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  • 1
    Electronic Resource
    Electronic Resource
    Development of a microtitre ELISA to quantify development of Cryptosporidium parvum in vitro (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 128 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract An in situ enzyme-linked immunosorbent assay (ELISA) was developed to evaluate growth of Cryptosporidium parvum in vitro. Ninety-six-well tissue culture microtitre plates were each seeded with 4.0 X 104 human ileocecal adenocarcinoma (HCT-8) cells, then infected with CsCl-purified oocysts 24 h later. The growth medium consisted of RPMI 1640 supplemented with 10% fetal bovine serum, 15 mM HEPES (JV-2-hydroxyethylpiperazine N−2-ethanesulfonic acid), 50 mM glucose, 1 μg ml−1 folic acid, 4 μg ml−1 4-aminobenzoic acid, 2 μg ml−1 pantothenic acid and 35 μg ml−1 ascorbic acid. Incubation conditions were at 37 ° C in a 5% CO2/95% humidified air incubator. Oocysts were allowed to excyst in situ so that sporozoites could infect cells directly. Monolayers were then washed, new medium added, and infected cells re-incubated. Levels of infection were assessed 48 h later using a rat anti-C. parvum polyvalent antiserum directed against purified parasite membranes, followed by a goat anti-rat IgG conjugated to horseradish peroxidase and 3,3′,5,5′-tetramethyl-benzidine as substrate. Using various parasite inoculating doses and incubation times, optimal results were obtained using a 90-min exposure of host cells to 2.5−3.0 × 104 oocysts/well. Evaluation of various concentrations of four anti-microbials (monensin, lasalocid, paromomycin and sulfadimethoxine) in the system resulted in the acquisition of precise dose-response curves for each compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07505.x
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  • 2
    Electronic Resource
    Electronic Resource
    Oxytocin Facilitation of Maternal Behavior in Sheep (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 652 (1992), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1992.tb34348.x
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  • 3
    Electronic Resource
    Electronic Resource
    Correlation of the Avermectin Polyketide Synthase Genes to the Avermectin Structure: Implications for Designing Novel Avermectins (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 721 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb47384.x
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  • 4
    Electronic Resource
    Electronic Resource
    V-GENE Repertoire and Hypermutation of Rheumatoid Factors Produced in Rheumatoid Synovial Inflammation and Immunized Healthy Donors (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 764 (1995), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1995.tb55861.x
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  • 5
    Electronic Resource
    Electronic Resource
    Sampling for Toxic Contaminants in Ground Water (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water monitoring & remediation 6 (1986), S. 0 
    Details
    ISSN: 1745-6592
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: In this paper, we relate recent developments in ground water sampling techniques to the practical application of sampling for toxic contaminants in ground water. We address the choices that must be made in choosing equipment for a particular project by going through a step-by-step procedure for collecting a ground water sample from a typical monitoring well. Ground water sampling topics that are discussed include: choice of equipment for purging and sampling a well, monitoring for purged ground water indicators and quality assurance/quality control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6592.1986.tb01241.x
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  • 6
    Electronic Resource
    Electronic Resource
    Role of Geological and Geophysical Data in Modeling a Southwestern Alluvial Basin (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 22 (1984), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A parameter-estimation technique based on existing hydrological, geophysical, and geological data was developed to approximate transmissivity values for use in a ground-water flow model of the Animas Valley, southwest New Mexico. Complete Bouguer gravity anomaly maps together with seismic-refraction profiles, geologic maps, geologic, geophysical, and drillers' logs, water levels, and pumping-test data provide insight into the transmissivity of bolson deposits throughout the basin.The transmissivity distribution was primarily based on reported pumping and specific-capacity tests in conjunction with complete Bouguer gravity anomaly maps and well log data. Reported transmissivity values were characterized by gravity values and well log data. In grid blocks lacking pumping and specific-capacity tests, transmissivity values were assigned based on the relationship of gravity values and well log data within the grid block to gravity values and well log data within other grid blocks for which transmissivity values are available.A two-dimensional, finite-difference, ground-water flow computer code was used to evaluate the effectiveness of the parameter-estimation technique. Although the trial-and-error method of calibration was employed, the actual computer time necessary for model calibration was minimal. The conceptually straightforward approach for parameter estimation utilizing existing hydrological, geophysical, and geological data provides realistic parameter estimates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1984.tb01440.x
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  • 7
    Electronic Resource
    Electronic Resource
    A systematic survey for thermophilic fermentative bacteria and archaea in high temperature petroleum reservoirs (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 21 (1996), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Production waters from 36 high temperature petroleum reservoirs were examined for the presence of thermophilic, fermentative microorganisms. The direct supplementation of production waters with glucose and either yeast extract, peptone, tryptone or casamino acid resulted in the isolation of thermophilic, fermentative microorganisms from 47% of the petroleum reservoirs examined. Three distinctive morphological groups were isolated from the production waters of petroleum reservoirs with depths ranging from 396–3048 metres, temperatures ranging from 21–130°C, salinities ranging from 2.8–128 gl−1 and pHs ranging from 6.0–8.5. Group 1 were pleomorphic rod-shaped bacteria, Group 2 were sheathed rod-shaped bacteria, and Group 3 were coccoid archaea. Partial characterisation of strains from one seawater-flooded petroleum reservoir and three non-waterflooded petroleum reservoirs tentatively identified some strains in Group 1 as members of the genera Thermoanaerobacter and Thermoanaerobacterium, Group 2 as members of the Thermotogales order, and Group 3 as members of the genus Thermococcus. Production water salinity determined the type of microorganisms that were isolated. Group 1 organisms were found primarily in petroleum reservoirs with salinities less than 30 g/l, while Group 2 and 3 organisms were found to dominate in more saline reservoirs. The successful isolation of thermophilic, fermentative microorganisms from petroleum reservoirs decreased significantly with increasing salinity and temperature. These findings support the existence of a deep biosphere where fermentative microorganisms are widespread.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1996.tb00332.x
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  • 8
    Electronic Resource
    Electronic Resource
    Efficacy of select antivirals against Cryptosporidium parvum in vitro (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 168 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cryptosporidium parvum is an intestinal pathogen associated with diarrheal disease in both humans and animals. Currently, no effective therapy exists to eliminate the parasite in the absence of a healthy, intact immune system. We used an in situ, enzyme-linked immunosorbent assay (ELISA) as a primary screen to examine the effects of 13 antivirals on the development of C. parvum in human ileocecal adenocarcinoma (HCT-8) cells in vitro. Six of the compounds displayed some efficacy, and dose-response curves and toxicity assays were generated for each of the six compounds. All six were nucleoside analogs, and five of the six were structurally related. These results suggest one potential strategy for therapeutic intervention of C. parvum may be the use and development of certain types of nucleoside analogs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13255.x
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  • 9
    Electronic Resource
    Electronic Resource
    Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 21 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The transposase protein encoded by the insertion element IS 903 belongs to an unusual class of DNA-binding proteins, termed cis-acting proteins, that act preferentially at their site of synthesis. Previous work had led us to propose that instability of the IS 903 transposase was a major determinant of its cis preference. Here we describe the isolation of two classes of mutations within the transposase gene that increased action in trans. One class specifically increased trans action without increasing the level of transposition when the mutant gene was located in cis to the transposon. In particular, a threonine-to-proline substitution at amino acid 25 (T25P) reduced cis preference about 60-fold. The half-life of this mutant transposase was significantly longer than that of the wild-type transposase, confirming the critical role of protein instability. The second, larger, class of mutations increased the level of transposition both in trans and in cis. The behaviour and location of these mutations were consistent with an increase in gene expression by improving translational initiation. Several of these mutations exerted a disproportionate effect on the action of transposase in trans, implying that translation efficiency may affect more than just the amount of transposase made. Our results indicate that cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation initiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1996.tb02587.x
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