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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 158 (1998), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An extracellular amyloglucosidase (56 kDa) of Termitomyces clypeatus which was accumulated intracellularly in absence of Krebs cycle acids in dextrin medium, remained mostly inside the plasma membrane. The enzyme was localised in mycelial vacuoles as a sucrase-amyloglucosidase aggregate. The intracellular pool contained both the free sucrase and the aggregate, while vacuoles contained only aggregate without any free enzyme. The aggregate, partially purified to PAGE homogeneity, contained amyloglucosidase and sucrase in the ratio of 1:1. A cellular regulation, influenced by the presence of Krebs cycle acids, was indicated to be present at the level of exocytosis of vacuolar enzyme.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 154 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Termitomyces clypeatus secreted a 24-kDa xylanase constitutively in xylan medium, but required a gluconeogenic amino acid or Krebs cycle acid for the secretion of a 56-kDa amyloglucosidase in dextrin medium. Aspartate, glutamate, succinate and fumarate all increased secretion of amyloglucosidase from 50% to 〉90% and enzyme production by 10-fold with little effect on xylanase production. Glutamate or succinate stimulated in vitro release of intracellular amyloglucosidase from washed mycelia in the presence of cycloheximide. Amyloglucosidase accumulated in the absence of glutamate was a high-molecular-mass protein that did not migrate in PAGE. Cellular regulation by the fungus of the secretion of amyloglucosidase is indicated.
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A diarrheogenic strain of non-O1/non-O139 Vibrio cholerae (10325) belonging to serogroup O34 was earlier shown to express a new type of pilus composed of a 20-kDa subunit protein. Amino-terminal sequence data (determined up to 20 amino acid residues) of this protein showed it to be different from the subunit proteins of other known types of pili of V. cholerae. On the other hand, it showed complete homology with the corresponding sequence of a 22-kDa outer membrane protein (OmpW) of V. cholerae. Expression of 10325 pili was favored in AKI rather than in NB medium and at 30°C rather than at 37°C. Further, cultural conditions favoring pilus expression also enhanced autoagglutination and adherence properties of strain 10325. An antiserum to the 20-kDa protein induced passive protection against challenge with the parent organism 10325, but not against V. cholerae O1 strains. Such protection was shown to be mediated by inhibition of intestinal colonization in vivo.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Review of income and wealth 41 (1995), S. 0 
    ISSN: 1475-4991
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: We provide an alternative axiomatization of Sen's (1976) poverty measure. We derive the measure from the general definition of a poverty measure by using a version of Sen's rank order axiom, and a substantially weaker form of his normalization axiom. These two axioms, together with a continuity axiom, lead uniquely to the measure suggested by Sen (1976).
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  • 5
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: A suite of garnet-wollastonite-scapolite-bearing calcsilicate granulites from the Eastern Ghats has been investigated to document the controls of mineral reactions during the metamorphic evolution of the deep continental crust. The rocks studied show heterogeneity in modal mineralogy and phase compositions in millimetre-sized domains. Textural relations, and the compositional plots of the phases, established that the clinopyroxene exerts a strong influence on the formation and composition of garnet in the complex natural system. P-T estimates using the vapour-independent equilibria involving garnet define a near isobaric cooling path from c. 850C at c. 5.5–5.2 kbar. The deduced trajectory tallies well with the terminal segment of the overall retrograde P-T path construed from the associated rocks using well-calibrated thermobarometers. The ubiquitous occurrence of wollastonite and scapolite in the main calcsilicate body suggests low aCO2 during peak metamorphic condition. Fluid compositions constrained from mineral-fluid equilibria of the garnet-bearing assemblages show domainal variations as a function of the compositions of the solid phases, e.g. garnet and clinopyroxene. A quantitative log/CO2-log/O2 diagram has been constructed to depict the stability of the different calcsilicate assemblages as functions of the compositions and the behaviour of these fugitive species. The results of the mineral-fluid equilibria and the quantitative fluid/rock ratio calculations, in conjunction with the topological constraints, imply vapour-deficient meta-morphism in the rocks studied. It is argued that fO2 during peak metamorphism was monitored by the ambient fO2. Subsequently, during retrogression, different domains evolved independently, whereas the fluid composition was controlled by the mineral-fluid equilibria.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Metroeconomica 6 (1954), S. 0 
    ISSN: 1467-999X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Type of Medium: Electronic Resource
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