the product or processing. Modifications of the color of products containing paprika were due principally to changes which the spice underwent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb09562.x
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  • 7
    Electronic Resource
    Electronic Resource
    Aluminum Content in Foods and Beverages Consumed in the Spanish Diet (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A reliable and rapid method for determining aluminum content in foods and beverages was developed using electrothermal atomization-atomic absorption spectrometry (ETA-AAS) using previous optimization of the time-temperature program for the graphite furnace. The samples were subjected to acid mineralization with HNO3 and V2O5. The technique used slurries for assay in samples of dairy products. The detection limit was 4.0 pg. The mean recovery obtained ranged from 98.5% to 99.0%. The variation coefficient ranged from 3.2% to 5.2%. The results obtained ranged from 1.362 to 6.610 μg/g in seafood, 0.171 to 29.688 μg/g in vegetables, 19.560 to 70.100 μg/g in olive oil, 0.424 to 6.430 μg/g in dairy products and 25.600 to 58.057 μg/g in stimulant drinks and infusions. This study contributes new data on the Al content of a variety of foods and beverages in Spain and such data are important for composition tables. The higher presence corresponded to seafood, vegetables and dairy products. Their contribution to Al dietary intake were estimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2000.tb15980.x
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  • 8
    Electronic Resource
    Electronic Resource
    Enzymatic Production of a Low-Phenylalanine Product from Skim Milk Powder and Casemate (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 56 (1991), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Skim milk powder and commercial casein were used as raw materials. Enzymatic hydrolysis involved a 5-hr treatment with a protease from Aspergillus oryzae followed by papain hydrolysis for 21 hr to liberate 16.5 mg phenylalanine/gram protein. Removal of phenylalanine from the hydrolysate was by physical adsorption onto activated carbon: 3g carbon/g casein removed 92% of the total phenylalanine. After evaporation, the mixture of peptides was mixed with butter oil and other ingredients, homogenized and spray-dried. The product was then formulated with essential nutrients, as well as tryptophan, histidine, tyrosine and methionine which were lost due to nonspecific adsorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1991.tb14610.x
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  • 9
    Electronic Resource
    Electronic Resource
    Macrofilamentous microbial communities in the metal-rich and acidic River Tinto, Spain (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 235 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A novel type of macroscopic microbial community consisting of large dendritic filaments (up to 1.5 m) in a pH 2.0 dam of the River Tinto (South-western Spain) is described. The combined use of 16S rRNA-gene surveys and fluorescent in situ hybridisation (FISH) suggested that γ-proteobacteria and a relative large diversity of α-proteobacteria dominated these structures. β-Proteobacteria, Actinobacteria and Firmicutes were also detected. Whereas acidophilic bacteria of the genera Acidithiobacillus, Leptospirillum and Acidiphilium, and archaea belonging to the Thermoplasmatales dominate mine acid drainage waters and streamers (riverbed filamentous biofilms), none of the lineages identified in this study affiliate to typical acid mine drainage acidophilic bacteria. Bacteria of the Tinto macrofilaments might be heterotrophic, and could be feeding on the organic matter entrapped in the filamentous structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09592.x
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  • 10
    Electronic Resource
    Electronic Resource
    Differentiation of Mediterranean plum pox virus isolates by coat protein analysis (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 43 (1994), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Six isolates of plum pox potyvirus from different Mediterranean countries were compared by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), peptide mapping and Western blotting after improved purification of virions using a protease inhibitor cocktail that reduced coat protein degradation. One isolate (Spanish isolate 3.3 from plum) differed from the others in possessing a smaller coat protein (approximately 34 instead 36 kDa) with a possible deletion in the surface-exposed amino-terminal region. Infectivity of the viruses after proteolysis, assessed using a local lesion host, was significantly reduced. Protease digestion conditions were established to generate a 28 kDa resistant core of the viral coat protein. Such conditions (longer incubation times or an increase in the enzyme concentration) differed from the milder ones reported for other potyviruses. Implications of the results in relation to the production and screening of virus-specific monoclonal antibodies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1994.tb00566.x
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