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  • 1
    Publication Date: 2019
    Description: 〈p〉Fucosylated glycoconjugates are involved in a variety of physiological and pathological processes. However, economical production of fucosylated drugs and prebiotic supplements has been hampered by the poor catalytic efficiency of fucosyltransferases. Here, we developed a fluorescence-activated cell sorting system that enables the ultrahigh-throughput screening (〉10〈sup〉7〈/sup〉 mutants/hour) of such enzymes and designed a companion strategy to assess the screening performance of the system. After three rounds of directed evolution, a mutant M32 of the α1,3-FucT from 〈i〉Helicobacter pylori〈/i〉 was identified with 6- and 14-fold increases in catalytic efficiency (〈i〉k〈/i〉〈sub〉cat〈/sub〉/〈i〉K〈/i〉〈sub〉m〈/sub〉) for the synthesis of Lewis x and 3'-fucosyllactose, respectively. The structure of the M32 mutant revealed that the S45F mutation generates a clamp-like structure that appears to improve binding of the galactopyranose ring of the acceptor substrate. Moreover, molecular dynamic simulations reveal that helix α5, is more mobile in the M32 mutant, possibly explaining its high fucosylation activity.〈/p〉
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 2
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2019
    Description: 〈p〉Most membrane proteins exist in complexes that carry out critical cellular functions and exhibit rich dynamics. The bacterial flagellar motor, a large membrane-spanning ion-driven rotary motor that propels the bacteria to swim, provides a canonical example. Rotation of the motor is driven by multiple torque-generating units (stators). Turnover of the stators has been shown previously, demonstrating the exchange of stator units between the motor and a membrane pool. But the details of the turnover kinetics remain unclear. Here, we directly measured the kinetics of stator turnover in individual motors via analysis of a large dataset of long-term high-resolution recordings of motor speed at high load. We found that the dwell time distribution of the stator units exhibits a multi-exponential shape, suggesting the existence of a hidden state in the turnover of the stators.〈/p〉
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 3
    Publication Date: 2019
    Description: 〈p〉An important reasons for drug relapse is the retrieval of drug withdrawal memory induced by conditioned context. Previous studies have suggested that the basolateral amygdala (BLA) plays an important role in conditioned context–induced retrieval of morphine withdrawal memory. However, the downstream neuronal circuits of the activation of the BLA in conditioned context–induced retrieval of morphine withdrawal memory remain unknown. Using retrograde labeling, immunohistochemical, and optogenetic approaches, we found that, although BLA neurons projecting to the prelimbic cortex (PrL) played an important role in conditioned context–induced retrieval of morphine withdrawal memory, they do not exhibit increased expression of the neuronal plasticity marker Arc. However, when PrL neurons activated by the BLA send feedback signals to the BLA, a neuronal-related process is induced in other BLA neurons that do not project to the PrL, a finding that is relevant to conditioned context–induced retrieval of morphine withdrawal memory.〈/p〉
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 4
    Publication Date: 1999-06-26
    Description: The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors, but only when GRIP1 or SRC-1a was coexpressed. Thus, CARM1 functions as a secondary coactivator through its association with p160 coactivators. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduced both methyltransferase and coactivator activities. Thus, coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, D -- Ma, H -- Hong, H -- Koh, S S -- Huang, S M -- Schurter, B T -- Aswad, D W -- Stallcup, M R -- AG00093/AG/NIA NIH HHS/ -- DK43093/DK/NIDDK NIH HHS/ -- NS17269/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1999 Jun 25;284(5423):2174-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology HMR 301, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10381882" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Cell Line ; Histone Acetyltransferases ; Histones/metabolism ; Methylation ; Mice ; Molecular Sequence Data ; Mutation ; Nuclear Receptor Coactivator 1 ; Nuclear Receptor Coactivator 2 ; Nuclear Receptor Coactivator 3 ; Protein-Arginine N-Methyltransferases/chemistry/genetics/*metabolism ; Receptors, Androgen/metabolism ; Receptors, Estrogen/metabolism ; Receptors, Thyroid Hormone/metabolism ; Recombinant Fusion Proteins/metabolism ; Signal Transduction ; Trans-Activators/*metabolism ; Transcription Factors/metabolism ; *Transcriptional Activation ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
    Publication Date: 2018-10-05
    Description: Biodiversity experiments have shown that species loss reduces ecosystem functioning in grassland. To test whether this result can be extrapolated to forests, the main contributors to terrestrial primary productivity, requires large-scale experiments. We manipulated tree species richness by planting more than 150,000 trees in plots with 1 to 16 species. Simulating multiple extinction scenarios, we found that richness strongly increased stand-level productivity. After 8 years, 16-species mixtures had accumulated over twice the amount of carbon found in average monocultures and similar amounts as those of two commercial monocultures. Species richness effects were strongly associated with functional and phylogenetic diversity. A shrub addition treatment reduced tree productivity, but this reduction was smaller at high shrub species richness. Our results encourage multispecies afforestation strategies to restore biodiversity and mitigate climate change.
    Keywords: Ecology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
    Publication Date: 2011-03-12
    Description: The products of photoreactions of conjugated organic molecules may be allowed by selection rules but not observed in solution reactions because of unfavorable reaction geometries. We have used defect sites in self-assembled alkanethiolate monolayers on gold surfaces to direct geometrically unfavorable photochemical reactions between individual organic molecules. High conductivity and stochastic switching of anthracene-terminated phenylethynylthiolates within alkanethiolate monolayers, as well as in situ photochemical transformations, have been observed and distinguished with the scanning tunneling microscope (STM). Ultraviolet light absorbed during imaging increases the apparent heights of excited molecules in STM images, a direct manifestation of probing electronically excited states.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Moonhee -- Hohman, J Nathan -- Cao, Yang -- Houk, Kendall N -- Ma, Hong -- Jen, Alex K-Y -- Weiss, Paul S -- New York, N.Y. -- Science. 2011 Mar 11;331(6022):1312-5. doi: 10.1126/science.1200830.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉California NanoSystems Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21393542" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
    Publication Date: 2014-05-09
    Description: The efficient use of natural gas will require catalysts that can activate the first C-H bond of methane while suppressing complete dehydrogenation and avoiding overoxidation. We report that single iron sites embedded in a silica matrix enable direct, nonoxidative conversion of methane, exclusively to ethylene and aromatics. The reaction is initiated by catalytic generation of methyl radicals, followed by a series of gas-phase reactions. The absence of adjacent iron sites prevents catalytic C-C coupling, further oligomerization, and hence, coke deposition. At 1363 kelvin, methane conversion reached a maximum at 48.1% and ethylene selectivity peaked at 48.4%, whereas the total hydrocarbon selectivity exceeded 99%, representing an atom-economical transformation process of methane. The lattice-confined single iron sites delivered stable performance, with no deactivation observed during a 60-hour test.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Guo, Xiaoguang -- Fang, Guangzong -- Li, Gang -- Ma, Hao -- Fan, Hongjun -- Yu, Liang -- Ma, Chao -- Wu, Xing -- Deng, Dehui -- Wei, Mingming -- Tan, Dali -- Si, Rui -- Zhang, Shuo -- Li, Jianqi -- Sun, Litao -- Tang, Zichao -- Pan, Xiulian -- Bao, Xinhe -- New York, N.Y. -- Science. 2014 May 9;344(6184):616-9. doi: 10.1126/science.1253150.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24812398" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
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  • 8
    Publication Date: 2014-03-29
    Description: Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATalpha allele on synIII. The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033833/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033833/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Annaluru, Narayana -- Muller, Heloise -- Mitchell, Leslie A -- Ramalingam, Sivaprakash -- Stracquadanio, Giovanni -- Richardson, Sarah M -- Dymond, Jessica S -- Kuang, Zheng -- Scheifele, Lisa Z -- Cooper, Eric M -- Cai, Yizhi -- Zeller, Karen -- Agmon, Neta -- Han, Jeffrey S -- Hadjithomas, Michalis -- Tullman, Jennifer -- Caravelli, Katrina -- Cirelli, Kimberly -- Guo, Zheyuan -- London, Viktoriya -- Yeluru, Apurva -- Murugan, Sindurathy -- Kandavelou, Karthikeyan -- Agier, Nicolas -- Fischer, Gilles -- Yang, Kun -- Martin, J Andrew -- Bilgel, Murat -- Bohutski, Pavlo -- Boulier, Kristin M -- Capaldo, Brian J -- Chang, Joy -- Charoen, Kristie -- Choi, Woo Jin -- Deng, Peter -- DiCarlo, James E -- Doong, Judy -- Dunn, Jessilyn -- Feinberg, Jason I -- Fernandez, Christopher -- Floria, Charlotte E -- Gladowski, David -- Hadidi, Pasha -- Ishizuka, Isabel -- Jabbari, Javaneh -- Lau, Calvin Y L -- Lee, Pablo A -- Li, Sean -- Lin, Denise -- Linder, Matthias E -- Ling, Jonathan -- Liu, Jaime -- Liu, Jonathan -- London, Mariya -- Ma, Henry -- Mao, Jessica -- McDade, Jessica E -- McMillan, Alexandra -- Moore, Aaron M -- Oh, Won Chan -- Ouyang, Yu -- Patel, Ruchi -- Paul, Marina -- Paulsen, Laura C -- Qiu, Judy -- Rhee, Alex -- Rubashkin, Matthew G -- Soh, Ina Y -- Sotuyo, Nathaniel E -- Srinivas, Venkatesh -- Suarez, Allison -- Wong, Andy -- Wong, Remus -- Xie, Wei Rose -- Xu, Yijie -- Yu, Allen T -- Koszul, Romain -- Bader, Joel S -- Boeke, Jef D -- Chandrasegaran, Srinivasan -- 092076/Wellcome Trust/United Kingdom -- GM077291/GM/NIGMS NIH HHS/ -- R01 GM077291/GM/NIGMS NIH HHS/ -- R01 GM090192/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2014 Apr 4;344(6179):55-8. doi: 10.1126/science.1249252. Epub 2014 Mar 27.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Environmental Health Sciences, Johns Hopkins University (JHU) School of Public Health, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24674868" target="_blank"〉PubMed〈/a〉
    Keywords: Base Sequence ; *Chromosomes, Fungal/genetics/metabolism ; DNA, Fungal/genetics ; Genes, Fungal ; Genetic Fitness ; Genome, Fungal ; Genomic Instability ; Introns ; Molecular Sequence Data ; Mutation ; Polymerase Chain Reaction ; RNA, Fungal/genetics ; RNA, Transfer/genetics ; Saccharomyces cerevisiae/cytology/*genetics/physiology ; Sequence Analysis, DNA ; Sequence Deletion ; Synthetic Biology/*methods ; Transformation, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 2014-08-26
    Description: Helium nanodroplets are considered ideal model systems to explore quantum hydrodynamics in self-contained, isolated superfluids. However, exploring the dynamic properties of individual droplets is experimentally challenging. In this work, we used single-shot femtosecond x-ray coherent diffractive imaging to investigate the rotation of single, isolated superfluid helium-4 droplets containing ~10(8) to 10(11) atoms. The formation of quantum vortex lattices inside the droplets is confirmed by observing characteristic Bragg patterns from xenon clusters trapped in the vortex cores. The vortex densities are up to five orders of magnitude larger than those observed in bulk liquid helium. The droplets exhibit large centrifugal deformations but retain axially symmetric shapes at angular velocities well beyond the stability range of viscous classical droplets.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gomez, Luis F -- Ferguson, Ken R -- Cryan, James P -- Bacellar, Camila -- Tanyag, Rico Mayro P -- Jones, Curtis -- Schorb, Sebastian -- Anielski, Denis -- Belkacem, Ali -- Bernando, Charles -- Boll, Rebecca -- Bozek, John -- Carron, Sebastian -- Chen, Gang -- Delmas, Tjark -- Englert, Lars -- Epp, Sascha W -- Erk, Benjamin -- Foucar, Lutz -- Hartmann, Robert -- Hexemer, Alexander -- Huth, Martin -- Kwok, Justin -- Leone, Stephen R -- Ma, Jonathan H S -- Maia, Filipe R N C -- Malmerberg, Erik -- Marchesini, Stefano -- Neumark, Daniel M -- Poon, Billy -- Prell, James -- Rolles, Daniel -- Rudek, Benedikt -- Rudenko, Artem -- Seifrid, Martin -- Siefermann, Katrin R -- Sturm, Felix P -- Swiggers, Michele -- Ullrich, Joachim -- Weise, Fabian -- Zwart, Petrus -- Bostedt, Christoph -- Gessner, Oliver -- Vilesov, Andrey F -- New York, N.Y. -- Science. 2014 Aug 22;345(6199):906-9. doi: 10.1126/science.1252395.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, University of Southern California (USC), Los Angeles, CA 90089, USA. ; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA. ; Ultrafast X-ray Science Laboratory, Chemical Sciences Division, Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA 94720, USA. ; Ultrafast X-ray Science Laboratory, Chemical Sciences Division, Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA 94720, USA. Department of Chemistry, University of California Berkeley, Berkeley, CA 94720, USA. ; Max-Planck-Institut fur Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany. Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany. ; Department of Physics and Astronomy, USC, Los Angeles, CA 90089, USA. ; Max-Planck-Institut fur Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany. Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany. Deutsches Elektronen-Synchrotron (DESY), Notkestrasse 85, 22607 Hamburg, Germany. ; Advanced Light Source, LBNL, Berkeley, CA 94720, USA. ; CFEL, DESY, Notkestrasse 85, 22607 Hamburg, Germany. ; Max-Planck-Institut fur Extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany. ; Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany. Max-Planck-Institut fur Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany. ; PNSensor GmbH, Otto-Hahn-Ring 6, 81739 Munchen, Germany. ; Mork Family Department of Chemical Engineering and Materials Science, USC, Los Angeles, CA 90089, USA. ; Ultrafast X-ray Science Laboratory, Chemical Sciences Division, Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA 94720, USA. Department of Chemistry, University of California Berkeley, Berkeley, CA 94720, USA. Department of Physics, University of California Berkeley, Berkeley, CA 94720, USA. ; Ultrafast X-ray Science Laboratory, Chemical Sciences Division, Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA 94720, USA. Department of Physics, The Chinese University of Hong Kong, Hong Kong, China. ; National Energy Research Scientific Computing Center, LBNL, Berkeley, CA 94720, USA. ; Physical Biosciences Division, LBNL, Berkeley, CA 94720, USA. Department of Plant and Microbial Biology, University of Calfornia Berkeley, Berkeley, CA 94720, USA. ; Advanced Light Source, LBNL, Berkeley, CA 94720, USA. Department of Physics, University of California Davis, Davis, CA 95616, USA. ; Physical Biosciences Division, LBNL, Berkeley, CA 94720, USA. ; Department of Chemistry, University of California Berkeley, Berkeley, CA 94720, USA. ; Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany. Deutsches Elektronen-Synchrotron (DESY), Notkestrasse 85, 22607 Hamburg, Germany. Max-Planck-Institut fur Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany. ; Max-Planck-Institut fur Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany. Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany. James R. Macdonald Laboratory, Department of Physics, Kansas State University, Manhattan, KS 66506, USA. ; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA. PULSE Institute, Stanford University and SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA. bostedt@slac.stanford.edu ogessner@lbl.gov vilesov@usc.edu. ; Ultrafast X-ray Science Laboratory, Chemical Sciences Division, Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA 94720, USA. bostedt@slac.stanford.edu ogessner@lbl.gov vilesov@usc.edu. ; Department of Chemistry, University of Southern California (USC), Los Angeles, CA 90089, USA. Department of Physics and Astronomy, USC, Los Angeles, CA 90089, USA. bostedt@slac.stanford.edu ogessner@lbl.gov vilesov@usc.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25146284" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
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  • 10
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2016-02-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ma, Eric H -- Jones, Russell G -- New York, N.Y. -- Science. 2016 Feb 12;351(6274):670-1. doi: 10.1126/science.aaf1929.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Goodman Cancer Research Centre, Department of Physiology, McGill University, Montreal, QC, H3A 1A3, Canada. Department of Physiology, McGill University, Montreal, QC, H3G 1Y6, Canada. ; Goodman Cancer Research Centre, Department of Physiology, McGill University, Montreal, QC, H3A 1A3, Canada. Department of Physiology, McGill University, Montreal, QC, H3G 1Y6, Canada. russell.jones@mcgill.ca.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26912848" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Humans ; Mitochondria/*metabolism ; Multiprotein Complexes/*metabolism ; Purines/*biosynthesis/*metabolism ; TOR Serine-Threonine Kinases/*metabolism ; Tetrahydrofolates/*metabolism
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    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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