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  • Springer  (500)
  • American Institute of Physics (AIP)
  • Society of Economic Geologists (SEG)
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  • 1
    Electronic Resource
    Electronic Resource
    The suitability of restriction fragment length polymorphisms as genetic markers in maize (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 765-771 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays L ; Restriction fragment length polymorphism (RFLP) ; Genetic mapping ; B-A translocations ; Recombinant inbreds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Strain identification in Zea mays by restriction fragment length polymorphism should be feasible due to the high degree of polymorphism found at many loci. The polymorphism in maize is apparently higher than that currently known for any other organism. Five randomly selected maize inbred lines were examined by Southern filter hybridization with probes of cloned low copy sequences. Typically, several alleles could be distinguished among the inbred lines with any one probe and an appropriately selected restriction enzyme. Despite considerable polymorphism at the DNA level, 16 RFLP markers in three inbred lines of maize were examined for six to 11 generations and found be stable. Mapping of RFLP markers in maize can be accelerated by the use of B-A translocation stocks, which enable localization of a marker to chromosome arm in one generation. The use of recombinant inbred lines in further refinement of the map is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276416
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  • 2
    Electronic Resource
    Electronic Resource
    DNase I hypersensitivity and expression of the Shrunken-1 gene of maize (1987)
    Springer
    Plant molecular biology 8 (1987), S. 251-264 
    Details
    ISSN: 1573-5028
    Keywords: chromatin structure ; DNase I hypersensitivity ; gene expression ; sucrose synthetase ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The local chromatin structure of the Shrunken-1 (Sh) gene of maize was probed by analyzing DNase I hypersensitivity. Sh encodes the gene for sucrose synthetase, a major starch biosynthetic enzyme, which is maximally expressed in the endosperm during seed maturation. In addition to general DNase I sensitivity, specific DNase I hypersensitive sites were identified in endosperm chromatin that mapped near the 5′ end of the Sh gene. The pattern of hypersensitive sites and their relative sensitivity were altered in other non-dormant tissues that produce little or no enzyme. However, some changes in chromatin structure appear to be independent of Sh gene expression and may reflect general alterations associated with plant development. The chromatin structure of several sh mutations, induced by Ds controlling element insertions, was also analyzed. Although the insertions perturbed expression of the gene, there were no notable effects on local chromatin structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015033
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  • 3
    Electronic Resource
    Electronic Resource
    The Promoter Region of the Carbamoyl-Phosphate Synthetase III Gene of Squalus acanthias (1996)
    Springer
    Journal of molecular evolution 43 (1996), S. 602-609 
    Details
    ISSN: 1432-1432
    Keywords: Key words:Squalus acanthias— Carbamoyl-phosphate synthetase — Promoter —Rana catesbeiana— TATA box — TACAAA — C/EBP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Carbamoyl-phosphate synthetase III (CPSase III) of Squalus acanthias (spiny dogfish) is a nuclear-encoded mitochondrial enzyme that catalyzes glutamine-dependent formation of carbamoyl phosphate for urea synthesis. In this paper we report the results of cloning a 10-kb segment of genomic DNA which includes the region flanking the 5′ end of the spiny dogfish CPSase III gene. A total of 1,295 base pairs of sequence straddling the start codon was obtained. Primer extension experiments revealed that the transcription start site is the G located 114 residues upstream of the translation start codon ATG. The first exon has 240 base pairs, including the 5′ untranslated region, the coding sequence for the signal peptide (38 amino acids), and the four N-terminal amino acids of the mature enzyme. The boundary of the first exon and the first intron of the CPSase III gene is concordant with that of rat and frog (Rana catesbeiana) CPSase I, which have been suggested to have evolved from CPSase III. The putative TATA box sequence, TACAAA, is located at position −31 with an uncommonly found C at the third position. Two C/EBP binding site sequences, ATTCTGCAAG (−405 to −397) and GTGCAGTAAG (−168 to −160), were identified in the promoter region, which suggests that spiny dogfish CPSase III might be subjected to transactivation of transcription by C/EBP-related proteins, as has been reported for rat CPSase I. The preparation and binding of a recombinant RcC/EBP-1 protein (the R. catesbeiana homolog of the mammalian C/EBPα) to the two spiny dogfish C/EBP binding sequences are described. Two putative heat-shock binding elements were also identified in the promoter region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02202108
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  • 4
    Electronic Resource
    Electronic Resource
    Intragenomic DNA sequence homologies in the chicken and other members of the classAves: DNA re-association under reduced stringency conditions (1980)
    Springer
    Journal of molecular evolution 15 (1980), S. 291-307 
    Details
    ISSN: 1432-1432
    Keywords: Avian genome evolution ; Intragenomic DNA sequence homology Reduced-stringency DNA reassociation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have investigated the intragenomic DNA sequence homologies of twelve species of birds representing five orders, and emphasizing Galliformes. This study differs in two important ways from the classical approaches taken in constructing and evaluating phylogenies based on DNA sequence similarities. Comparisons are made on the basis of sequence homologieswithin genomes of related birds, rather than between genomes. DNA is reassociated at 50°C in 0.5M phosphate buffer; these conditions allow formation and detection of duplexes containing more mismatch than would normally be permitted using more stringent conditions, affording an opportunity to observe more ancient sequence homologies. Thermal stability profiles of DNA duplexes formed under these conditions are the basis of comparison; three general patterns were observed. This approach emphasizes differences in sequence composition between genomes while the more traditional method of intergenomic tracer DNA hybridization at higher stringency emphasizes sequence similarities. No correlation was found between taxonomic position and intragenomic sequence composition, either within or between lineages. The thermal stability profiles of DNA duplexes formed within avian genomes did not reflect the biological similarities inferred from morphology, karyotype, and studies of interspecific hybridization. While all of the differences observed could have occurred over geological time, it was surprising that the genomes of the domestic chicken and the Red Jungle Fowl (Gallus gallus) differ in their sequence compositions. It appears that amplification/reduction events and/or positional changes occur rather often during evolution of a lineage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01733136
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  • 5
    Electronic Resource
    Electronic Resource
    The promoter region of the carbamoyl-phosphate synthetase III gene ofSqualus acanthias (1996)
    Springer
    Journal of molecular evolution 43 (1996), S. 602-609 
    Details
    ISSN: 1432-1432
    Keywords: Squalus acanthias ; Carbamoyl-phosphate synthetase ; Promoter ; Rana catesbeiana ; TATA box ; TACAAA ; C/EBP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carbamoyl-phosphate synthetase III (CPSase III) ofSqualus acanthias (spiny dogfish) is a nuclear-encoded mitochondrial enzyme that catalyzes glutamine-dependent formation of carbamoyl phosphate for urea synthesis. In this paper we report the results of cloning a 10-kb segment of genomic DNA which includes the region flanking the 5′ end of the spiny dogfish CPSase III gene. A total of 1,295 base pairs of sequence straddling the start codon was obtained. Primer extension experiments revealed that the transcription start site is the G located 114 residues upstream of the translation start codon ATG. The first exon has 240 base pairs, including the 5′ untranslated region, the coding sequence for the signal peptide (38 amino acids), and the four N-terminal amino acids of the mature enzyme. The boundary of the first exon and the first intron of the CPSase III gene is concordant with that of rat and frog (Rana catesbeiana) CPSase I, which have been suggested to have evolved from CPSase III. The putative TATA box sequence, TACAAA, is located at position −31 with an uncommonly found C at the third position. Two C/EBP binding site sequences, ATTCTGCAAG (−405 to −397) and GTGCAGTAAG (−168 to −160), were identified in the promoter region, which suggests that spiny dogfish CPSase III might be subjected to transactivation of transcription by C/EBP-related proteins, as has been reported for rat CPSase I. The preparation and binding of a recombinant RcC/EBP-1 protein (theR. catesbeiana homolog of the mammalian C/EBPα) to the two spiny dogfish C/EBP binding sequences are described. Two putative heatshock binding elements were also identified in the promoter region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02202108
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrafast dynamics of holes in GaAs probed by two-color femtosecond spectroscopy (1998)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 73 (1998), S. 64-66 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Ultrafast relaxation dynamics of light and heavy holes in GaAs following femtosecond valence-to-conduction-band excitation are measured by probing the light- and heavy-to-split-off hole transitions at different midinfrared wavelengths using the recently developed broadly tunable femtosecond optical parametric oscillator. The initial relaxation times are less than 75 fs, and a spectral hole-burning effect is seen. The results suggest that carrier–carrier and optical-phonon scattering, in particular, polar optical-phonon scattering, are the primary processes leading to the initial redistribution of heavy and light holes. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.121724
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  • 7
    Electronic Resource
    Electronic Resource
    Structural and magnetic properties of stage 2 CocMn1−cCl2-graphite intercalation compounds : The 5th Joint MMM−Intermag Conference (1991)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 70 (1991), S. 6098-6100 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Stage 2 CocMn1−cCl2-graphite intercalation compounds (0≤c≤1) approximate two-dimensional site-random XY spin systems with a competition between ferromagnetic [J(Co–Co) and J(Co–Mn)], and antiferromagnetic exchange interactions [J(Mn–Mn)]. The magnetic phase diagram and critical behavior of these compounds are studied by means of ac and dc magnetic susceptibility. For c≥ (R18)0.45 the phase transition occurs between the paramagnetic phase and the ferromagnetic phase. The critical exponents of γ and β do not change with dilution of Mn atoms: γ=2.31 and β=0.085 for c=0.90.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.350058
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  • 8
    Electronic Resource
    Electronic Resource
    Magnetic properties of random-mixture graphite intercalation compounds (1990)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 67 (1990), S. 5749-5751 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The magnetic properties of a quasi-two-dimensional mixed ferromagnetic and antiferromagnetic system, stage-2 CocMn1−cCl2 -graphite intercalation compounds (GICs), have been studied by dc magnetic susceptibility measurements. The Curie–Weiss temperature determined as a function of Co concentration indicates that the intraplanar exchange interaction between Co2+ and Mn2+ is ferromagnetic and that J(Co-Mn)=1.3 [J(Co-Co)||J(Mn-Mn)||]1/2. With decreasing Co concentration c, the transition temperature tends to decrease to zero as c approaches 0.5 (the percolation concentration for the triangular lattice sites), but is not reduced to zero even below c=0.5. There may be a possibility of a spin-glass phase for 0≤c〈0.4 arising from the competition between J(Mn-Mn), J(Co-Mn), and J(Mn-Mn).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.345951
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  • 9
    Electronic Resource
    Electronic Resource
    Rayleigh–Bénard convection in liquid metal layers under the influence of a vertical magnetic field (2001)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 13 (2001), S. 3247-3257 
    Details
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The influence of a vertical magnetic field on the integral heat transfer and the temporal dynamics of liquid metal Rayleigh–Bénard convection is studied in an experiment using a small Prandtl number (Pr(approximate)0.02) sodium potassium alloy Na22Kr78 as a test fluid. The test section is a rectangular box of large aspect ratio 20 : 10 : 1 that covers a parameter range of Rayleigh numbers, 103〈Ra〈105, and Chandrasekhar numbers, 0〈Q〈1.44×104. The integral heat transfer across the layer is evaluated from the measured temperatures at the upper and the lower boundary and the applied heat flux. Local, time-dependent temperatures are obtained from a four-element temperature probe placed in the middle of the liquid metal layer. The noncoplanar arrangement of the thermocouples enables the evaluation of the time-dependent temperature gradient vector that allows us to estimate the local isotropy properties of the time-dependent flow. From the damping effect of Joule dissipation, the convective heat transport decreases monotonically with increasing Chandrasekhar numbers. Fluctuations of the temperature field are damped significantly by the magnetic field. However, this effect is selective with respect to frequency. Long period fluctuations are strongly damped whereas short period fluctuations are less damped or may even be amplified. The observations show that significant convective heat transport is practically always associated with time-dependent flow. The fluctuating part of the local temperature gradient confirms the horizontal isotropy of the velocity field; no predominant orientation of time-dependent flow structures is established either with or without a magnetic field. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1404385
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  • 10
    Electronic Resource
    Electronic Resource
    28 ps recovery time in an InGaAsP/InGaAsP multiple-quantum-well saturable absorber employing carrier sweepout (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 90 (2001), S. 3566-3569 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We describe fast recovery at a wavelength of 1550 nm in a multiple-quantum-well (MQW) saturable absorber with InGaAsP quaternary wells and barriers using electric-field-induced carrier sweepout. The MQW SA is integrated with a distributed Bragg reflector in a p-type–intrinsic–n-type structure. Pump–probe measurements show that the recovery time can be reduced from 〉900 to 28 ps by application of a 151 kV/cm sweepout field. We measure the dependence of the recovery time on the energy of the saturating pulse and explain our results in terms of carrier dynamics in the sweepout field. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1399032
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