Publication Date:
1987-05-15
Description:
A better understanding of the molecular mechanism of protein biosynthesis depends on the availability of a reliable model for the ribosome particle. The application of a diffraction technique, namely, three-dimensional image reconstruction from two-dimensional sheets of the large ribosomal subunits of Bacillus stearothermophilus at a resolution of 30 angstroms is described. The resulting three-dimensional model shows at least four projecting arms, arranged radially near the presumed interface with the 30S subunit. The projecting arms are positioned around a cleft, which turns into a tunnel with a length of 100 to 120 angstroms and a diameter of up to 25 angstroms. This tunnel spans the particle and may provide the path taken by the nascent polypeptide chain.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yonath, A -- Leonard, K R -- Wittmann, H G -- GM34360/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1987 May 15;236(4803):813-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3576200" target="_blank"〉PubMed〈/a〉
Keywords:
Geobacillus stearothermophilus/*ultrastructure
;
Microscopy, Electron
;
Models, Molecular
;
Ribosomes/*ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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