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  • Articles  (73)
  • Springer  (68)
  • Blackwell Publishing Ltd  (5)
  • 1980-1984  (57)
  • 1905-1909  (16)
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  • Articles  (73)
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  • 1
    Electronic Resource
    Electronic Resource
    Preparation of a human lung purified plasma membrane fraction: Confirmation by enzyme markers, electron microscopy, and histamine H1 receptor binding (1984)
    Springer
    The journal of membrane biology 79 (1984), S. 33-39 
    Details
    ISSN: 1432-1424
    Keywords: plasma membrane ; lung ; histamine receptors ; 5′ nucleotidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A simple and rapid method of isolating plasma membranes from human peripheral lung tissue is described. The method involves homogenization of tissue in 0.25m sucrose-buffered medium followed by differential and sucrose density gradient centrifugation. Enzymatic and morphological characterization of the plasma membrane fraction revealed minimal contamination by nonplasma membrane fragments. The isolated plasma membranes showed an 18-fold purification of 5′-nucleotidase activity compared to the original homogenate. Electronmicroscopic studies of the plasma membrane fraction revealed the presence of small membrane vesicles having a trilaminar membrane structure. To further examine the purity of the plasma membrane preparation, the binding of the H1 receptor antagonist,3H pyrilamine, to the plasma membrane-enriched fraction was compared to the binding to crude membrane preparations. Both the plasma membrane-enriched fraction and the crude membrane preparation had similar Kd's for the histamine antagonist, but the plasma membrane-enriched fraction had a threefold greater binding capacity, reflecting the relative enrichment of plasma membranes of the preparation. Thus, a method has been developed for the isolation of plasma membranes from human peripheral lung which should provide material for a variety of biochemical and pharmacological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868524
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  • 2
    Electronic Resource
    Electronic Resource
    Calmodulin-like activity in a mineralising tissue: The rat molar tooth germ (1981)
    Springer
    Calcified tissue international 33 (1981), S. 545-548 
    Details
    ISSN: 1432-0827
    Keywords: calmodulin ; calcium ; mineralisation ; tooth germ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Calmodulin, a calcium binding protein, has been implicated in the regulation of many calcium-dependent biological processes. Since calcium has an important role in hard tissue genesis, both at intra- and extracellular levels, we anticipate that calcium binding proteins may modulate this process. The present study investigated a mineralising tissue, the rat molar tooth germ, to determine the presence of calmodulin-like activity. A heat-treated cell-free extract of tooth germs provided enhancement of Ca2+-dependent Mg2+-ATPase and 3′:5′-nucleotide phosphodiesterase activity. No enhancement occurred in the absence of calcium or in the presence of trifluoperazine. SDS-polyacrylamide gel electrophoresis of this extract revealed a protein band of approximately 18,000 mol. wt. These findings indicate the presence of calmodulin-like activity in rat molar tooth germs and support the proposal that calcium and calcium binding proteins, in particular calmodulin, have a major regulatory role in the biology of mineralising tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409487
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  • 3
    Electronic Resource
    Electronic Resource
    COMMENTARY ON “PRODUCTION/OPERATIONS MANAGEMENT: AGENDA FOR THE '80s” (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Decision sciences 12 (1981), S. 0 
    Details
    ISSN: 1540-5915
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1540-5915.1981.tb00109.x
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  • 4
    Electronic Resource
    Electronic Resource
    Rose mosaic disease: symptoms induced in roses by graft inoculation with both prunus necrotic ringspot and apple mosaic viruses (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 33 (1984), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Symptoms induced in rose by single isolates of the cherry serotype of prunus necrotic ringspot virus (PNRSV) and an apple serotype (apple mosaic virus; ApMV) were characteristically different, and appeared at different times throughout the growing season according to the ambient temperature. These features remained discrete, even in roses infected by both viruses and were shown by immunospecific electron microscopy to be a reliable indication of infection by either virus.However, cross-protection between the two isolates was not reciprocal; mixed infections were established only when roses were simultaneously graft-inoculated with ApMV and PNRSV, or when PNRSV-infected roses were supei-infected with ApMV. The significance of these results in relation to the possible natural occurrence of mixed infections in rose or of isolates of intermediate serotype is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1984.tb02635.x
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  • 5
    Electronic Resource
    Electronic Resource
    In Vivo and In Vitro Localization of Leishmania within Macrophage Phagolysosomes: Use of Colloidal Gold as a Lysosomal Label (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The interaction of Leishmania with lysosomes within macrophages in vivo has been investigated. Lysosomes labeled with colloidal gold in vivo fused with phagocytic vacuoles containing Leishmania amastigotes within the macrophages of infected footpad tissue of BALB/c mice. This localization of Leishmania within macrophage phagolysosomes in vivo is the first confirmation for any obligate intracellulaire protozoon that parasite-lysosome interactions in vitro occur in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02839.x
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  • 6
    Electronic Resource
    Electronic Resource
    Photoperiodism and rhythmic response to light (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 5 (1982), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Seedlings of Pharhitis nil show a circadian rhythm in the capacity to flower in response to the timing of a second red light pulse given at various times after a first saturating exposure to red when this is given together with a benzyladeninc spray. There are also changes in the photon irradiance required for half maximum response to the second red pulse.The photochemical properties of phytochrome in the photoperiodically sensitive cotyledons were also shown to change rhythmically. Oscillations in both pr→ Pfr and Pfr→ Pr photoconversion characteristics persisted over at least two circadian cycles with a periodicity of about 12 h. There were, however, no significant oscillations in either Pfr peak absorbance or in Δ(ΔA). The changes in sensitivity for the photoconversion of Pr→ Pfr did not parallel the much larger changes in sensitivity of the flowering response to red light. The amplitude of the Pr→ Pfr rhythm was at least as great as that for Pr→ Pfr, but the flowering response to far-red light was not rhythmic, nor was there any large change in sensitivity. The changes in photoconversion properties may reflect a basic biochemical oscillation which affects both photoreceptor properties and sensitivity to photoreceptor input.There was also a marked rhythm in the Pfr/P ratio that would be established by a saturating pulse of red light and this too may have affected the flowering response to such a pulse.Far-red light inhibited flowering when given at any time during the inductive night. After 14 h in darkness, Pfr could still be measured in the cotyledons and it was concluded that far-red light inhibited flowering by removing Pfr As red light also inhibited flowering at this time, there may be two pools of phytochrome with different kinetic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1982.tb00939.x
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  • 7
    Electronic Resource
    Electronic Resource
    Circulating Immune Complexes and Antisperm Antibodies in Yasectomized and Vasovasostomized Rhesus Macaques (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 438 (1984), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1984.tb38317.x
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  • 8
    Electronic Resource
    Electronic Resource
    An overview of sedimentation on the amazon continental shelf (1984)
    Springer
    Geo-marine letters 4 (1984), S. 207-210 
    Details
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Sedimentological, geochemical, and physical-oceanographic studies of the Brazilian continental shelf near the Amazon River help provide a broad understanding of this major sediment dispersal system. Amazon River sediment accumulates as a subaqueous delta, with the most rapid accumulation (∼10 cm/yr) occurring near the seaward edge of the topset beds and in the foreset beds. Amazon River sediment is dispersed northwestward along the shelf and is transported beyond the Brazilian border. Radiographic studies of sediment cores delineate three sedimentary environments: interbedded mud and sand, faintly laminated mud, and bioturbated mud. The distribution of these environments is a function of proximity to the river mouth and of sediment accumulation rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02281707
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  • 9
    Electronic Resource
    Electronic Resource
    Chronic exposure of goldfish-derived cell cultures to toxaphene alters the replication of Goldfish Virus-2 (1982)
    Springer
    Bulletin of environmental contamination and toxicology 29 (1982), S. 731-733 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606114
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  • 10
    Electronic Resource
    Electronic Resource
    Toxicity and intracellular localization of carbaryl and 1-naphthol in cell cultures derived from goldfish (1983)
    Springer
    Bulletin of environmental contamination and toxicology 30 (1983), S. 99-104 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01610105
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