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  • 1
    Publication Date: 2000-10-13
    Description: Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells. FLAIR revealed precise spatial control of growth factor-induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kraynov, V S -- Chamberlain, C -- Bokoch, G M -- Schwartz, M A -- Slabaugh, S -- Hahn, K M -- AG15430/AG/NIA NIH HHS/ -- GM39434/GM/NIGMS NIH HHS/ -- R01 GM-57464/GM/NIGMS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 2000 Oct 13;290(5490):333-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11030651" target="_blank"〉PubMed〈/a〉
    Keywords: 3T3 Cells ; Actins/metabolism ; Animals ; Biosensing Techniques ; Blood ; Cell Membrane/*enzymology/physiology/ultrastructure ; *Cell Movement ; Cell Nucleus/*enzymology ; Cell Polarity ; Enzyme Activation ; Fluorescence ; Guanosine Triphosphate/*metabolism ; Mice ; Nuclear Envelope/enzymology ; Platelet-Derived Growth Factor/pharmacology ; Recombinant Fusion Proteins/metabolism ; Spectrometry, Fluorescence ; rac1 GTP-Binding Protein/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1998-06-26
    Description: The small eccentricity of Neptune may be a direct consequence of apsidal wave interaction with the trans-Neptune population of debris called the Kuiper belt. The Kuiper belt is subject to resonant perturbations from Neptune, so that the transport of angular momentum by density waves can result in orbital evolution of Neptune as well as changes in the structure of the Kuiper belt. In particular, for a belt eroded out to the vicinity of Neptune's 2:1 resonance at about 48 astronomical units, Neptune's eccentricity can damp to its current value over the age of the solar system if the belt contains slightly more than an earth mass of material out to about 75 astronomical units.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ward -- Hahn -- New York, N.Y. -- Science. 1998 Jun 26;280(5372):2104-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉W. R. Ward, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 USA. J. M. Hahn, Lunar and Planetary Institute, 3600 Bay Area Boulevard, Houston, TX 77058 USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9641913" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2004-09-14
    Description: Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nalbant, Perihan -- Hodgson, Louis -- Kraynov, Vadim -- Toutchkine, Alexei -- Hahn, Klaus M -- GM57464/GM/NIGMS NIH HHS/ -- GM64346/GM/NIGMS NIH HHS/ -- R01 GM057464/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Sep 10;305(5690):1615-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7365, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15361624" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/metabolism ; Algorithms ; Animals ; *Biosensing Techniques ; Cell Adhesion ; Cell Line ; Cell Membrane/*metabolism ; Cell Polarity ; Cell Surface Extensions/metabolism/ultrastructure ; Endothelial Cells/metabolism/ultrastructure ; Fibroblasts ; Fluorescence ; Fluorescent Dyes/chemistry/metabolism ; Green Fluorescent Proteins ; Humans ; Luminescent Proteins ; Mice ; Microtubules/metabolism ; Neutrophil Activation ; Neutrophils/*metabolism ; Proteins/chemistry/metabolism ; Pseudopodia/metabolism ; Pyrimidinones/metabolism ; Sensitivity and Specificity ; Wiskott-Aldrich Syndrome Protein ; cdc42 GTP-Binding Protein/*metabolism ; rho GTP-Binding Proteins/metabolism ; trans-Golgi Network/*metabolism/ultrastructure
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 2019
    Description: 〈p〉Cytopenias are an important clinical problem associated with inflammatory disease and infection. We show that specialized phagocytes that internalize red blood cells develop in Toll-like receptor 7 (TLR7)–driven inflammation. TLR7 signaling caused the development of inflammatory hemophagocytes (iHPCs), which resemble splenic red pulp macrophages but are a distinct population derived from Ly6C〈sup〉hi〈/sup〉 monocytes. iHPCs were responsible for anemia and thrombocytopenia in TLR7-overexpressing mice, which have a macrophage activation syndrome (MAS)–like disease. Interferon regulatory factor 5 (IRF5), associated with MAS, participated in TLR7-driven iHPC differentiation. We also found iHPCs during experimental malarial anemia, in which they required endosomal TLR and MyD88 signaling for differentiation. Our findings uncover a mechanism by which TLR7 and TLR9 specify monocyte fate and identify a specialized population of phagocytes responsible for anemia and thrombocytopenia associated with inflammation and infection.〈/p〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
    Publication Date: 2011-10-29
    Description: Asteroid 21 Lutetia was approached by the Rosetta spacecraft on 10 July 2010. The additional Doppler shift of the spacecraft radio signals imposed by 21 Lutetia's gravitational perturbation on the flyby trajectory were used to determine the mass of the asteroid. Calibrating and correcting for all Doppler contributions not associated with Lutetia, a least-squares fit to the residual frequency observations from 4 hours before to 6 hours after closest approach yields a mass of (1.700 +/- 0.017) x 10(18) kilograms. Using the volume model of Lutetia determined by the Rosetta Optical, Spectroscopic, and Infrared Remote Imaging System (OSIRIS) camera, the bulk density, an important parameter for clues to its composition and interior, is (3.4 +/- 0.3) x 10(3) kilograms per cubic meter.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Patzold, M -- Andert, T P -- Asmar, S W -- Anderson, J D -- Barriot, J-P -- Bird, M K -- Hausler, B -- Hahn, M -- Tellmann, S -- Sierks, H -- Lamy, P -- Weiss, B P -- New York, N.Y. -- Science. 2011 Oct 28;334(6055):491-2. doi: 10.1126/science.1209389.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Rheinisches Institut fur Umweltforschung, Abteilung Planetenforschung, an der Universitat zu Koln, 50931 Cologne, Germany. martin.paetzold@uni-koeln.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22034429" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
    Publication Date: 2015-10-17
    Description: The Pluto system was recently explored by NASA's New Horizons spacecraft, making closest approach on 14 July 2015. Pluto's surface displays diverse landforms, terrain ages, albedos, colors, and composition gradients. Evidence is found for a water-ice crust, geologically young surface units, surface ice convection, wind streaks, volatile transport, and glacial flow. Pluto's atmosphere is highly extended, with trace hydrocarbons, a global haze layer, and a surface pressure near 10 microbars. Pluto's diverse surface geology and long-term activity raise fundamental questions about how small planets remain active many billions of years after formation. Pluto's large moon Charon displays tectonics and evidence for a heterogeneous crustal composition; its north pole displays puzzling dark terrain. Small satellites Hydra and Nix have higher albedos than expected.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stern, S A -- Bagenal, F -- Ennico, K -- Gladstone, G R -- Grundy, W M -- McKinnon, W B -- Moore, J M -- Olkin, C B -- Spencer, J R -- Weaver, H A -- Young, L A -- Andert, T -- Andrews, J -- Banks, M -- Bauer, B -- Bauman, J -- Barnouin, O S -- Bedini, P -- Beisser, K -- Beyer, R A -- Bhaskaran, S -- Binzel, R P -- Birath, E -- Bird, M -- Bogan, D J -- Bowman, A -- Bray, V J -- Brozovic, M -- Bryan, C -- Buckley, M R -- Buie, M W -- Buratti, B J -- Bushman, S S -- Calloway, A -- Carcich, B -- Cheng, A F -- Conard, S -- Conrad, C A -- Cook, J C -- Cruikshank, D P -- Custodio, O S -- Dalle Ore, C M -- Deboy, C -- Dischner, Z J B -- Dumont, P -- Earle, A M -- Elliott, H A -- Ercol, J -- Ernst, C M -- Finley, T -- Flanigan, S H -- Fountain, G -- Freeze, M J -- Greathouse, T -- Green, J L -- Guo, Y -- Hahn, M -- Hamilton, D P -- Hamilton, S A -- Hanley, J -- Harch, A -- Hart, H M -- Hersman, C B -- Hill, A -- Hill, M E -- Hinson, D P -- Holdridge, M E -- Horanyi, M -- Howard, A D -- Howett, C J A -- Jackman, C -- Jacobson, R A -- Jennings, D E -- Kammer, J A -- Kang, H K -- Kaufmann, D E -- Kollmann, P -- Krimigis, S M -- Kusnierkiewicz, D -- Lauer, T R -- Lee, J E -- Lindstrom, K L -- Linscott, I R -- Lisse, C M -- Lunsford, A W -- Mallder, V A -- Martin, N -- McComas, D J -- McNutt, R L Jr -- Mehoke, D -- Mehoke, T -- Melin, E D -- Mutchler, M -- Nelson, D -- Nimmo, F -- Nunez, J I -- Ocampo, A -- Owen, W M -- Paetzold, M -- Page, B -- Parker, A H -- Parker, J W -- Pelletier, F -- Peterson, J -- Pinkine, N -- Piquette, M -- Porter, S B -- Protopapa, S -- Redfern, J -- Reitsema, H J -- Reuter, D C -- Roberts, J H -- Robbins, S J -- Rogers, G -- Rose, D -- Runyon, K -- Retherford, K D -- Ryschkewitsch, M G -- Schenk, P -- Schindhelm, E -- Sepan, B -- Showalter, M R -- Singer, K N -- Soluri, M -- Stanbridge, D -- Steffl, A J -- Strobel, D F -- Stryk, T -- Summers, M E -- Szalay, J R -- Tapley, M -- Taylor, A -- Taylor, H -- Throop, H B -- Tsang, C C C -- Tyler, G L -- Umurhan, O M -- Verbiscer, A J -- Versteeg, M H -- Vincent, M -- Webbert, R -- Weidner, S -- Weigle, G E 2nd -- White, O L -- Whittenburg, K -- Williams, B G -- Williams, K -- Williams, S -- Woods, W W -- Zangari, A M -- Zirnstein, E -- New York, N.Y. -- Science. 2015 Oct 16;350(6258):aad1815. doi: 10.1126/science.aad1815.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Southwest Research Institute, Boulder, CO 80302, USA. astern@boulder.swri.edu. ; Laboratory for Atmospheric and Space Physics, University of Colorado, Boulder, CO 80303, USA. ; National Aeronautics and Space Administration (NASA) Ames Research Center, Space Science Division, Moffett Field, CA 94035, USA. ; Southwest Research Institute, San Antonio, TX 28510, USA. ; Lowell Observatory, Flagstaff, AZ 86001, USA. ; Department of Earth and Planetary Sciences, Washington University, St. Louis, MO 63130, USA. ; Southwest Research Institute, Boulder, CO 80302, USA. ; Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA. ; Universitat der Bundeswehr Munchen, Neubiberg 85577, Germany. ; Planetary Science Institute, Tucson, AZ 85719, USA. ; KinetX Aerospace, Tempe, AZ 85284, USA. ; NASA Jet Propulsion Laboratory, La Canada Flintridge, CA 91011, USA. ; Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ; University of Bonn, Bonn D-53113, Germany. ; NASA Headquarters (retired), Washington, DC 20546, USA. ; University of Arizona, Tucson, AZ 85721, USA. ; Cornell University, Ithaca, NY 14853, USA. ; NASA Headquarters, Washington, DC 20546, USA. ; Rheinisches Institut fur Umweltforschung an der Universitat zu Koln, Cologne 50931, Germany. ; Department of Astronomy, University of Maryland, College Park, MD 20742, USA. ; Search for Extraterrestrial Intelligence Institute, Mountain View, CA 94043, USA. ; Department of Environmental Sciences, University of Virginia, Charlottesville, VA 22904, USA. ; NASA Goddard Space Flight Center, Greenbelt, MD 20771, USA. ; National Optical Astronomy Observatory, Tucson, AZ 26732, USA. ; NASA Marshall Space Flight Center, Huntsville, AL 35812, USA. ; Stanford University, Stanford, CA 94305, USA. ; Space Telescope Science Institute, Baltimore, MD 21218, USA. ; University of California, Santa Cruz, CA 95064, USA. ; Lunar and Planetary Institute, Houston, TX 77058, USA. ; Michael Soluri Photography, New York, NY 10014, USA. ; Johns Hopkins University, Baltimore, MD 21218, USA. ; Roane State Community College, Jamestown, TN 38556, USA. ; George Mason University, Fairfax, VA 22030, USA. ; Department of Astronomy, University of Virginia, Charlottesville, VA 22904, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26472913" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
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  • 7
    Publication Date: 2019
    Description: 〈p〉The characteristics of DNA methylation changes that occur during neurogenesis in vivo remain unknown. We used whole-genome bisulfite sequencing to quantitate DNA cytosine modifications in differentiating neurons and their progenitors isolated from mouse brain at the peak of embryonic neurogenesis. Localized DNA hypomethylation was much more common than hypermethylation and often occurred at putative enhancers within genes that were upregulated in neurons and encoded proteins crucial for neuronal differentiation. The hypomethylated regions strongly overlapped with mapped binding sites of the key neuronal transcription factor NEUROD2. The 5-methylcytosine oxidase ten-eleven translocation 2 (TET2) interacted with NEUROD2, and its reaction product 5-hydroxymethylcytosine accumulated at the demethylated regions. NEUROD2-targeted differentially methylated regions retained higher methylation levels in 〈i〉Neurod2〈/i〉 knockout mice, and inducible expression of NEUROD2 caused TET2-associated demethylation at its in vivo binding sites. The data suggest that the reorganization of DNA methylation in developing neurons involves NEUROD2 and TET2-mediated DNA demethylation. 〈/p〉
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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