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  • 1
    Electronic Resource
    Electronic Resource
    Controllable Crystalline Nano-TiO2 by Homogeneous Hydrolysis with Toluene-p-Sulfonic Acid (2005)
    Oxford, UK : Blackwell Science Inc
    Journal of the American Ceramic Society 88 (2005), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The homogeneous hydrolysis method was proposed to synthesize nano-TiO2 with mixed crystal of anatase and rutile. It is remarkable that the crystal-form-controllable nanosize TiO2 was obtained by the addition of toluene-p-sulfonic acid (TSA). Two processes of the rutile phase formation were proposed. TiO2 was pure anatase crystalline in the absence of TSA. The sample with 0.2 mole ratio of TSA was mixed crystal of anatase and rutile, and characterized that the size of nanocrystalline was 19.5 nm for rutile and 13.5 nm for anatase, the specific surface area was 72.7 m2/g, and the energy of band gap was 2.83 eV. And the mechanism of the development of rutile nano-TiO2 was presented in homogeneous hydrolysis system with TSA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1551-2916.2004.00036.x
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  • 2
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    Increased methylation at differentially methylated region of GNAS in infants born to gestational diabetes (2014)
    BioMed Central
    Details
    Publication Date: 2014-10-03
    Description: Background: Offspring of pregnancy complicated with gestational diabetes (GDM) are at high risk for metabolic diseases. The mechanisms behind the association of intrauterine exposure to GDM and high risk of health problems in later life remain largely unknown. The aim of this study was to clarify the alteration in methylation levels at differentially methylated regions (DMRs) of GNAS and IGF2 in fetuses of GDM women and to explore the possible mechanisms linking maternal GDM with high risk of metabolic diseases in later life of GDM offspring. Methods: The methylation levels were detected in 7 CpG sites of GNAS DMRs and 6 sites of IGF2 DMRs. Methylation levels were significantly higher at sites 4, 5 and 7 of GNAS DMR in GDM compared to normal pregnancy (P?=?0.007, 0.008 and 0.008, respectively). The methylation level at site 4 of GNAS was significantly correlated with the presence of GDM (P?=?0.003), the methylation levels at site 5 and 7 were significantly correlated with the presence of GDM (P?=?0.002 for both) and gestational age (P?=?0.027 for both). There were no significant difference in any sites of IGF2 DMR (P?〉?0.05 for all). Results: Lymphocytes were isolated from umbilical cord blood of infants born to 87 women with GDM and 81 women with normal pregnancy. Genomic DNA was extracted and DNA methylation levels of GNAS and IGF2 DMRs were determined by Massarray quantitative methylation analysis. Conclusions: We concluded maternal GDM-induced hypermethylation at GNAS DMR and this condition may be among the mechanisms associating maternal GDM with increased risk of metabolic diseases in later life of offspring.
    Electronic ISSN: 1471-2350
    Topics: Biology , Medicine
    Published by BioMed Central
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  • 3
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    RNA-Seq derived identification of differential transcription in the chrysanthemum leaf following inoculation with Alternaria tenuissima (2014)
    BioMed Central
    Details
    Publication Date: 2014-01-05
    Description: Background: A major production constraint on the important ornamental species chrysanthemum is black spot which is caused by the necrotrophic fungus Alternaria tenuissima. The molecular basis of host resistance to A. tenuissima has not been studied as yet in any detail. Here, high throughput sequencing was taken to characterize the transcriptomic response of the chrysanthemum leaf to A. tenuissima inoculation. Results: The transcriptomic data was acquired using RNA-Seq technology, based on the Illumina HiSeqTM 2000 platform. Four different libraries derived from two sets of leaves harvested from either inoculated or mock-inoculated plants were characterized. Over seven million clean reads were generated from each library, each corresponding to a coverage of 〉350,000 nt. About 70% of the reads could be mapped to a set of chrysanthemum unigenes. Read frequency was used as a measure of transcript abundance and therefore as an identifier of differential transcription in the four libraries. The differentially transcribed genes identified were involved in photosynthesis, pathogen recognition, reactive oxygen species generation, cell wall modification and phytohormone signalling; in addition, a number of varied transcription factors were identified. A selection of 23 of the genes was transcription-profiled using quantitative RT-PCR to validate the RNA-Seq output. Conclusions: A substantial body of chrysanthemum transcriptomic sequence was generated, which led to a number of insights into the molecular basis of the host response to A. tenuissima infection. Although most of the differentially transcribed genes were up-regulated by the presence of the pathogen, those involved in photosynthesis were down-regulated.
    Electronic ISSN: 1471-2164
    Topics: Biology
    Published by BioMed Central
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  • 4
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    A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum (2014)
    BioMed Central
    Details
    Publication Date: 2014-01-11
    Description: Background: Inorganic phosphate (Pi) is essential for plant growth, and phosphorus deficiency is a main limiting factor in plant development. Its acquisition is largely mediated by Pht1 transporters, a family of plasma membrane-located proteins. Chrysanthemum is one of the most important ornamental plants, its productivity is usually compromised when grown in phosphate deficient soils, but the study of phosphate transporters in chrysanthemum is limited. Results: We described the isolation from chrysanthemum of a homolog of the Phosphate Transporter 1 (PT1) family. Its predicted product is a protein with 12 transmembrane domains, highly homologous with other high affinity plant Pi transporters. Real-time quantitative PCR analysis revealed that the gene was transcribed strongly in the root, weakly in the stem and below the level of detection in the leaf of chrysanthemum plants growing in either sufficient or deficient Pi conditions. Transcript abundance was greatly enhanced in Pi-starved roots. A complementation assay in yeast showed that CmPT1 partially compensated for the absence of phosphate transporter activity in yeast strain MB192. The estimated Km of CmPT1 was 35.2 muM. Under both Pi sufficient and deficient conditions, transgenic plants constitutively expressing CmPT1 grew taller than the non-transformed wild type, produced a greater volume of roots, accumulated more biomass and took up more phosphate. Conclusions: CmPT1 encodes a typical, root-expressed, high affinity phosphate transporter, plays an important role in coping Pi deficiency of chrysanthemum plants.
    Electronic ISSN: 1471-2229
    Topics: Biology
    Published by BioMed Central
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  • 5
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    Genome-wide organization and expression profiling of the R2R3-MYB transcription factor family in pineapple (Ananas comosus) (2017)
    BioMed Central
    Details
    Publication Date: 2017-07-03
    Description: The MYB proteins comprise one of the largest families of plant transcription factors, which are involved in various plant physiological and biochemical processes. Pineapple (Ananas comosus) is one of three most i...
    Electronic ISSN: 1471-2164
    Topics: Biology
    Published by BioMed Central
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