ALBERT

Notes: The paper presents an ecological risk-assessment methodology which (a) addresses surface-water exposures to aquatic mammals and birds, and (b) accounts for long-term accumulation and biomagnification of pollutants. The methodology has been adapted from those developed in the USA for compliance with US Environmental Protection Agency requirements, and combines predicted or measured surface-water concentration data with information on the natural history of wildlife species and chemical-specific bioaccumulation factors, to determine whether estimated exposures would exceed toxicological criteria. Whilst the methodology is applied to metals in leachate discharges from landfill sites, the general approach is applicable to other industrial sites. The approach could provide the basis for a shift towards more numerical risk-based ecological assessments in a wide variety of contexts.

Notes: In a family study of coeliac disease, HLA types in fifty-three patients and their relatives were examined. There are no differences in HLA frequencies between child and adult patients. Comparison with a random series of normal controls shows increases frequencies in patients of HLA-A1 and B8, while the family material shows that there is also an excess of haplotype I-8. The excess of homozygotes is thought not to be a factor in the aetiology. Intrafamilial analysis shows that only B8 is significantly associated with the disorder. It is argued that the HLA association does not indicate a ‘coeliac gene’ but that the B8 allele is a major gene in a polygenic system affecting the disorder.

Notes: Abstract Textural relationships between porphyroblasts of biotite and garnet in metasediments in the Nordkinn Peninsula area of the Finnmarkian Caledonides of North Norway are apparently complex. There is evidence for two textural zones in both mineral phases and superficially the development of these appears to have overlapped, at least in part, in time and space. This apparently complex porphyroblast growth history can be considerably simplified if only one period of garnet growth occurred and if different inclusion fabrics developed where garnet replaced biotite porphyroblasts and where it overgrew the matrix foliation. The possibility that porphyroblasts with textural evidence for multiphase growth histories actually grew during a single crystallization event is of importance in the interpretation and elucidation of tectonometamorphic relationships.

Notes: Two experiments examined the effects of different defoliation treatments in spring on sward morphology and animal performance in mid-season and late season. Three treatments were applied in both experiments: Control (C), sward grazed by cows in spring to 6–8 cm grass height. Grazed Aftermath (GA). sward grazed by cows in spring to 3–4cm and allowed to regrow before being grazed by summer-calving cows, Silage Aftermath (SA), sward not grazed in spring, but a primary cut taken and the sward allowed to regrow before being grazed by summer-calving cows. The aim of treatment GA was to produce a sward with a high tiller density and high intake characteristics to meet the forage intake requirements of continuously grazed summer-calving cows, without resorting to offering forage buffers. Experiment 1 was conducted in 1989 on a sandy loam soil and Experiment 2 in 1990 on a heavy loam soil.In both experiments the GA treatment led to high live tiller density and live: dead tiller ratios compared with the C and SA treatments. Differences in sward morphology were also detected by applying double normal distribution analyses to measurements of grass height. The GA treatment also increased sward herbage mass and, to a limited extent, herbage metabolizable energy and crude protein contents. The results from Experiment 1 suggested that these sward effects lead to increased herbage dry-matter intake (as estimated by the n-alkane technique) and milk yield in cows grazing the GA sward. However, in Experiment 2, where conditions for grass growth in mid-season were more favourable than in Experiment 1, the differences in sward morphology produced in spring were quickly lost in June and July. There were therefore no differences in herbage intake or milk yield in the second experiment. Herbage intakes (kgDMd−1± s.e.d) estimated in July for cows on treatments C, GA and SA were 11·0, 13·4, 10·1 ± 2·16 for Experiment 1 and 10·7, 11·1, 11·2 ± 2·32 for Experiment 2. Average milk yield (kgd−1± s.e.d.) for cows on treatments C, GA and SA were 26·1, 28·0, 25·6 ± 0·31 (Experiment 1) and 28·5, 27·3, 28·4 + 0·58 (Experiment 2).The results suggested that acceptable milk yields can be obtained from grazing summer-calving cows, without offering forage buffers, by applying high stocking rates (low grass heights) in spring. However, the benefits of this manipulation could be lost by lax grazing in mid-season.

Notes: Over a 24-week period, three groups of dairy cows were continuously stocked at 8, 10 or 12 cows ha-1 between morning and afternoon milkings, and overnight were housed and offered grass silage ad libitum. Due to a prolonged drought, sward heights only averaged 4·1 cm.The increase in daytime stocking rate led to a decline in herbage intake, and increases in silage intake. At the highest stocking rate (12 cows ha-1), the silage intake failed to compensate for the reduced herbage intake. Consequently the total dry matter and estimated metabolizable energy intakes were lower than for the 8 and 10 cows ha-1 treatments. Milk yields and milk composition were not significantly affected by treatment but the 12 cows ha-1 stocking rate gave the lowest milk and milk solids yields.The utilized metabolizable energy (UME) on the grazed swards was greatest for the 10 cows ha-1 treatment. The sward cut to provide the silage had a UME level (GJ ha-1) 32% greater on average than the grazed swards during the same growth period. The total areas utilized for grazing and silage production for 8, 10 and 12 cows ha-1 were 0·240, 0·224 and 0·215 ha respectively. Fat and protein yields per unit area were greatest for the 10 cows ha-1 group.

Notes: Twelve cows were used in a cyclic changeover design experiment to examine the effects of feeding fodder beet (variety Kyros) at three levels (0, 2 and 4 kg dry matter (DM) d−1) with two levels of concentrate feeding (4 and 8 kg DM−1). Silage was offered ad libitum. At the start of the experiment the mean number of days after calving was 46 and the mean live weight 516 kg. The experiment consisted of four 3-week periods with intake and performance measurements during the last week of each period.An in vitro analysis of the feeds for the silage, fodder beet and concentrate respectively was: DM (g kg-−1) 189, 163, 860; crude protein (g kg DM−1) 143,76,201; metabolizable energy (MJ kg DM−1) 104, 133, 13–5.Silage DM intake decreased but total DM intake increased when fodder beet was fed. The DM intakes (kg d−1) for treatments (fodder beet/ concentrate) 0/4, 2/4, 4/4, 0/8, 2/8, 4/8 respectively were: silage DM 91, 79, 78, 83, 70 and 68 (s.e.d. =0.31); and total DM 130, 138, 156, 162, 166, 183 (s.e.d. = 0.36). Feeding fodder beet had no significant effects on milk yield or milk composition, but there was a significant increase in milk protein yield when fodder beet was included in the ration. There were no significant interactions between level of fodder beet feeding and concentrate level. Increasing the level of concentrate feeding led to a highly significant increase in milk yield, milk protein content and yield of milk constituents. The yields for treatments 0/4, 2/4,4/4,0/8, 2/8,4/8 respectively were: milk yield (kg d−1) 206, 204,21 8, 241,235, 244 (s.e.d. = 0–58); fat yield (g d−1) 827, 793, 885, 954, 936, 954 (s.e.d. = 394); and protein yield (g d−1) 622, 628, 679, 774, 777, 814 (s.e.d. = 179). There were no significant differences in milk yield and milk composition when fodder beet was included in the diet which may have been due to the low crude protein content of the diet.

Notes: In two changeover design experiments, fifteen early- and sixteen late-lactation cows were used to investigate the effects of offering food beet with ad libitum grass silage and concentrates with different CP content on milk yield and quality. In Experiment 1 (early lactation) cows were offered no fodder beet (0) or 4 kg DM d−1 (4) in conjunction with one of three concentrates containing 159, 191 or 244g CP kg−1 DM (L.M.H.). Treatments were therefore 1L/0, 1L/4, 1M/0, 1M/4, 1H/0 and 1H/4. In Experiment 2 (late lactation) cows were offered the same level of fodder beet in conjunction with two concentrates containing 129 and 229 (L,H) g CP kg−1 DM. Treatments were therefore 2L/0, 2L/4, 2H/0 and 2H/4.In both experiments feeding fodder beet reduced silage DM intakes (P 〈 0·001) and increased total DM intake (P 〈 0·05 to P 〈 0·001). The substitution rate (r) ranged from 0·46 to 0·59kg of silage DM (kg−1 fodder beet DM).In Experiment 1, fodder beet tended to increase milk yield, composition and yield of constituents, but the effect was statistically significant for milk protein content only (P 〈 0·01). In Experiment 2, milk yields for 2L/0, 2L/4, 2H/0 and 2H/4 were 11·3, 12·1, 11·7 and 12·5 kg d−1 respectively (s.e.d. 0·43, non-significant), fat contents were 44·4, 47·3, 44·3 and 46·8g fat kg−1 respectively (s.e.d. 0·73, P 〈 0·001), protein contents were 34·3, 35·6, 35·3 and 36·2 g protein kg−1 respectively (s.e.d. 0·28, P 〈 0·001), fat yields were 494, 574, 512 and 579 g fat d−1 respectively (s.e.d. 20, P 〈 0·001) and protein yields were 385, 426, 407 and 442 g protein d−1 (s.e.d. 13, P 〈 0·01) respectively.Increasing CP in the concentrate significantly increased milk yield in Experiment 1 (23·9, 22·5, 23·5, 23·8, 26·2, 26·5kg d−1 for 1L/0, 1L/4, 1M/0, 1M/4, 1H/0 and 1H/4 respectively, P 〈 0·05). Higher CP in concentrate also resulted in significantly increased milk protein yield in early-lactation (P 〈 0·001) and milk protein content in late-lactation (P 〈 0·01) cows. There was a significant interaction between fodder beet and concentrate CP content for milk protein yield (P 〈 0·001) in Experiment 1.

Notes: Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10−6 MJ kg MD−1 and 160 and 191 g kg DM−1 respectively. The straw mix had an ME content of 10−1 MJ kg DM−1 and CP content of 134 g kg DM−1. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d−1) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd−1) 163, 155, 163.Animal performance was, for treatments G, Si and St respectively: milk yield (kg d−1) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg−1) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg−1) 35·3, 32·9, 33·4 (s.e.d. 0·76).