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  • 1
    Electronic Resource
    Electronic Resource
    Plasmid analysis in pink facultative methylotrophic bacteria using a modified acetone-alkaline hydrolysis method (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 60 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Routine screening of indigenous and recombinant plasmids in pink facultative methylotrophic bacteria has been difficult, time-consuming, and yields variable results. We report a modified alkaline hydrolysis method for rapid plasmid isolation from these organisms that reproducibly results in good yields of closed circular plasmid DNA which can be readily digested with restriction enzymes. This method greatly facilitates direct screening of indigenous and introduced recombinant plasmids in the methylotrophic host strain. We have confirmed earlier findings that the original NCIB wild-type strain of Methylobacterium sp. strain AM1 (NCIB 9133) contains three cryptic plasmids. However, sizing of these plasmids by comparison to standards and by restriction fragment analysis suggests that they are larger than previously reported. We have designated these plasmids pAM1-1 (65 kb), pAM1-2 (40 kb) and pAM1-3 (33 kb). We have also shown that a rifamycin-resistant strain of Methylobacterium sp. strain AM1 used routinely in our laboratory lacks pAM1-2, although no phenotype has been associated with its loss. Finally, we have shoen that another pink facultative methylotroph, Methylobacterium isolate (#YK1), contains three cryptic plasmids of approximately 43, 37 and 22 kb, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03431.x
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  • 2
    Electronic Resource
    Electronic Resource
    Conjugal transfer of a small plasmid from Bacillus subtilis to Clostridium acetobutylicum by cointegrate formation with plasmid pAMβ1 (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 42 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Plasmid pAMβ1 is transferred at low efficiency in filter matings between Bacillus subtilis and Clostridium acetobutylicum. We have constructed a small non-conjugative plasmid, pOD1 (7.2 kbp; ApR, CmR, EmR) that becomes established in pAMβ1-containing strains of B. subtilis in the form of a cointegrate (pAMβ1::pOD1). The cointegrate plasmid is transferred to, and maintained in C. acetobutylicum, with an efficiency similar to that of pAMβ1. We have also constructed derivatives of plasmid pOD1, with single sites for several commonly used restriction endonucleases. They can be used to transfer foreign genes to C. acetobutylicum via B. subtilis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02056.x
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  • 3
    Electronic Resource
    Electronic Resource
    Conjugal transfer of plasmid pAMβ1 from Streptococcus lactis and Bacillus subtilis to Clostridium acetobutylicum (1985)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 27 (1985), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A filter mating procedure has been used to transfer the plasmid pAMβ;1 from Streptococcus lactis to Clostridium acetobutylicum at high efficiency. Once established in this latter host the plasmid can be transferred, with lower efficiency to other strains of C. acetobutylicum or back to S. lactis. A procedure has also been developed for the co-cultivation of Bacillus subtilis and C. acetobutylicum and the transfer of pAMβ;1 between these organisms has been demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1985.tb01652.x
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  • 4
    Electronic Resource
    Electronic Resource
    Nutrient fluxes in runoff on reseeded blanket bog, limed and fertilized with urea, phosphorus and potassium (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 10 (1994), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Studies have been made of the effects of 15 g N/m2 as urea in two dressings during April and June on annual nutrient fluxes in runoff from reseeded blanket bog also receiving annually 6 g P/m2 as granular superphosphate and 6 kg K/m2 as potassium chloride. Urea applications increased significantly (P 〈 0.05) the mean annual ammonium-N flux from 17 mg/m2 for the P + K plots to 245 mg/m2 for the N + P + K plots. Annual fluxes of total P, K and Ca were also increased (P 〈 0.05) by the addition of urea. This was attributed to the effects of increased acidity around grass roots following N uptake as ammonium-N. In contrast, nitrate-N was removed from rainwater throughout the year and concentrations in runoff were at the limit of detection (〈 0.01 mg/1) on many occasions. Concentrations of organic-N in runoff exceeded those of ammonium-N, but were not significantly changed by fertilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.1994.tb00482.x
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on the Macronucleus of Paramecium aurelia. II. Development of Macronuclear Anlagen (1964)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 11 (1964), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The development of the macronuclear Anlagen of Paramecium aurelia was studied by means of electron, light and ultra-violet microscopy of timed stages following conjugation. In the youngest Anlagen, no differentiated structures could be made out, and staining reactions gave little or no indications of the presence of DNA or RNA. As development proceeds, a number of conspicuous “sponge-like” RNA-containing bodies surrounded by a “matrix” containing DNA can be seen. Eventually these RNA bodies develop DNA centres and apparently disintegrate, yielding the “large bodies” characteristic of the mature macronucleus, and the “small bodies” then also appear. The relation of these observations to interpretations of the structural elements in the macronucleus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1964.tb01786.x
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on the Macronucleus of Paramecium aurelia. I. (with a note on Ultra-violet Micrography). (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. This is a description of structures seen in EM preparations of the macronucleus of Paramecium aurelia, using both starved and growing organisms, the latter at timed stages after fission. The effects of treatment with RNA-ase, DNA-ase and of the “silver-Feulgen” reaction are described, and comparisons made between structures in the macronucleus and the micronucleus. The appearance of macronuclei in thin (2 μ) paraffin sections, stained by the Feulgen, azure A and pyroninmethyl green methods, and upon examination by ultra-violet micrography, is also considered. From these observations it is concluded that the macronucleus contains a large number of structures, 0.5 μ in diameter, consisting of an outer RNA-containing region and central elements containing DNA. These bodies, which are usually thought to be “nucleoli” by other workers, are now considered to be the most likely candidates for the genetic “sub-nuclei” which have been postulated from genetic work.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02593.x
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  • 7
    Electronic Resource
    Electronic Resource
    Salmonella typhimurium metC operator-constitutive mutations (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 60 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We used an Escherichia coli lac deletion strain lysogenized with a metC-lacZ fusion phage (λClac) to select operator-constitutive mutations in the Salmonella typhimurium metC gene control region. The mutations were located in a region containing 2 tandemly repeated 8 bp palindromes previously proposed to be the MetJ repressor binding site. Lysogens carrying λClac mutant phage exhibit high β-galactosidase levels that are only partially repressible by methionine. The results suggest that the mutations disrupt the methionine control system mediated by the metJ gene product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03433.x
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  • 8
    Electronic Resource
    Electronic Resource
    Phenotypic and genotypic differences between certain strains of Clostridium acetobutylicum (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 125 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract It has become evident that several of the strains of Clostridium acetobutylicum that have been employed in physiological studies of the acetone-butanol fermentation, are heterogeneous. Studies of the phenotypic and genotypic characteristics of several of these strains (involving inter alia both pyrolysis mass spectrometry and 16S rRNA sequence determinations) demonstrated that the type strain obtained from ATCC was not identical with that supplied by NCIMB, and that NCIMB 8052T is in fact Clostridium beijerinckii. We therefore suggest that the name Clostridium acetobutylicum should be restricted to those strains that are genetically closely related to ATCC 824T (which include strains DSM 792 and DSM 1731 but not strain P262).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07358.x
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  • 9
    Electronic Resource
    Electronic Resource
    Summer diel activity and movement of adult brown trout in high-elevation streams in Wyoming, U.S.A. (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 54 (1999), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The variation in diel activity patterns was determined by implanting transmitters in adult brown trout of various sizes in two montane streams in two summers in the central Rocky Mountains, U.S.A. More brown trout tended to be active, and to move greater distances, each hour during twilight and night than during the day. Mean hourly light intensity was significantly negatively correlated with the proportion of fish active and with the distance moved. Maximum water temperature was not correlated with any measure of diel activity. Brown trout displayed fidelity to diurnal positions within a given diel cycle. Overall, fish were active an average of 11 h, had an average home range of 41 m, and moved an average of 121 m over the diel cycle. Fish length and distance moved were significantly positively correlated. Water temperature, predator avoidance, and foraging tactics may influence these patterns of diel behaviour in adult brown trout in U.S. streams.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1999.tb00621.x
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  • 10
    Electronic Resource
    Electronic Resource
    Pseudo-allelic relationship between non-homologous genes concerned with biosynthesis of polyglycerol phosphate and polyglycerol phosphate teichoic acids in Bacillus subtilis strains 168 and W23 (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A 60 kbp region of the Bacillus subtilis chromosome encompassing the genes concerned with teichoic acid biosynthesis has been subjected to physical analysis. No homo logy was detected by Southern hybridization between DNA segments encoding the tag genes of strain 168, concerned with polyglycerol phosphate (poly(groP)) biosynthesis, and the tar genes of strain W23, concerned with polyribitol phosphate (poly-(rboP)) biosynthesis. Analysis of 168/W23 interstrain hybrids that incorporate poly(rboP) instead of poly-(groP) into their cell walls revealed that, in every case, Integral substitution of the W23 tar genes for the 168 tag genes had occurred. Interstrain hybrids of the‘W23-like’type have inherited larger segments of W23 DNA than interstrain hybrids of the‘mixed’type. The tag and tar genes are located at equivalent positions on the chromosomes of strains 168 and W23, behaving, in genetic crosses, like an allelic pair. They provide the first example of a pseudo-allelic relationship between non-homologous genes in B. subtilis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00166.x
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