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A site-specific endonuclease activity in Halobacterium halobium (1986)

Schinzel, Reinhard ; Burger, Klaus J.

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 37 (1986), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract In Halobacterium halobium and some related strains, a site-specific endonuclease activity was found. This activity requires 3 M NaCl and 5–10 mM Mg2+ ions for function. The 3 cleavage sites in plasmid pBR322 were mapped, but no homology between these sites was found. H. halobium DNA is resistant to cleavage, which may be due to a modification of the DNA. The behaviour of the endonuclease can be explained by the presence of a Type I or Type III-like restriction-modification system.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01818.x

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