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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 625 (1991), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 628 (1991), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We have used Southern hybridization analysis to characterize the extent of fim homology in recognized type 1 fimbriae mutants of Escherichia coli K12, including strains HB101, P678-54, and VL584. We have found extensive homology in strain HB101, and confirm that P678-54 lacks the majority of fim DNA. Strain VL584 contains a deletion of the entire fim region. We have used a new allelic exchange procedure to generate novel fim deletion derivatives of strains MG1655, MM294, and YMC9. To increase the utility of the new deletion strains we also isolated recA derivatives of each mutant. These strains facilitate the isolation, characterization, and manipulation of cloned fimbriae genes from diverse sources.
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  • 4
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Uropathogenic Escherichia coli frequently express P-pilus adhesins that recognize Galα(1–4)Gal-containing glycoconjugates. The P-pilus adhesin of the, E. coli isolate J96 is encoded by the pap gene cluster and has been shown to agglutinate P1-erythrocytes. We now describe a novel gene cluster from J96, prs, which is responsible for the agglutination of sheep erythrocytes. The structurally related gene clusters both expressed pili exhibiting the F13 antigen. Analysis of mutants of cloned prs sequences, together with trans-complementation of pap and prs genes, identified the sheep-specific adhesin as the 37-kD PrsG protein. The prsG gene occupies the equivalent position in prs as occupied by papG, which specifies the Galeα(1–4)Gal-specific adhesion of pap. PrsG was shown to be structurally distinct from PapG since PapG-specific antiserum did not cross-react with PrsG. Using a solid phase glycolipid receptor binding assay, PrsG was found to specify preferential binding to the Forssman antigen, a major constituent of sheep erythrocyte membranes. The binding epitope was identified as the GaINAcα(1–3)GaINAc moiety. This is the first direct evidence that serologically identical pili may present antigenically distinct adhesins, each capable of binding to a specific receptor.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 44 (1994), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The ultrastructure of the intestinal epithelium has been studied in three species of freshwater fishes, differing from one another in the type of feeding. The pike (a typical predator), the burbot (a predator-facultative benthophage), and the bream (a typical benthophage) were examined. The differences in the condition of epithelial ceils and peculiarities in the structure of the brush border of enterocytes in fishes have been revealed in winter periods. The role of the brush border of enterocytes in the increase of the digestive and transport surface of the intestine in fish is discussed.
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  • 6
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Several hundred recently-metamorphosed Petromyzon marinus were caught during heavy freshwater discharge in the River Severn on the night of 30 November/1 December 1988. The total lengths of a subsample of 42 males and 82 females ranged from 155 to 218 mm, with a mean of 182 mm in both sexes. The buccal funnel, eyes, fins and the teeth on the suctorial disc and tongue-like piston were very well-developed. When placed in full-strength sea water, the lampreys fed on bass (Dicentrarchus labrax), leaving prominent scars on these hosts. The morphology, body size and time of capture of the River Severn sea lampreys provide very strong circumstantial evidence that these lampreys had been feeding for some weeks. Since they were caught when moving downstream and were capable of feeding in sea water, they were presumably about to begin their parasitic marine life.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 19 (1981), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Annual rings on the first dorsal spine were used to determine the age and growth of the catfish, Chrysichthys nigrodigitatus (Lacépède), in the Lagos Lagoon, Nigeria. From back-calculations, it was found that the mean total length ofthe species when the first annulus was formed was 18.5 cm, 32.6 cm for second annulus, 43.5 cm for the third annulus and 53.9 cm for the fourth annulus. It was shown that this species in the Lagos Lagoon attained four years of age and that the fastest growth in length was achieved during the first year of life. Thereafter, increase in length gradually decreased with age. The relationship between the body length and dorsal spine radius was shown to be linear and highly correlated.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sorghum, Sorghum bicolor L. Moench, is grown mostly in semi-arid climates where unpredictable drought stress constitutes a major production constraint. To investigate hybrid performance at different levels of drought stress, 12 single-cross hybrids of grain sorghum and their 24 parent lines were grown in eight site-season combinations in a semi-arid area of Kenya. In addition, a subset of 20 genotypes was evaluated at the seedling stage under polyethylene glycol (PEG)-induced drought stress. Environmental means for grain yield ranged from 47 to 584 g/m2reflecting the following situations: two non-stress, one moderate pre-flowering, four moderate terminal and one extreme drought stress. Mean hybrid superiority over mid-parent values was 54% for grain yield and 35% for above-ground biomass. Across environments, hybrids out-yielded two local varieties by 12%. Differences in yield potential contributed to grain yield differences in all stress environments. Early anthesis was most important for specific adaptation to extreme drought. Field performance was not related to growth reduction and osmotic adjustment under PEG-induced drought stress. In conclusion, exploitation of hybrid vigour could improve the productivity of sorghum in semi-arid areas.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 10 (1987), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The lignotuberous mallee Eucalyptus behriana F. Muell, had much lower predawn leaf water potentials (not higher than – 1.2MPa) than other eucalypts (as high as – 0.2MPa), even after extended rain. This led to the expectation that the lignotuber of E. behriana might have specific hydraulic characteristics. Keeping the soil around partially defoliated mallces for several days underwater did not raise the water status above the maximum leaf water potential observed under natural conditions. Digging a plant out and placing its roots in water after removal of the soil rapidly increased the water status to a level consistant with other eucalypts. This indicated that the major impedance to water uptake was a component of the soil rather than in the roots or in the lignotuber.Some of the individual mallces had only two major stems or branches. One stem or branch was kept covered throughout the experiments to prevent transpiration. The other stem was subjected to a variety of different conditions in order to modify water loss from it. The transpiring branch affected the water status of the non-transpiring plant parts. Hydraulic resistances in the shoot and root/lignotuber were determined from differences in the leaf water potential of covered and uncovered branches, at high water flow rates through the plant. Resistances in branches, including the liquid phase component of the leaf, were significantly larger than in root or lignotuber.The total plant hydraulic resistance of E. behriana was similar to that of other eucalypts, such as E. pauciflora Sieb. ex Spreng. or E. delegatensis R. T. Bak., even though its growth form was different and its natural leaf water potentials were much lower. An osmotic adjustment at the leaf level was observed in the mallee, keeping its bulk leaf turgor in the same range as compared to the other eucalypt species.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 21 (1968), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The changes in the amount and the composition of ribosomes in excised barley leaves floated on water or on 10 mg/l kinetin solution in the dark were examined.The rapid loss of polyribosomes and ribosomes in leaves floated on water was greatly retarded by kinetin. The ribosomes-polyribosomes which originally contained 49 per cent protein showed substantial decline in protein content in leaves floated on water but only slight decline in leaves floated on kinetin solution. It is suggested that kinetin by stimulating RNA synthesis and by suppressing the activities of rihonuclease and peplidase may preserve the ribosomes in excised leaves.
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