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  • Blackwell Publishing Ltd  (15)
  • American Institute of Physics (AIP)
  • American Meteorological Society (AMS)
  • 1
    Electronic Resource
    Electronic Resource
    Transformation of Lactobacillus delbrückii ssp. lactis by electroporation and cloning of origins of replication by use of a positive selection vector (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 78 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this communication we report the first successful transformation of Lactobacillus delbrückii ssp. lactis WS97 with plasmid DNA by means of electroporation and describe the optimization of the transformation procedure for this strain. Efficiencies of electroporation varied between 102 to 104 transformants per μg pGK12, depending on the strain from which the DNA was isolated. The application of electroporation in molecular cloning was achieved by using the newly constructed origin screening vector, pAZ8. The replication origins of two cryptic plasmids were cloned. These plasmids were isolated from a thermophilic Lactobacillus strain Lb. delbrückii ssp. lactis WS97 and a mesophilic Lactobacillus strain Lb. casei NCDO151 which are both used in the dairy industry as starter cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04444.x
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  • 2
    Electronic Resource
    Electronic Resource
    DEVELOPMENT OF Yersinia enterocolitica ON RAW AND COOKED BEEF AND PORK AT DIFFERENT TEMPERATURES (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Yersinia enterocolitica counts of inoculated beef and pork as determined from confirmed isolates picked from trypticase soy agar plates were similar to the counts of confirmed enamel-black colonies on bismuth sulfite agar plates. With three strains of Y. enterocolitica increases in count occurred on raw beef held over a 10-day period at 0–1°C. When inoculated raw or cooked beef and pork were stored at 7°C (0–10 days) or at 25°C (0–24 hr) large increases in Y. enterocolitica count occurred. At 25°C the increases in Y. enterocolitica counts were somewhat greater on cooked than on raw products. These differences in count may have been caused by (a) differences in the physicochemical characteristics of the meat (raw vs cooked) and/or (b) differences in the level and type of microbial flora that developed on these products. In addition to Y. enterocolitica, Staphylococcus and Micrococcus spp. often were dominant on cooked products, Pseudomonas and Microbacterium spp. on raw meats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1977.tb14455.x
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  • 3
    Electronic Resource
    Electronic Resource
    Yersinia enterocolitica-LIKE ORGANISMS FROM VACUUM-PACKAGED BEEF AND LAMB (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Characteristics are presented for Yersiniu enterocoliticu-like organisms isolated from vacuum-packaged beef and lamb stored for 21-35 days at l-3°C. Isolation of this organism was more frequent after 28 days of storage under vacuum conditions than under nonvacuum conditions (leaker packages). A higher incidence of isolates was obtained from cuts packaged under high vacuum conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1976.tb14436.x
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation, Purification, and Characterization of Lipase Isoenzymes from a Technical Aspergillus niger Enzyme (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A qualitative screening revealed the occurrence of lipase, esterase, protease, amylase, endo-1,4-β-D-glucanase, xylanase, pectinmethylesterase, polygalacturonase, catalase, β-D-glucosidase and β-D-galactosidase activities in the technical Aspergillus niger enzyme under study (Lipase 2212 D, Röhm). The isolation and purification of lipolytic activities were performed by combination of DEAE-Trisacryl M ion exchange chromatography, Sephadex G 50 gel filtration and hydrophobic chromatography using Phenylsepharose CL-4B. The individual purification steps were checked by specific enzyme visualization in ultrathin agar gels after ultrathin-layer isoelectric focusing (UIEF). Two UIEF homogeneous lipase isoenzymes (I and II) were isolated and characterized by the following parameters: isoelectric points (I: 4.0; II. 3.5); molecular weights (I: 31000 daltons; II: 19000 daltons); carbohydrate contents (I: 6%; II: 9%) and compositions; pH optima (I, II: 5-6); substrate specificities and various effectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb10574.x
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of Methods for Quantitation of Free Fatty Acids in Cheese (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A comparison of three relatively rapid methods of determining free fatty acids concentrations in cheese was performed by correlating the results with a more laborious but accurate gas chromatographic (GC) technique. Acid Degree Values were found to correlate poorly with the GC data, particularly for short chain fatty acids. Values obtained using a Copper Soap Method correlated closely with the GC data for total and long chain fatty acids, but did not correlate as well with short chain fatty acids levels. The Extraction-Titration Method was found to be a simple and reliable technique that yielded values which correlate closely with short chain fatty acid concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb07878.x
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  • 6
    Electronic Resource
    Electronic Resource
    Lamb dip and infrared–radio frequency double resonance spectroscopy of 188OsO4 (1991)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 94 (1991), S. 2509-2512 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The frequency of seven rovibrational transitions in the ν3 band, Q branch of 188OsO4 have been measured with high accuracy. The spectrometer used consists of two CO2 lasers [∼3 W on the 10R(0)], the first of which is stabilized on the 4.3 μm fluorescence Lamb dip of the CO2 and the second on the saturated absorption dip of the rovibrational transitions of 188OsO4. The OsO4 transition frequencies are obtained combining the known frequency of the first laser with the measured frequency of the beat note between the two laser beams. For the assignment of the transitions the recent Fourier transform analysis by Bobin et al. [J. Mol. Spectrosc. 122, 229 (1987)] was used. The access to the 10R(0) laser line has made it possible to investigate, for the first time, the Q branch of the ν3 band of OsO4 in a sub-Doppler regime. Furthermore, the tetrahedral fine structure in the ground and in the ν3 state has been analyzed using the infrared–radio frequency double resonance technique. The tetrahedral splitting constants, Dt for the ground state and Z3t for the ν3 state, have been calculated with higher accuracy than in previous works.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.459875
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  • 7
    Electronic Resource
    Electronic Resource
    Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 217 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A fast and reliable Multiplex-PCR assay was established to identify the species Lactobacillus johnsonii. Two opposing rRNA gene-targeted primers have been designed for this specific PCR detection. Specificity was verified with DNA samples isolated from different lactic acid bacteria. Out of 47 Lactobacillus strains isolated from different environments, 16 were identified as L. johnsonii by PCR. The same set of strains was investigated with five alternative molecular typing methods: enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), repetitive extragenic palindromic PCR (REP-PCR), amplified fragment length polymorphism, single triplicate arbitrarily primed PCR, and pulsed-field gel electrophoresis in order to compare the discriminatory power of these methods. The reported data strongly support the highly significant heterogeneity among all L. johnsonii isolates, potentially linked to their origin of isolation. The use of species-specific primers as well as rapid and highly powerful PCR-based molecular typing tools (namely ERIC- and REP-PCR techniques) should be respectively envisaged for identifying, differentiating and monitoring L. johnsonii strains from various environmental samples, for product monitoring, for species tracing in clinical studies as well as bacterial profiling of various microecological or gastrointestinal environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11468.x
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular analysis of ancient microbial infections (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 213 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host–pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic analyses of pathogens. In the last few years, a variety of bacterial, protozoal and viral infections have been detected in ancient tissue samples by amplification and characterization of specific DNA fragments. This holds particularly true for the identification of the Mycobacterium tuberculosis complex, which seems to be more robust than other microbes due to its waxy, hydrophobic and lipid-rich cell wall. These observations provided useful information about the occurrence, but also the frequency of tuberculosis in former populations. Moreover, these studies suggest new evolutionary models and indicate the route of transmission between human and animals. Until now, other pathogens, such as Mycobacterium leprae, Yersinia pestis, Plasmodium falciparum and others, have occasionally been identified – mostly in single case studies or small sample sizes – as well, although much less information is available on these pathogens in ancient settings. The main reason therefore seems to be the degradation and modification of ancient DNA by progressive oxidative damage. Furthermore, the constant risk of contamination by recent DNA forces to take time and cost effective measures and renders the analysis of ancient microbes difficult. Nevertheless, the study of microbial ancient DNA significantly contributes to the understanding of transmission and spread of infectious diseases, and potentially to the evolution and phylogenetic pathways of pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11298.x
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  • 9
    Electronic Resource
    Electronic Resource
    Measurement of high-frequency rotational transitions of H2O+ in its ground state by far-infrared laser magnetic resonance (LMR) spectroscopy (1998)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 109 (1998), S. 9744-9752 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Thirteen new rotational transitions of H2O+ in the (0,0,0) level of the X˜ 2B1 state have been measured in the wavenumber region between 80 and 200 cm−1 (50 and 120 μm) by far-infrared laser magnetic resonance (LMR) spectroscopy. LMR data measured previously between 25 and 90 cm−1 (110 and 400 μm), as well as optical and infrared combination differences, have been combined with the new LMR data in a weighted least-squares analysis using an A-reduced expression of the rotational-fine structure Hamiltonian. Thirty-two molecular constants were simultaneously determined, some sextic centrifugal distortion parameters and some quartic and sextic spin-rotation parameters for the first time. From this improved set of molecular parameters, very accurate calculations of rotational term values and zero-field predictions of the 111–000 transition, including hyperfine structure, have been performed. Moreover, the electronic g-tensors and the hyperfine coupling constants are consistent with ab initio calculations which had been carried out for these constants. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.477644
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  • 10
    Electronic Resource
    Electronic Resource
    Aggregate model of liquids (1997)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 107 (1997), S. 8697-8705 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Atomic-force pictures reveal a heterogeneous microstructure at the surface of glassy layers which should be similar to one of the many equivalent microstructures a liquid is running through. These microstructures are described with the aid of a kinetic model of reversible aggregation which goes back to formulations as used in the description of living polymerization or aggregation of polymers in solution. Aggregates are considered as dynamic subsystems wherein collective modes of motions are excited. Fluctuations of the aggregates, densely packed in a disordered pattern, leads to a broad size distribution which happens to be controlled by Boltzmann's factor. The disordered structure within the aggregates themselves is optimized, reduced aggregate energy and entropy should be equal. Symmetries are deduced which elucidate many universal properties of the dynamic microstructure of liquids. Thermodynamic properties like the specific heats of aggregation in liquids or the dependence of the glass transition of homologues of linear atactic polystyrene are consistently described. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.475022
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