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  • 1
    Electronic Resource
    Electronic Resource
    Lytic enzymes in autolysis of Botrytis cinerea (1983)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 19 (1983), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1983.tb00532.x
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  • 2
    Electronic Resource
    Electronic Resource
    Protoplast production from filamentous fungi with their own autolytic enzymes (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 24 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Production of protoplasts in different genera of filamentous fungi with their own lytic enzymes obtained from autolyzed cultures, as well as the regeneration of these protoplasts, has been studied. The results support the idea that the use of these autolytic enzymes could be a general method of production of protoplasts from filamentous fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01320.x
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of catalase and peroxidase in Saccharomyces cerevisiae: a plausible site of action of an intracellular regulatory factor (1983)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 18 (1983), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1983.tb00442.x
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  • 4
    Electronic Resource
    Electronic Resource
    CORN (Zea mays) KERNEL HARDNESS AS AN INDEX OF THE ALKALINE COOKING TIME FOR TORTILLA PREPARATION (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A functional relationship has been established between the hardness (peak bioyield point) of raw corn kernels and the time needed for the cooking process. The alkaline cooking index (sensation of softness of kernels, cooked appearance of kernel endosperm, and huh disintegration) was found to have an equivalent kernel hardness. The hardness of raw kernels and the cooking time for four different corn varieties were significantly different (P 〈 0.0001). Within a corn variety, higher calcium hydroxide concentration slightly decreased the cooking time. The linear relationship between variety initial hardness and time at the terminal point of processing makes possible the prediction of the cooking time of a corn variety when its initial hardness is known.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb03793.x
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  • 5
    Electronic Resource
    Electronic Resource
    ASTRINGENCY IN AN INTERMEDIATE MOISTURE BANANA PRODUCT (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the development of an intermediate moisture banana product, it was found that ripe banana halves dehydrated to a moisture content of about 30%, following blanching and/or sulfiting, are remarkably astringent. In order to understand the cause of this phenomenon, total phenolics, leucoanthocyanins and vanillin-reacting compounds were determined and found to be more extractable in astringent semidried bananas. Light microscopy observations showed that both blanching and sulfiting contribute to the leakage of tannin-like compounds from the latex cells. Overall results conform with a mechanism by which appearance of astringency in semidried banana is due to diffusion of the astringency-causing agents from the latex cells to the surrounding tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1977.tb14459.x
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  • 6
    Electronic Resource
    Electronic Resource
    THE FATE OF AFLATOXINS IN THE PREPARATION OF PROTEIN CONCENTRATES AND ISOLATES FROM CONTAMINATED PEANUT AND COTTONSEED FLOURS (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the assistance of the laboratories of six major soybean protein producers, simulated commercial protein concentrate and isolate processes were applied to aflatoxin-contaminated peanut and cottonseed flours to determine the fate of the aflatoxins. Processes for producing, concentrate by washing the flour at the protein isoelectric point (pH 4.5) were ineffective in separating the aflatoxin from the products, but a process using an aqueous alcohol wash accomplished a 90% reduction in the aflatoxin level of the concentrate compared to the flour. Processes for producing isolate by protein dissolution in alkali and its recovery by precipitation at the isoelectric point resulted in some aflatoxin loss but an increase in the aflatoxin concentration associated with the protein isolate compared to the original flour. However, a process applying carbon adsorbent to the alkaline solution accomplished a 90% reduction in the aflatoxin level of the isolate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1976.tb14435.x
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  • 7
    Electronic Resource
    Electronic Resource
    Ground-Water Age Distribution in Madrid Basin, Spain (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 20 (1982), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The alluvial deposits that occupy the Madrid Basin in central Spain form an aquifer system covering an area of 5,000 km2 (2,000 mi2) and with thicknesses of 2,000 m (6,600 ft) or more. Average annual precipitation is 500 mm (15 in.) and average annual temperature is 15°C (59°F). The precipitation is sufficient to provide a net surplus to ground-water recharge which, in turn, supports dry weather flow of major streams in the basin. A distribution of surface recharge and hydraulic conductivities were obtained from a previous study utilizing a two-dimensional finite-difference model of the same vertical cross section as this study. In this study a flow net and a discrete-state compartment (or “mixing-cell”) model were employed to calculate the age distribution of ground water circulating through the aquifer. Carbon-14 decay ages were determined for nine ground-water samples taken from eight locations. The ages obtained with the flow net and with the mixing-cell models are mutually consistent and generally agree with the carbon-14 decay ages. The calculated ages range from zero at the recharge boundaries to over 100,000 years at discharge boundaries in stream channels. The results obtained are to be regarded as preliminary. Their principal value will be to guide future C-14 field sampling programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1982.tb01388.x
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  • 8
    Electronic Resource
    Electronic Resource
    A RADIOASSAY FOR MACROPHAGES AND ITS APPLICATION FOR TESTING MACROPHAGE MEMBRANE ANTIGENS (1975)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 2 (1975), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Monolayers of mouse peritoneal macrophages actively incorporated 14C-labelled uridine, an RNA precursor. This uptake did not seem to be due to in vitro activation or stimulation of the cells but rather to their high RNA metabolic activity. 14C-uridine uptake by the unstimulated adherent cells was a characteristic property of macrophages, because it was not reduced when the cells were (i) pretreated with trypsin (to detach remaining lymphocytes), (ii) precultured for several days (to allow PMN to die off) and (iii) pretreated with anti-mouse immunoglobulin or anti-θ serum plus complement.Pretreatment of macrophage monolayers with antibody plus complement, followed by a measurement of their 14C-uridine uptake could be used to test for anti-macrophage activity, for instance in certain anti-B cell or anti-T cell sera, or to detect cell surface antigens on macrophages. With this technique we demonstrated the presence of H-2 and Ia alloantigens on peritoneal macrophages. The presence of Ia antigens on macrophages may be of particular biological significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1975.tb00548.x
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  • 9
    Electronic Resource
    Electronic Resource
    Surface Charge of Trypanosoma cruzi. Binding of Cationized Ferritin and Measurement of Cellular Electrophoretic Mobility (1977)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 24 (1977), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS The surface charge of epimastigote and trypomastigote forms of Trypanosoma cruzi was evaluated by means of binding of cationized ferritin to the cell surface as visualized by electron microscopy, and by direct measurements of the cellular microelectrophoretic mobility (EPM). Epimastigote forms had a mean EPM of -0.52 μm.s-1.V-1.cm and were lightly labeled with cationized ferritin. In contrast, bloodstream trypomastigotes had a much higher EPM (-1.14), and the surface was heavily labeled with cationized ferritin. When trypomastigotes from stationary phase cultures were isolated on DEAE cellulose columns, the mean EPM was found to be significantly lower (-0.63), and labeling with cationized ferritin decreased. With a mixed population containing epimastigote, trypomastigote, and intermediate forms, EPM values ranging between -0.70 to -1.14 were found. From these observations we conclude that there is a definite increase in negative surface charge during development from epi- to trypomastigote forms of T. cruzi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1977.tb04762.x
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  • 10
    Electronic Resource
    Electronic Resource
    Entamoeba histolytica: Collagenolytic Activity and Virulence (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Several axenic strains of pathogenic and nonpathogenic Entamoeba histolytica were tested for their capacity to digest native radioactive type I collagen gels and to produce liver abscesses when injected into the liver of newborn hamsters. The results demonstrate that the pathogenic strains of amebas (HM1:IMSS, HM3:IMSS, HM38:IMSS, and HK9) have a collagenolytic activity that closely correlates with their in vivo capacity to produce liver lesions. The nonpathogenic isolate (Laredo) did not show collagenolytic activity and failed to produce lesions in the liver of newborn hamsters. The results also demonstrate that type I collagen obtained from rodents and cats is degraded less by amebic collagenase than is bovine collagen, which is similar to human collagen. These findings suggest that species susceptibility to invasive infection may depend, among other factors, on the characteristics of the extracellular components of host tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb02995.x
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