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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 71 (1990), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Two derivatives of plasmid pAT153 which arose spontaneously in phosphate-limited continuous cultures of Escherichia coli HB101[pAT153] have been characterised. The smaller plasmid, pLAB446, resulted from a deletion of 446 base pairs of DNA covering the translation start and codon sequence of the tetracycline resistance gene. The other plasmid, pLAB135, differed from pAT153 only by an A:T to G:C transition in the transcribed but untranslated leader region of the tet gene which decreased tet transcription by 84%, possibly due to the formation of a more stable stem-loop structure in the mRNA.
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  • 2
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 580 (1990), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 8 (1994), S. 0 
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Increasing restrictions on sewage sludge disposal routes and the tightening of treatment requirements have led to an escalation of sludge-disposal costs which are out of proportion to other sewage-treatment costs: consequently reducing sludge volume is becoming increasingly cost-effective. Consolidation of anaerobically digested sludge has traditionally been difficult and takes many weeks in secondary digesters or deep storage tanks. Pre-aeration of the sludge using the Aercon process can enable consolidation to take place in days instead of weeks.This paper reports the results of a pilot-scale plant designed to investigate further the mechanism by which aeration speeds up consolidation. It was concluded that the rate of consolidation depended on aeration intensity and cooling of the sludge. Inhibition of anaerobiosis was more important than gas stripping in enhancing consolidation.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Terra nova 4 (1992), S. 0 
    ISSN: 1365-3121
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Biological damage to plants is commonly found from the Devonian but occurs most commonly from the Cretaceous. Damage inflicted on plants whilst they were alive may trigger a pathological response involving the growth of abnormal tissues. Much of the damage is caused by arthropods, particularly insects. Whilst some damage is non-taxon specific, such as simple feeding traces, other damage, such as leaf mines, galls or bark boring, may reveal the co-evolution of host specific taxa and the timing of such interactions. Damaged plants, particularly from the Cretaceous and Tertiary are described and illustrated. The geological history of the evolution of insect-related plant damage is briefly reviewed. Increased variety in the pathological response of plants is seen from the Cretaceous with the evolution of the angiosperms and diversification of numerous insect groups.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 58 (2001), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The ratios of stable isotopes 18O/16O and 13C/12C, in sagittal otolith carbonate from two tropical demersal teleosts, red emperor Lutjanus sebae and Rankin cod Epinephelis multinotatus, from several locations in northern Western Australia, differed between sites. On a broad scale, fish from the four locations, Shark Bay, Ningaloo, Pilbara, and Broome had stable isotope values that were sufficiently different to indicate separate stocks, and it is appropriate to manage these populations of the two species independently in these areas. On a smaller scale, there may be limited mixing of these species between the Pilbara trawl fishery and the trap and line fisheries operating out of Onslow and Broome. Values of stable oxygen isotopes were strongly related to sea surface temperature, although there were some sites in shallow water where low values of stable oxygen isotopes indicated that fish were living in warm water. The use of stable oxygen and carbon isotope values is a valuable, cost effective method of determining the degree of mixing of fish stocks.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 118 (1994), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The angular variation of the median destructive field (MDF) of magnetite particles has been determined by subjecting anisotropic samples carrying an isothermal remanent magnetization (IRM) to static AF demagnetization, with the AF at an angle to the IRM. The alignment was produced by dispersing the magnetite particles in resin in the presence of a magnetic field thus making the sample anisotropic. After tumble demagnetization, an IRM in 80 mT was given to each sample along the alignment axis. 11 different particle sizes were used covering the range 0.7–82 μm. The results for all the samples are remarkably similar in that the MDF is smallest when the AF is applied parallel to the alignment axis and greatest when the AF is perpendicular to the axis. The results are also very similar to those found experimentally for single-domain ‘tape’ particles and indicate that the reversal mechanism even in the smallest particles is not by coherent reversal as in the Stoner-Wohlfarth model. The angular variation of the MDF for all the particle sizes is described quite well by a function derived by Aharoni for single-domain particles. The MDF at θ= 0 is found to be inversely proportional to the square root of the particle size.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 33 (1993), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of five herbicide safeners on preventing maize (Zea mays L.) injury by acetochlor [N-(2-ethyl-6-methyl-phenyl)-N-(ethoxymethyl)-chloroacetamide], their influence on herbicide uptake and metabolism to a glutathione (GSH) conjugate as well as on GSH content and glutathione S-transferase activity (GST) in untreated and herbicide with/without safener-pre-treated 4-day-old seedlings were determined. The safeners studied were: AD-67 (N-di-chloroacetyl-1-oxa-4-azaspiro-4-5-decane), BAS-145138 [1-dichloroacetyl-hexahydro-3,3,8a-trimethyl-pyrrolo(l,2-a)pyrimidin-6(2H)-one], dichlormid (N, A,-diallyl-2,2-dichlo-roacetamide), DKA-24 (N, N2-diallyl-N2dichloroacetylglycineamide) and MG-191 (2-dichloromethyl-2-methyl-1,3-dioxolane). All safeners significantly increased [14C]acetochlor uptake and metabolism rate, maize GSH content and GST activity. Seedlings receiving BAS-145138 pre-treatment metabolized almost 70% of the absorbed [14C]acetochlor within 10 min.Safener-enhanced GST activity was always found to be higher when [14C]acetochlor was used as the substrate compared with CDNB (1-chloro-2,4-dinitrobenzene). Although DKA-24 had a significantly lower influence on both herbicide metabolism and GST activity, it was nearly as effective a safener as BAS-145138, while the others provided no or poor protection to maize from acetochlor injury when they were not incorporated in the soil. Effets d'antidotes sur la toxicité, la pénétration et le métabolisme de I'acétochlore chez le mats etsur l'activité glutathion S-transférase Cinq antidotes d'herbicides diminuaient la toxicité de 1'acétochlore [N-(2-éthyl-6-méthyl-phényl)-N-(éthoxyméthyl)-chloroacétamide] à I'égard du maïs (Zea mays L.). Lew effet sur l'absorption de l'herbicide et sur son métabolisme en un conjugué avec le glutathion (GSH), de même que leur effet sur la teneur en GSH et 1'activité glutathion 5-transférase (GST) ont étéétudiés sur des maïs âgés de 4 jours. Les antidotes étaient: l'AD-67 (N-dichloroacétyl-1-oxa-4-azaspiro-4,5-décane), le BAS-145138 [1 -dichloroacétyl-hexahydro-3,3,8a-triméthyl-pyrrolo(l,2-a) pyrimidine-6(2H)-one], le di-chlormide (N, N-diallyl-2,2-dichloracétamide), le DKA-24 (N, N2-diallyl-N2-dichloracétylgly-cineamide] et le MG-191 (2-dichlorométhyl-2-méthyl-l,3-dioxolane). Tous les antidotes augmentaient de manière significative 1'absorption et le métabolisme de I'acétochlore 14C, ainsi que la teneur du maïs en GSH et son activité GST. Les jeunes plantes prétraitées avec le BAS-145138 métabolisaient en 10 min près de 70% de I'acétochlore 14C absorbé. L'activité GST stimulée par 1'antidote était toujours plus élevée avec I'acétochlore 14C comme substrat qu'avec le CDNB (1-chloro-2,4-dinitrobenzène).Le DKA-24 avait un effet significativement plus faible que le BAS-145138 sur le métabolisme de l'herbicide et sur l'activité GSH, mais son action antidote était presque aussi importante. Les autres produits n'apportaient au maïs qu'une protection faible ou nulle contre l'acétochlore quand ils n'étaient pas incorporés au sol. Wirkung von Safenern auf die Phytotoxizität, Aufnahme und Metabolismus von Acetochlor und die Glutathion-S-Transferase-Aktivität in Mais Die Wirkung von 5 Safenern (AD-67, BAS-145138, Dichlormid, DKA-24 und MG-191; chemische Bezeichnungen s.o.) auf die Phytotoxizität von Acetochlor für Mais (Zea mays L.) und ihr Einfluß auf die Aufnahme des Herbizids und Metabolismus zu einem Glutathion-(GSH-) Konjugat und auf die Glutathion-S-Transferase-(GST-)Aktivität wurde an 4 Tage alten Keimpflanzen untersucht. Durch die Vorbehandlung mit den Safenern wurden die [14C]-Acetochlor-Aufnahme- und -Metabolierungsrate, der GSH-Gehalt und die GST-Ak-tivität signifikant erhöht. Mit BAS-145138 vorbehandelte Keimpflanzen metabolisierten fast 70% des absorbierten [14C]-Acetochlor innerhalb von 10 min. Die durch die Safener erhöhte GST-Aktivität bei [14C]-Acetochlor als Substrat war im Vergleich zu Chlordinitroben-zen immer höher. Obwohl DKA-24 einen signifikant geringeren Einfluß sowohl auf den Herbizidmetabolismus als auch auf die GST-Aktivität hatte, war es fast so wirkungsvoll wie BAS-145138, während die anderen ohne Einarbeitung in den Boden für den Mais keinen oder geringen Schutz gegen Schäden durch Acetochlor boten.
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  • 9
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of aerobic mesophilic microfloral concentration on the isolation of Listeria monocytogenes LM82 was studied in 31 (18 cheeses and 7 noncheese) retail foods having standard plate counts of 101 to 108 colony forming units (CFU)/g. Foods were spiked with L. monocytogenes and enriched at 30°C for 24 h in a selective enrichment broth used in a U.S. Food and Drug Administration method. Inoculum levels for isolation on modified McBride agar ranged from 0.1 to 〉 5 × 103 with a geometric mean value of 5 inoculated CFU/g or 1.4 CFU/g. Pure Enterococcus (Streptococcus) faecalis (0 to 6 × 106 inoculated CFU/mL) in the absence of food matrix had no effect on the enrichment of L. monocytogenes. Ease of isolation of LM82 was independent of the food microflora concentration both generally and in the specific food type of 9 Brie cheeses. Competition, when it occurs, therefore, may be due to specific bacterial competitors rather than bacterial numbers.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Broccoli is well recognized as a source of glucosinolates and their isothiocyanate breakdown products. Glucoraphanin is one of the most abundant glucosinolates present in broccoli and its cognate isothiocyanate is sulphoraphane, a potent inducer of mammalian detoxication (phase 2) enzyme activity and anti-cancer agent. This study was designed to measure: glucosinolate levels in broccoli florets from an array of genotypes grown in several environments; the elevation of a key phase 2 enzyme, quinone reductase, in mammalian cells exposed to floret extracts; and total broccoli head content. There were significant environmental and genotype-by-environment effects on levels of glucoraphanin and quinone reductase induction potential of broccoli heads; however, the effect of genotype was greater than that of environmental factors. The relative rankings among genotypes for glucoraphanin and quinone reductase induction potential changed, when expressed on a per head basis, rather than on a concentration basis. Correlations of trait means in one environment vs. means from a second were stronger for glucoraphanin and quinone reductase induction potential on a per head basis than on a fresh weight concentration basis. Results of this study indicate that development of a broccoli phenotype with a dense head and a high concentration of glucoraphanin to deliver maximum chemoprotective potential (high enzyme induction potential/glucoraphanin content) is a feasible goal.
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