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  • 1
    Electronic Resource
    Electronic Resource
    Transcriptional regulation of the Trichoderma longibrachiatum egl1 gene (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 122 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Transcription of the Trichoderma longibrachiatum egl1 gene is induced in the presence of lactose and β-methylglucoside and repressed by glucose. A DNA fragment containing 722 bp upstream of the ATG codon has been sequenced. The gene has two major transcription start points (20 and 24 nucleotides upstream from the ATG codon) and several transcription termination points (located in a region around 130 nt downstream of the stop codon). Two 6-mer sequences (5′-CTGGAG-3′) separated by 16 bp are present in the egl1 gene promoter. These sequences match the Aspergillus nidulans consensus CreA binding site and might be implicated in carbon catabolite repression of egl1 transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07184.x
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  • 2
    Electronic Resource
    Electronic Resource
    EFFECTS OF THE HEATING MEDIUM pH ON HEAT RESISTANCE OF BACILLUS CEREUS SPORES (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 18 (1998), S. 0 
    Details
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of the pH of the heating medium (which included several foods and buffers) on the thermal resistance (D and z-values) of spores of three Bacillus cereus strains was studied. Acidification from pH 7.0 to 4.0 produced a 5-fold decrease in D-values. Plots of log D vs pH gave straight lines, which made it possible to develop an equation to approximately predict the changes in heat sensitivity of B. cereus spores which occurred with changing pH. z-Values for two of the strains studied were not affected by acidification. On the other hand, with the strain ATCC 9818, a clear and statistically significant increase in z-value was observed as the pH decreased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4565.1998.tb00199.x
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  • 3
    Electronic Resource
    Electronic Resource
    Modified xylE and xylTE reporter genes for use in Streptomyces: analysis of the effect of xylT (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 196 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3-dioxygenase activities are significantly higher when xylT is present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10569.x
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  • 4
    Electronic Resource
    Electronic Resource
    Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 245 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Aspergillus species included in section Nigri are common in plant products and processed food, such as grapes, cereals, coffee and derivatives, particularly in warm and tropical climates. Two of these species, A. carbonarius and A. niger, are known to produce ochratoxin A (OTA), a potent nephrotoxin and carcinogenic to human (group 2B). Recognition of the several species of this section is difficult and requires considerable expertise using conventional methods based on morphological features. In this work we describe rapid, sensitive and robust assays based on the PCR technique to discriminate the main species included in section Nigri: A. japonicus, A. heteromorphus, A. ellipticus and the two morphologically indistinguishable species of the A. niger aggregate: A. niger and A. tubingensis. The species-specific primers have been designed on the basis of ITS (internal transcribed spacers of rDNA units) sequence comparisons obtained from several Aspergillus strains and have been tested in a number of strains from different origins and hosts. These PCR assays, based on multi-copy sequences, are highly sensitive and specific and represent a good tool for an early detection of OTA-producing Aspergillus species in order to prevent OTA from entering the food chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.03.023
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  • 5
    Electronic Resource
    Electronic Resource
    Time-course of the phosphinothricin effect on gas exchange and nitrate reduction in Medicago sativa (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The long-time effect of phosphinothricin (PPT) on gas exchange and nitrate metabolism in intact plants of lucerne (Medicago sativa L. cv. Aragón) was investigated. Photosynthetic CO2 uptake, stomatal conductance, and transpiration were measured with an Infra-Red Gas Analyzer (IRGA). Under photorespiratory conditions, CO2 uptake continuously decreased after PPT treatment. The decrease of photosynthesis led to an increase in the internal CO2 concentration, which in turn caused stomatal closure and a reduction of transpiration rate. Nitrate reduction from plants sprayed with PPT was assayed both in vitro and in vivo. In vivo nitrate reductase was measured with and without nitrate in the infiltration medium. Both types of nitrate reductase assays indicated that the enzyme was inhibited in plants treated with PPT; however, the enzyme appeared more affected when the in vivo assay was used than when the one in vitro was applied. The nitrate reduction was pronouncedly affected after 24 h of PPT treatment, when glutamine synthetase (GS, EC 6.3.1.2.) activity and gas exchange were inhibited by more than 60%. The data suggest that the inhibition of GS leads to inhibition of photosynthesis, which, in turn, means lack of NADPH and nitrate, the substrates for nitrate reductase. The inhibition of GS also leads to a high ammonia level, which will produce a secondary inhibition of nitrate reductase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1993.tb05295.x
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  • 6
    Electronic Resource
    Electronic Resource
    THERMAL RESISTANCE OF BACILLUS CEREUS SPORES AS AFFECTED BY ADDITIVES IN THE RECOVERY MEDIUM (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 17 (1997), S. 0 
    Details
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of the addition of starch, glucose, sodium chloride, sodium citrate, monopotassium phosphate and disodium phosphate to the recovery medium on apparent heat resistance of Bacillus cereus spores (ATCC 4342, 7004 and 9818) were investigated. Sodium citrate, monopotassium and disodium phosphate at concentrations of 0.1% were effective inhibitory agents for heat injured B. cereus spores especially for strain 9818, although only monopotassium and disodium phosphate caused a significant reduction (p 〈 0.05) in D-values obtained for strain 9818. Sodium chloride also had a marked effect on the recovery of heat injured spores. Concentration as low as 0.5% caused a significant reduction in the recovery rates for strains 9818 and 7004. In all cases, increasing the salt levels from 0.5 to 4% resulted in a progressive decrease in spore recovery. D-values gradually decreased as the salt content increased, although the concentrations which produced statistically significant differences (p 〈 0.05) varied among strains. The addition of starch at 0.1% resulted in a significant increase in the counts for strains 9818 and 7004. In contrast, glucose (0.1%), did not significantly modify the counts obtained Neither of these compounds affected decimal reduction times. No statistical significance (p〉0.05) differences were detected among z-values for the spores of the three strains recovered in the presence of different additives assayed. z-Values ranged from 6.67 to 8.32, with a mean value of 7.56 ± 0.46C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4565.1997.tb00171.x
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  • 7
    Electronic Resource
    Electronic Resource
    3, 4-Dimethylpyrazole phosphate reduces nitrous oxide emissions from grassland after slurry application (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 21 (2005), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. A field study was conducted to assess the effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP), applied at a rate of 1 kg ha−1, on nitrous oxide (N2O) emissions, forage production and N extraction from a grassland soil after cattle slurry applications in autumn and spring. Nitrous oxide emissions were measured daily or weekly using the closed chamber technique. DMPP efficiency after slurry application was lower in spring (16.7 °C mean soil temperature) than in autumn (11.4 °C mean soil temperature). Thus, DMPP was able to maintain soil mineral N in the ammonium form for 22 days and reduce cumulative N2O emissions by 69% in autumn, while in spring its effect on soil mineral N lasted for 7–14 days, reducing cumulative N2O losses by 48%. Furthermore, application of DMPP after slurry did not decrease biomass yield or N uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2005.tb00106.x
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  • 8
    Electronic Resource
    Electronic Resource
    Mitigation of N2O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 18 (2002), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Nitrous oxide (N2O) is involved in both ozone destruction and global warming. In agricultural soils it is produced by nitrification and denitrification mainly after fertilization. Nitrification inhibitors have been proposed as one of the management tools for the reduction of the potential hazards of fertilizer-derived N2O. Addition of nitrification inhibitors to fertilizers maintains soil N in ammonium form, thereby gaseous N losses by nitrification and denitrification are less likely to occur and there is increased N utilization by the sward. We present a study aimed to evaluate the effectiveness of the nitrification inhibitor dicyandiamide (DCD) and of the slurry additive Actilith F2 on N2O emissions following application of calcium ammonium nitrate or cattle slurry to a mixed clover/ryegrass sward in the Basque Country. The results indicate that large differences in N2O emission occur depending on fertilizer type and the presence or absence of a nitrification inhibitor. There is considerable scope for immediate reduction of emissions by applying DCD with calcium ammonium nitrate or cattle slurry. DCD, applied at 25 kg ha–1, reduced the amount of N lost as N2O by 60% and 42% when applied with cattle slurry and calcium ammonium nitrate, respectively. Actilith F2 did not reduce N2O emissions and it produced a long lasting mineralization of previously immobilized added N.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2002.tb00231.x
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  • 9
    Electronic Resource
    Electronic Resource
    Nitrous oxide emissions from grassland in an intensive dairy farm in the Basque Country of Spain (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 17 (2001), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Intensively managed grasslands are potentially a large source of N2O in the North Coast of Spain because of the large N input, the wet soil conditions and mild temperatures. To quantify the effect of fertilizer type and management practices carried out by farmers in this area, field N2O losses were measured over a year using the closed chamber technique. Plots received two types of fertilizer: cattle slurry (536 kg N ha–1) and calcium ammonium nitrate (140 kg N ha–1). N2O losses were less in the slurry treatment than after mineral fertilizer. This was probably due to high, short-lived peaks of N2O encountered immediately following mineral N addition. In contrast, the seasonal distribution of N2O losses from the slurry amended plot was more uniform over the year. The greater N2O losses in the mineral treatment might have been enhanced by the combined effect of mineral fertilizer and past organic residues present from previous organic amendments. Weak relationships were found between N2O emission rates and soil nitrate, soil ammonium, soil water content and temperature. Better relationships were obtained in the mineral treatment than in the slurry plots, because of the wider range in soil mineral N. Water filled pore space (WFPS) was a key factor controlling N2O emissions. In the 〉 90% WFPS range no relationships were found. The best regressions were found for the mineral treatment in the 40–65% WFPS range, 49% of the variance being explained by soil nitrate and ammonium content. In the 65–90% WFPS range, 43% of the variance was explained by nitrate only, but the inclusion of soil ammonium did not improve the model as it did in the 40–65% WFPS range. This fact indicates that nitrification is likely to be an important process involved in N2O emissions at the 40–65% WFPS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2001.tb00017.x
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  • 10
    Electronic Resource
    Electronic Resource
    Changes in Antigenicity of Trypanosoma lewisi During the Course of Infection in Rats (1966)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 13 (1966), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. In rats infected with T. lewisi, 2 morphologically distinct forms of the trypanosome occur. By means of the immunodiffusion technic several antigenic differences between the 2 forms were observed. The time course in the antigenic and morphologic changes is somewhat different. The antigenic change from young to adult forms is gradual. It starts 12 days after infection and is complete in 14 days, 3 to 5 days after the morphologic appearance of the trypanosomes is that of adult forms. In addition, there appear to be 2 separate stages of young forms when they are analyzed for antigenic composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1966.tb01974.x
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