ISSN:
1574-6968
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
In this communication we report the first successful transformation of Lactobacillus delbrückii ssp. lactis WS97 with plasmid DNA by means of electroporation and describe the optimization of the transformation procedure for this strain. Efficiencies of electroporation varied between 102 to 104 transformants per μg pGK12, depending on the strain from which the DNA was isolated. The application of electroporation in molecular cloning was achieved by using the newly constructed origin screening vector, pAZ8. The replication origins of two cryptic plasmids were cloned. These plasmids were isolated from a thermophilic Lactobacillus strain Lb. delbrückii ssp. lactis WS97 and a mesophilic Lactobacillus strain Lb. casei NCDO151 which are both used in the dairy industry as starter cultures.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1574-6968.1991.tb04444.x
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