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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 118 (1994), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Vibrio cholerae belonging to the recently described serogroup 0139, which are responsible for the current cholera epidemics in India and Bangladesh, were shown to express pilus-like structures partially cross-reacting with the toxin-coregulated pilus of V. cholerae strain (0395) belonging to the 01 serogroup and classical biotype. The 0139 pili were composed of 20 kDa subunit proteins which were antigenically related to the 20 kDa pilus protein of another diarrhoeagenic non-01 V. cholerae strain (serogroup 034) isolated earlier. The pili described in this study were found to be involved in the intestinal colonization process and, therefore, may contribute towards the virulence of the 0139 epidemic isolates.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 112 (1993), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A clinical isolate of non-01 V. cholerae(10325) was shown to exhibit higher haemagglutination and intestinal adherence activities in vitro when grown in enriched media, such as trypticase soy broth (TSB) as compared to those of cells grown in a synthetic Tris-buffered or ‘T’-medium. A comparison of their cell-surface protein and lipopolysaccharide profiles suggested the involvement of a 20-kDa protein in the cellular adherence process. An antiserum, raised specifically against the 20-kDa protein, recognised pilus structures on the surface of TSB grown cells. Further studies showed that the pilus was morphologically as well as antigenically distinct from toxin coregulated pilus (TCP) or other types of pili expressed by both 01 and non-01 organisms. Inhibition data established the involvement of the 20-kDa protein in haemagglutination as well as intestinal tissue adherence activities of the parent organism.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 89 (1992), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 80 (1991), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mycelial derived protoplasts of the mushroom, Termitomyces clypeatus, did not regenerate in synthetic growth medium but did regenerate if casein hydrolysate was added to the same medium. Aspartic acid, glutamic acid, histidine, of serine and alanine when added individually had a similar effect on regeneration to that of casein hydrolysate. Krebs cycle acids also supported protoplast regeneration in the presence of glucose. Glucose alone, in the regenerating medium supported osmotically stable germ tube formation from protoplasts while Krebs cycle acid alone could not even change the osmotic fragility of the protoplasts. Electron microscopy of germ tube preparations indicated the presence of an incomplete wall structure when regeneration took place in the presence of glucose alone.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Kyklos 44 (1991), S. 0 
    ISSN: 1467-6435
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Sociology , Economics
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  • 6
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Six monoclonal antibodies (MAbs) were produced against a highly immunogenic fraction derived by the chromatographic separation of the soluble preparation of axenic Entamoeba histolytica (strain NIH:200) trophozoites. Isotype characterization of the six MAbs revealed that four belonged to the IgM class and one each to the IgG1 and the IgG2a subclasses. The immunoreactivity patterns and the specificity of the MAbs with homologous and heterologous antigens were analyzed by the enzyme-linked immunotransfer blot technique and by the enzyme-linked immunosorbent assay. The MAbs reacted intensely with isolates of E. histolytica (strain NIH:200 as well as a local isolate MX1) but showed no reactivity with Entamoeba coli, Iodamoeba butschlii, Endolimax nana, Entamoeba hartmanni, free-living amoeba (Acanthamoeba harticolus) and other enteric parasites. Using the IgG1 MAb as a detecting antibody, a polyclonal-monoclonal antibody-based enzyme-linked immunosorbent assay was developed for the detection of E. histolytica antigens in stool samples of infected patients. The detection limit of the assay was 8 ng of amoebic antigen. This test was found to be specific and sensitive and yielded 100% positive results in cases with amoebiasis but did not react with controls included in the evaluation. The MAb-based enzyme-linked immunosorbent assay developed in this study will be an important test for the diagnosis of E. histolytica in the feces of infected humans; however, the limitation of the test is the inability to discriminate the pathogenic status of the amoeba detected in the stool.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of metamorphic geology 9 (1991), S. 0 
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Abstract Spinel-quartz-cordierite and spinel-quartz are found as relic prograde assemblages in Fe-rich granulites from the Araku area, Eastern Ghats belt, India. Subsequent reactions produced orthopyroxene + sillimanite in the former association and garnet + sillimanite in the latter. The first reaction is univariant in the FMAS system, but is trivariant in the present case because of the presence of Zn and Fe3+ in spinel. The second reaction also has high variance because of Zn and Fe3+, but also because of the presence of Ca in garnet. Thermobarometry shows that the metamorphic conditions were approximately 950° C and 8.5 kbar and the fo2 was near the NNO buffer. In Fe-rich bulk compositions and low-P-high-T conditions of metamorphism, two of the univariant reactions around the invariant point [Sa], namely (Sa, Hy) and (Sa, Cd), change topology due to reverse partitioning of Fe-Mg between coexisting garnet and spinel. An alternative partial petrogenetic grid in the system FMAS is constructed for such conditions and is applied satisfactorily to several sapphirine-free spinel granulites. It is shown that bulk composition (XFe and Zn) exerts greater control on the stability of spinel + quartz than fo2. The effect of the presence of Zn and Fe3+ in spinel on the proposed grid is evaluated. Reaction textures in the Araku spinel granulites can be explained from the petrogenetic grid as due to near-isobaric cooling.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of metamorphic geology 8 (1990), S. 0 
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Using the experimental data on Fe–Mg exchange between orthopyroxene and biotite of Fonarev & Konilov (1986), an orthopyroxene–biotite geothermometer is developed. The thermometer is corrected for mixing of Ti and Al in octahedral sites in biotite and also for non-ideal mixing of Fe and Mg in orthopyroxene. The thermometer is applied to several amphibolite–granulite transition facies and granulite facies rocks and also to mantle xenoliths. It yields consistent results in rocks of widely varying bulk composition, and highly magnesian mantle xenoliths. This thermometer removes the difficulty of estimating temperature in garnet-free rocks in high-grade terrains and also provides independent estimates of temperature in garnet-bearing assemblages.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of metamorphic geology 10 (1992), S. 0 
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: A suite of metapelites, charnockites, calc-silicate rocks, quartzo-feldspathic gneisses and mafic granulites is exposed at Garbham, a part of the Eastern Ghats granulite belt of India. Reaction textures and mineral compositional data have been used to determine the P–T–X evolutionary history of the granulites. In metapelites and charnockites, dehydration melting reactions involving biotite produced quartzofeldspathic segregations during peak metamorphism. However, migration of melt from the site of generation was limited. Subsequent to peak metamorphism at c. 860° C and 8 kbar, the complex evolved through nearly isothermal decompression to 530–650° C and 4–5 kbar. During this phase, coronal garnet grew in the calc-silicates, while garnet in the presence of quartz broke down in charnockite and mafic granulite. Fluid activities during metamorphism were internally buffered in different lithologies in the presence of a melt phase. The P–T path of the granulites at Garbham contrasts sharply with the other parts of the Eastern Ghats granulite belt where the rocks show dominantly near-isobaric cooling subsequent to peak metamorphism.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Bulletin of economic research 44 (1992), S. 0 
    ISSN: 1467-8586
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: The paper develops a mean variance model to characterize the price efficiency at the firm level in Farrell's model and shows how risk aversion may affect this measure. Problems of estimating the structural efficiency at the industry level are also discussed and it is shown that this involves a comparison between efficiency distributions of two or more industries. Two empirical applications for the two efficiency measures are also discussed.
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