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  • 1
    Publication Date: 2021-03-29
    Description: In the presented case study, ascomycete fungi and green algae on a marble monument were identified by comparisons of the 18S rRNA gene sequences, which were obtained from DNA either from environmental samples or from enrichment cultures. The organisms were found to be responsible for either black or green surface coverings on different areas of the monument surface. Most fungi were related to plant-inhabiting genera, corresponding to a heavy soiling of the marble surface with honeydew. Whereas green algae of the genera Stichococcus, Chloroidium and Apatococcus were found to be dominant in all samples, isolates of two additional genera were recovered only from enrichment cultures. A reference strain of Apatococcus lobatus and an isolate of Prasiolopsis sp. were investigated with respect to putative surface adhesive structures of the cell envelope. The Prasiolopsis cell walls were covered with a thin adhesive exopolysaccharide layer involved in biofilm formation.
    Keywords: Marble monument; Biofilm; Ascomycete fungi; Green algae; Cell wall; Exopolysaccharide ; 551 ; Earth Sciences; Geology
    Language: English
    Type: article , publishedVersion
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  • 2
    Publication Date: 2021-03-29
    Description: In the present study, the diversity of ascomycete fungi was investigated on two wall areas of the “Burg Gleichen”, Thuringia (Germany), made of various types of sandstones, travertine and Grenzdolomit. From a W-exposed, shaded wall area, free-living ascomycetes (mainly “black fungi”) and green algae could be retrieved from sandstone lithologies. Sandstone from an ESE-exposed area was mainly colonized by lichen ascomycetes and the lichen alga Trebouxia. Both areas share a small number of generalist species, related to the ascomycete black fungi Sarcinomyces petricola, Phaeococcomyces chersonesos and Stichococcus mirabilis. Free-living black fungi were isolated and characterized with respect to cell wall morphology and melanin content. A remarkably rigid melanin layer, incorporated in the cell wall of a Cladosporium isolate is presented in detail.
    Keywords: Dimension stone; Biofilm; Ascomycete fungi; Green algae; Cell wall; Melanin ; 551 ; Earth Sciences; Geology
    Language: English
    Type: article , publishedVersion
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