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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 16 (1969), S. 0 
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: SYNOPSIS. Some aspects of the physiology of encystment of the soil amoeba Hartmannella castellanii in a replacement encystment medium consisting of 5 × 10-2 M MgCl2 have been investigated. It is suggested that measurement of the cellulose produced during encystment in the synthesis of the cyst wall is a more reliable measure of the process than other methods tried.The degree of encystment was dependent on the physiologic state of the amoebae and the composition of the growth medium, but the initial pH of the encystment medium (C. 4.0-8.5) had little effect on the process. The requirement for Mg during encystment was probably not due to its deficiency during growth. Encystment was inhibited to varying extents by inhibitors of protein synthesis, tetracycline and chloramphenicol and also by arsenate, arsenite and iodoacetate; sodium fluoride, malonate and 2, 4-dinitrophenol were without marked effect.Addition of glucose and α-ketoglutarate to the replacement medium led to improvement in the encystment response. The presence of glutamate and histidine during encystment led to cell death. Other carbon and nitrogen sources had no effect.During encystment there was an increase in the metabolic activity of the amoebae, as measured by their oxygen consumption. This was accompanied by a decrease of about 40% in cellular dry weight and protein content. Of the other chemical components, there were marked initial increases in the levels of total carbohydrates and pentose which were followed by their depletion during cellulose synthesis.Encystment was completed after about 64 hr when the synthesis of cellulose was complete and the oxygen uptake of the amoebae fell to an immeasurable level.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 24 (1969), S. 0 
    ISSN: 1365-2494
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Two silages of contrasting protein content were compared in a 16-week winter-feeding experiment with 12 Ayrshire cows. One silage contained 8.2% DCP in its DM and the other 15.9% DCP. The silages were fed ad lib. with a supplement of either barley or barley plus groundnut cake. The DM digestibilities of the low and high protein silages were 74 and 67%, respectively, and the calculated S.E.s 56 and 47. Silage and total DM intakes were highest in the treatments containing low protein silage and in those containing groundnut. The mean daily milk yields for the treatments with and without groundnut were 35.4 and 32.s5 Ib (16.1 and 14.8 kg), respectively, with the high-protein silage, and 38.1 and 35.0 Ib (17.3 and 15.9 kg) with the low-protein silage. The S.N.F. contents of the milk were low and averaged 8.26 and 8.34% on the high- and low-protein silage treatments, respectively, and were not affected significantly by the supplements. It is concluded that the low-protein silage was superior to the high-protein silage as a feed for cows, and that the digestibility of the silage DM was a truer indication of quality than protein content.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 22 (1969), S. 0 
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Measurements were made of phosphorus uptake by intact tomato plants from solutions labelled with 32P. The plants were exposed to low water potentials by the addition of mannitol to culture solutions. The amounts of labelled phosphorus in the roots and in the shoots wore determined after a one- or two-hour period. Down to -5.4 atmospheres, the amount of labelled phosphorus in the roots remained constant, hut the amount transported to the shoots was reduced. However, potentials of -10.4 atm reduced the amount of labelled phosphorus in both the root and the shoot. Similar results were obtained when plants were tested immediately after water stress was imposed and when tested after water potentials had been lowered gradually.Plants were treated for one hour at low water potentials and then returned to control solutions (−0.4 atm). For a considerable time, these plants had a much lower phosphorus uptake than plants which had remained continuously at −0.4 atm. These data support the idea that a disturbance in mineral nutrition is partly responsible for reduced growth in plants which experience a moderate water deficit.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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