Publication Date:
2003-07-12
Description:
We report the use of genetically engineered cells in a pathogen identification sensor. This sensor uses B lymphocytes that have been engineered to emit light within seconds of exposure to specific bacteria and viruses. We demonstrated rapid screening of relevant samples and identification of a variety of pathogens at very low levels. Because of its speed, sensitivity, and specificity, this pathogen identification technology could prove useful for medical diagnostics, biowarfare defense, food- and water-quality monitoring, and other applications.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rider, Todd H -- Petrovick, Martha S -- Nargi, Frances E -- Harper, James D -- Schwoebel, Eric D -- Mathews, Richard H -- Blanchard, David J -- Bortolin, Laura T -- Young, Albert M -- Chen, Jianzhu -- Hollis, Mark A -- New York, N.Y. -- Science. 2003 Jul 11;301(5630):213-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. thor@ll.mit.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12855808" target="_blank"〉PubMed〈/a〉
Keywords:
Aequorin/biosynthesis
;
Antibodies, Bacterial/immunology
;
Antibodies, Viral/immunology
;
*B-Lymphocytes/immunology
;
Bacillus anthracis/immunology/isolation & purification
;
Bacteria/immunology/*isolation & purification
;
*Bacteriological Techniques
;
*Biosensing Techniques
;
Cell Line
;
Colony Count, Microbial
;
Encephalitis Virus, Venezuelan Equine/immunology/isolation & purification
;
Escherichia coli O157/immunology/isolation & purification
;
Foot-and-Mouth Disease Virus/immunology/isolation & purification
;
Immunoglobulin Variable Region/immunology
;
Light
;
Receptors, Antigen, B-Cell/immunology
;
Sensitivity and Specificity
;
Time Factors
;
Transfection
;
Viruses/immunology/*isolation & purification
;
Yersinia pestis/immunology/isolation & purification
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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