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  • American Institute of Physics (AIP)  (39)
  • Blackwell Publishing Ltd  (17)
  • MDPI Publishing  (14)
  • 1
    Electronic Resource
    Electronic Resource
    Quantitative Estimation of Dark Muscle Content in the Mackerel Meat Paste and its Products Using Antisera Against Myosin Light Chains (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 66 (2001), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Based on the high level of extractability of myosin subunits (light chains), even after prolonged heat treatment of muscle, a new method to evaluate the dark muscle content in the fish meat and products of mackerel is proposed. Tissue-specific rabbit antisera with myosin light chains (A1 from ordinary muscle and D1 from dark muscle) from mackerel Scomber japonicus were obtained. Mackerel meat paste (surimi) was dissolved in 8 M urea containing 1% SDS, and diffused on agar plates containing antiserum against A1 or D1 by single radial immunodiffusion (SRID). The results obtained showed that the area of halos formed in the plates was quite proportional to the content of dark muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2001.tb15205.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mycobacterium bovis bacillus Calmette-Guérin infection promotes SOCS induction and inhibits IFN-γ-stimulated JAK/STAT signaling in J774 macrophages (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 39 (2003), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The resurgence in mycobacterial infection worldwide has led to renewed attention to the pathogenesis of Mycobacterium species. Although interferon-γ (IFN-γ) is a principal mediator of macrophage activation, macrophages infected with Mycobacterium are poor in response at the cytokine. However, the molecular mechanisms underlying mycobacterial infection remain unclear. The purpose of this study was to elucidate the mechanism of the poor response to IFN-γ in mycobacterial infection. Our data clearly demonstrate that this is due to induction of suppressor of cytokine signal (SOCS) negative regulators of IFN-γ signal transduction that closely correlates with the inhibition of JAK/STAT signaling and gene expression stimulated by IFN-γ. Mycobacterium bovis bacillus Calmette-Guérin infection induces the production of SOCS-1 and SOCS-3 in murine J774 macrophages. The level of SOCS-1 mRNA increased 1 h and reached a maximum 3 h after the addition of the bacteria. SOCS-3 mRNA expression appeared as early as 1 h after the infection. We also observed that trehalose 6,6′-dimycolate/cord factor, a major component of the Mycobacterium tuberculosis cell wall, induces expression of SOCS and inhibits IFN-γ-stimulated phosphorylation of STAT1 extensively in the cells. The results in this study suggest that a molecular mechanism of mycobacterial infection affects the unresponsiveness to IFN-γ in the subsequent growth and spread of macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0928-8244(03)00231-1
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  • 3
    Electronic Resource
    Electronic Resource
    Hydrogen cyanide laser interferometer of the JFT-2M tokamak and feedback stabilization of the beat frequency using a microcomputer (1986)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 57 (1986), S. 1290-1295 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A hydrogen cyanide laser interferometer with three vertical chords was installed in the JFT-2M tokamak for electron density measurement. The optical arrangment of the interferometer is a Mach–Zehnder type and its structure is mechanically independent of the JFT-2M machine in order to suppress the mechanical vibration. Two HCN lasers are employed for direct reading of the chord averaged electron density by means of beat modulation method. The beat frequency between two HCN lasers is stabilized at a constant frequency by a feedback circuit using a microcomputer. A combination of short dielectric waveguides and TPX lenses is employed to compensate the refraction and divergence of the laser beam. It was shown experimentally that the interferometric measurement of the electron density was scarcely affected by the mechanical vibration of the interferometer and the refraction of the probing laser beam up to n¯e=6×1019 m−3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1138590
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  • 4
    Electronic Resource
    Electronic Resource
    Study of inhomogeneity of supercritical water by small-angle x-ray scattering (2000)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 112 (2000), S. 4203-4211 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Small-angle x-ray scattering (SAXS) experiments using synchrotron radiation were carried out for supercritical water along isotherms at the temperatures T=660.0, 661.5, 663.0, 677.0, and 687.5 K, from a gaslike density region to a liquidlike one, including an intermediate density region. The high-temperature and high-pressure sample holder for SAXS measurements suitable for supercritical water was redesigned for more precise measurements. The curves illustrating the density dependence of density fluctuations and correlation lengths show a slight shift of the maximum from critical isochore. The deviations become larger with increasing temperature. The results for the density fluctuations and correlation lengths for supercritical water are compared with those for supercritical CO2 and CF3H at T/Tc=1.02 and 1.06. The comparison allows us to draw the conclusion that the behavior in the long-range inhomogeneity of water in the supercritical state is in discord with the ordinary behaviors for other molecular substances. Density fluctuations in water are also compared with those of Ar and Hg calculated thermodynamically by use of the equations of state. The correlation of the symmetry between the contour of density fluctuations and the vapor–liquid coexistence curve is discussed. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.480965
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  • 5
    Electronic Resource
    Electronic Resource
    Correlation time of density fluctuation for supercritical ethylene studied by dynamic light scattering (2002)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 116 (2002), S. 4985-4992 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Time dependence of density fluctuation for neat supercritical ethylene (C2H4) is investigated by dynamic light scattering at various densities in isothermal conditions of reduced temperatures Tr=T/Tc=1.02, 1.04, and 1.06. Time correlations of the density fluctuation for all thermodynamic states decay as single exponential functions with the time constant of submicrosecond. Critical slowing down of diffusive motions is observed in the time domain, and the correlation time of the density fluctuation becomes maximum at the extension of the gas–liquid coexistence curve on the P–T phase diagram. It is revealed that the time dependence of the density fluctuation just corresponds to the magnitude of static density fluctuation obtained by small-angle x-ray scattering measurements. By measuring correlation times as a function of scattering angle, the critical slowing down is thermodynamically discussed. It is elucidated experimentally that the critical slowing down of diffusive motions considerably correlates to the increase of specific heat capacity, the decrease of the thermal diffusivity, and rotational relaxation time in the supercritical state of the neat fluid. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1452112
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of three individual glucosyltransferases produced by Streptococcus mutans using monoclonal antibodies (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 145 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We previously established murine hybridomas producing a monoclonal antibody monospecific against three glucosyl-transferases (I, SI and S) of Streptococcus mutans which contribute to dental caries formation. Here, we developed a new immunochemical technique (cross-dot system) with which individual levels of glucosyltransferases expressed by S. mutans can be evaluated. We also examined glucosyltransferase production and in vitro artificial plaque formation by a reference strain and several clinical isolates of S. mutans. The findings indicate that the levels of glucosyltransferases produced greatly vary with the cells and the culture medium, and that the cells producing high levels of both glucosyltransferase-SI and glucosyltransferase-I enzymes may possess high in vitro artificial plaque forming ability. We suggest that the cross-dot system will be useful for estimating the cariogenic potential of S. mutans isolates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08611.x
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  • 7
    Electronic Resource
    Electronic Resource
    Penicillin-binding proteins in Mycobacterium smegmatis (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 25 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The penicillin-binding proteins (PBPs) of Mycobacterium smegmatis were studied. Five PBPs ranging in Mr from approx. 114000 to 25000 were detected in the cytoplasmic membrane. The affinities of the PBPs for selected β-lactam antibiotics were determined. Most of the antibiotics bound to PBPs 3 and 4 at low concentrations. A penicillin-susceptible mutant and a cefmetazole-resistant mutant were isolated by selection in vitro. The PBPs of these mutants were identical to those of the parent strain. The affinity of cefmetazole for the individual PBPs was identical in each mutant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01477.x
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  • 8
    Electronic Resource
    Electronic Resource
    Heat-Induced Tendering of Turban Shell (Batillus cornutus) Muscle (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The foot, opercular and visceral muscles were excised from the turban shell Batillus cornutus, and their heat-induced changes in toughness and ultrastructure were compared. When heated up to 30°C, the toughness of foot muscle decreased in the upper and middle regions, but increased in the lower region. The toughness of these three regions decreased at temperatures between 50-70°C, where collagen might be denatured. Some changes in toughness were also observed in the opercular muscle by heating up to 30°C, whereas the toughness of visceral muscle remained unchanged over a wide range of temperature. Transmission electron microscopy showed that the intrinsic structure of collagen fibrils in each muscle was lost at 60°C, with partial swelling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb12994.x
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  • 9
    Electronic Resource
    Electronic Resource
    Autoxidation of Bluefin Tuna Myoglobin Associated with Freezing and Thawing (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 52 (1987), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bluefin tuna oxymyoglobin (oxyMb) solutions were frozen either quickly at −80°C or slowly at −2O°C, thawed, and the metmyoglobin (metMb) to total myoglobin (Mb) ratio (metMb%) determined. When quick freezing was applied, autoxidation was clearly pH-dependent, with a minimum (metMb%, 15–40) at around pH 5.9–6.3. When slowly frozen and thawed, on the other hand, tuna Mb generally showed an extremely high metMb%. Freezing and thawing partially insolubilized tuna Mb, depending upon pH. The Mb showed the minimum ratio of insolubilization at pH 6–6.3 again. Insolubilization of tuna Mb was markedly accelerated by NaCl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1987.tb06681.x
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  • 10
    Electronic Resource
    Electronic Resource
    Annealing effects for implanted Y-Ba-Cu-O superconductors (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 936-938 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Annealing effects of Y-Ba-Cu-O superconductor thin films implanted by 200-keV Ne+ have been investigated. Transition temperature (Tc) end points for 0, 1×1014, 1×1015, and 1×1016 ions/cm2 doses are 75, 71, 62, and 16 K, respectively. The film implanted at 1×1017 ions/cm2 dose indicates nonsuperconductors. The c-lattice constant increases were observed for the implanted films. It is confirmed that the superconducting characteristics for film, which is implanted at 1×1016 ions/cm2 dose, are recovered by annealing in O2 atmosphere at 940 °C for 4 h. Moreover, microcrystal growth caused by annealing the implanted film was observed on the surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.341897
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