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  • 1
    Electronic Resource
    Electronic Resource
    Phage release from biofilm and planktonic Staphylococcus aureus cells (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 252 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The ability of pathogenic staphylococci to form biofilms facilitates colonization and the development of chronic infections. Therapy is hampered by the high tolerance of biofilms towards antibiotic treatment and the immune system. We found evidence that lysogenic Staphylococcus aureus cells in a biofilm and in planktonic cultures spontaneously release phages into their surroundings. Phages were detected over a much longer period in biofilm cultures than in planktonic supernatants because the latter were degraded by secreted proteases. Phage release in planktonic and biofilm cultures was artificially increased by adding mitomycin C. Two morphologically distinct phages in the S. aureus strain used in this work were observed by electron microscopy. We postulate that phage-release is a frequent event in biofilms. The resulting lysis of cells in a biofilm might promote the persistence and survival of the remaining cells, as they gain a nutrient reservoir from their dead and lysed neighboring cells. This might therefore be an early differentiation and apoptotic mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.08.048
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  • 2
    Electronic Resource
    Electronic Resource
    Nutritional Properties and Metabolic Studies of Acylated Beef Heart Myofibrillar Proteins (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Beef heart myofibrils were acylated with several concentrations of acetic (AA) and succinic (SA) anhydride, and digestibility and utilization of the myofibrillar proteins were determined. Results for in vitro hydrolysis of the untreated and acylated proteins varied, depending on the enzyme(s) used in the analysis. Rat protein efficiency ratios and net protein ratios for untreated (PER = 2.83, NPR = 116) and acetylked (PER = 2.55, NPR = 110) proteins were greater than for casein (PER = 2.50, NPR = 100), whereas values for succinylated proteins (PER = 2.36, NPR = 87) were less than for casein. Most of the radioactivity recovered after 24 hr from rats fed 14C-acylated myofibrillar proteins was in expired CO2; 62.8% for 14C-acetylated and 45.8% for 14C-succinylated proteins. Rats acclimated to an acylated protein diet for 28 days showed improved metabolism of 14C-acetylated protein and decreased metabolism of 14C-succinylated proteins; 75.7% and 38.1% recovery as expired CO2, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1982.tb11023.x
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  • 3
    Electronic Resource
    Electronic Resource
    Chemical Modification and Functional Properties of Acylated Beef Heart Myofibrillar Proteins (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Beef heart myofibrils were acylated with 0.1, 0.3, 0.6, 0.8, 1.0, 1.5, 2.0, 3.0, and 5.0 mmoles anhydride/g protein, at pH 8.0–8.5 and 2–3°C, with acetic anhydride (AA), succinic anhydride (SA), cis,cis,cis,cis-tetrahydrofuran-2,3,4,5-tetracarboxylic dianhydride (FA), and 1,2,4-benzenetricarboxylic anhydride (BA). The anhydride reacted with e-amino groups of lysine, sulfhydryl groups, and hydroxyl groups of tyrosine, serine, and threonine. Chemically modified beef heart myofibriliar proteins were superior to native heart myofibriliar proteins in solubility, emulsifying capacity, emulsion activity, and emulsion stability in a low salt solution of 0.1M NaCl, 0.05M potassium phosphate at pH 7.4 and 6.0. Protein modified with 0.5 mmole anhydride/g protein in 0.2M NaCl had a solubility greater than unmodified proteins in 0.6M NaCl at pH 7.4. Chemical modification also altered the pH-solubility profile. The chemically modified beef heart myofibrillar proteins exhibited an emulsifying capacity at pH 6.0 and 7.4 that was greater than that of the native proteins at pH 7.4. The recommended extent of acylation for modifying beef heart myofibrils on a gram protein basis is 0.6 mmole AA, 1.5 moles SA, 0.6 mmole FA, and 0.6 mmole BA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1981.tb02999.x
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  • 4
    Electronic Resource
    Electronic Resource
    A Rapid Method for Separation and Quantitation of D-Malic Acid in Fruit Juice (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 55 (1990), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A HPLC method using an aqueous mobile phase containing the chiral ligand-exchanger Cu II-L-valine complex at pH 5.5 with a polystyrene divinyl-benzene copolymer column was used to resolve D-malic acid in apple, pear, and Concord grape juices. D-malic acid was detected and quantitated at 330 nm in less than 15 min per sample. The detection limit appeared to be 2 mg/100 mL D-malic acid in 12 Brix juice, or 0.33% total malic acid in a typical apple juice containing 0.6 g/100 mL using the described procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1990.tb03583.x
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  • 5
    Electronic Resource
    Electronic Resource
    Assessing Pesticide Contamination to Ground Water: A Rapid Approach (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 29 (1991), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A methodology has been developed for identifying hazardous pesticides/site combinations threatening ground-water contamination. Screening methodologies are required to determine which locations and pesticides now in use should receive the greatest attention to safeguard the public health. The presented method uses a hazard to ground-water hydrogeological screening model (DRASTIC) and employs a one-dimensional pesticide transport model (CMLS). The method is an efficient and practical technique to identify where particular combinations of pesticides, water management practices, soils, and geology result in the greatest potential hazard to ground-water contamination. Use of the presented approach can reduce sampling needs and expense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1991.tb00573.x
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  • 6
    Electronic Resource
    Electronic Resource
    Improvement of Highway Safety I: Identification of Causal Factors Through Fault-Tree Modeling (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Risk analysis 15 (1995), S. 0 
    Details
    ISSN: 1539-6924
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: As the first article of a two-part series, the purpose of this paper is to examine the functional factors that contribute to automobile accident occurrence and to model the causation structure in the form of a fault-tree. The fault-tree model provides an intuitive framework for qualitatively decomposing possible pathways to accident occurrence. Fault-tree analysis also provides a statistical representation of how interacting driver, vehicle, and environmental factors contribute to the likelihood of automobile accident occurrence. The application of this model facilitates pinpointing those factors that most contribute to accident causation and subsequently enables the identification and comparison of potential crash avoidance technologies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1539-6924.1995.tb00323.x
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  • 7
    Electronic Resource
    Electronic Resource
    DAMINOZIDE RESIDUE PRESENT IN APPLE FRUIT AFTER LONG AND SHORT TERM EXPOSURE (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 11 (1991), S. 0 
    Details
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Daminozide residues were determined in ppm or ppb on ‘Starking', ‘Red King', ‘Rome’and ‘Golden Delicious’apples after repeated long term exposure (21 years) and on ‘Top Red’apples after short term (1 year) exposure. Application rates varied from 2.2 to 9.0 kg/ha for short term exposure and 2.2 to 4.5 kg/ha for long term exposure. Two and three years after cessation of the daminozide spray program no daminozide residues were found in ‘Top Red’regardless of the rate applied or analytical procedure used. Daminozide residues (〈1 ppm) were present in ‘Starking', ‘Red King', ‘Rome’and ‘Golden Delicious’one year after cessation of the spray program. The cultivar ‘Rome’had consistently higher daminozide residue levels followed by ‘Golden Delicious', ‘Red King’and ‘Starking'.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4565.1991.tb00056.x
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  • 8
    Electronic Resource
    Electronic Resource
    Uptake and Elimination of Isoflurane in Rabbit Tissue Studied with 19F NMR Spectroscopy (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 508 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb32955.x
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  • 9
    Electronic Resource
    Electronic Resource
    Best Estimated Aroma and Taste Detection Threshold for Guaiacol in Water and Apple Juice (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 70 (2005), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Alicyclobacillus acidoterrestris can produce sufficient guaiacol (methoxyphenol), a metabolic by-product of the bacterium, in apple juice to cause a detectable taint characterized by an antiseptic off-odor or distinct medicinal flavor and lingering aftertaste. Bacterial spoilage may not be visibly detectable. The objective of this study was to determine the best estimate threshold (BET) for detection of guaiacol in water and commercial pasteurized apple juice from concentrate using the forced-choice ascending concentration method of limits with an experienced 17-member sensory panel. The mean BET for aroma detection of guaiacol in water and apple juice was 0.48 ppb and 0.91 ppb, respectively. The mean BET for taste detection of guaiacol in water and apple juice was 0.17 ppb and 0.24 ppb, respectively. Individual aroma BET values ranged from 0.06 ppb to 4.71 ppb guaiacol in water and 0.17 ppb to 4.71 ppb for guaiacol in apple juice. Individual taste BET values ranged from 0.01 ppb to 4.71 ppb for guaiacol in water and apple juice. The taste BET was equal to or lower than the aroma BET for guaiacol in both water and apple juice for all panelists. There was about a 500-fold range in guaiacol taste detection between panelists, with some individuals exhibiting a BET value as low as 10 ppt (trillion). The information should be useful for developing quality assurance sensory methodology to evaluate potential apple juice flavor spoilage by Alicyclobacillus spp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2005.tb07201.x
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  • 10
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    Particle nucleation in the tropical boundary layer and its coupling to marine sulfur sources (1998)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1998-10-02
    Description: New particle formation in a tropical marine boundary layer setting was characterized during NASA's Pacific Exploratory Mission-Tropics A program. It represents the clearest demonstration to date of aerosol nucleation and growth being linked to the natural marine sulfur cycle. This conclusion was based on real-time observations of dimethylsulfide, sulfur dioxide, sulfuric acid (gas), hydroxide, ozone, temperature, relative humidity, aerosol size and number distribution, and total aerosol surface area. Classic binary nucleation theory predicts no nucleation under the observed marine boundary layer conditions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Clarke -- Davis -- Kapustin -- Eisele -- Chen -- Paluch I -- Lenschow -- Bandy -- Thornton -- Moore -- Mauldin -- Tanner -- Litchy -- Carroll -- Collins -- Albercook -- New York, N.Y. -- Science. 1998 Oct 2;282(5386):89-92.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉A. D. Clarke, V. N. Kapustin, K. Moore, M. Litchy, School of Ocean and Earth Science and Technology, University of Hawaii, Honolulu, HI, USA. D. Davis, F. Eisele, G. Chen, School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atl.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9756483" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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