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  • 1
    Publication Date: 2000-04-15
    Description: We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fritz, J -- Baller, M K -- Lang, H P -- Rothuizen, H -- Vettiger, P -- Meyer, E -- Guntherodt, H -- Gerber, C -- Gimzewski, J K -- New York, N.Y. -- Science. 2000 Apr 14;288(5464):316-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉IBM Research, Zurich Research Laboratory, Saumerstrasse 4, CH-8803 Ruschlikon, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10764640" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibody Specificity ; Base Pair Mismatch ; Base Pairing ; Chemistry, Physical ; Goats ; Gold/*chemistry ; Hydrogen Bonding ; Immunoglobulin Constant Regions/*chemistry ; Ligands ; *Nucleic Acid Hybridization ; Oligodeoxyribonucleotides/*chemistry ; Physicochemical Phenomena ; Rabbits ; Silicon/*chemistry ; Staphylococcal Protein A/*chemistry ; Static Electricity ; Stress, Mechanical ; Thionucleotides/chemistry
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  • 2
    Publication Date: 2001-02-07
    Description: Long sediment cores recovered from the deep portions of Lake Titicaca are used to reconstruct the precipitation history of tropical South America for the past 25,000 years. Lake Titicaca was a deep, fresh, and continuously overflowing lake during the last glacial stage, from before 25,000 to 15,000 calibrated years before the present (cal yr B.P.), signifying that during the last glacial maximum (LGM), the Altiplano of Bolivia and Peru and much of the Amazon basin were wetter than today. The LGM in this part of the Andes is dated at 21,000 cal yr B.P., approximately coincident with the global LGM. Maximum aridity and lowest lake level occurred in the early and middle Holocene (8000 to 5500 cal yr B.P.) during a time of low summer insolation. Today, rising levels of Lake Titicaca and wet conditions in Amazonia are correlated with anomalously cold sea-surface temperatures in the northern equatorial Atlantic. Likewise, during the deglacial and Holocene periods, there were several millennial-scale wet phases on the Altiplano and in Amazonia that coincided with anomalously cold periods in the equatorial and high-latitude North Atlantic, such as the Younger Dryas.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Baker, P A -- Seltzer, G O -- Fritz, S C -- Dunbar, R B -- Grove, M J -- Tapia, P M -- Cross, S L -- Rowe, H D -- Broda, J P -- New York, N.Y. -- Science. 2001 Jan 26;291(5504):640-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Duke University, Division of Earth and Ocean Sciences, Durham, NC 27708, USA. pbaker@geo.duke.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11158674" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atmosphere ; Bolivia ; Diatoms ; *Fresh Water ; *Geologic Sediments ; Peru ; Plankton ; *Rain ; Temperature ; Time ; *Tropical Climate
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  • 3
    Publication Date: 2002-06-01
    Description: Glaciation in the humid tropical Andes is a sensitive indicator of mean annual temperature. Here, we present sedimentological data from lakes beyond the glacial limit in the tropical Andes indicating that deglaciation from the Last Glacial Maximum led substantial warming at high northern latitudes. Deglaciation from glacial maximum positions at Lake Titicaca, Peru/Bolivia (16 degrees S), and Lake Junin, Peru (11 degrees S), occurred 22,000 to 19,500 calendar years before the present, several thousand years before the Bolling-Allerod warming of the Northern Hemisphere and deglaciation of the Sierra Nevada, United States (36.5 degrees to 38 degrees N). The tropical Andes deglaciated while climatic conditions remained regionally wet, which reflects the dominant control of mean annual temperature on tropical glaciation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seltzer, G O -- Rodbell, D T -- Baker, P A -- Fritz, S C -- Tapia, P M -- Rowe, H D -- Dunbar, R B -- New York, N.Y. -- Science. 2002 May 31;296(5573):1685-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Earth Sciences, Syracuse University, 204 Heroy Geology Laboratory, Syracuse, NY 13244, USA. goseltze@syr.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12040193" target="_blank"〉PubMed〈/a〉
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  • 4
    Publication Date: 1998-11-30
    Description: HOX genes specify cell fate in the anterior-posterior axis of animal embryos. Invertebrate chordates have one HOX cluster, but mammals have four, suggesting that cluster duplication facilitated the evolution of vertebrate body plans. This report shows that zebrafish have seven hox clusters. Phylogenetic analysis and genetic mapping suggest a chromosome doubling event, probably by whole genome duplication, after the divergence of ray-finned and lobe-finned fishes but before the teleost radiation. Thus, teleosts, the most species-rich group of vertebrates, appear to have more copies of these developmental regulatory genes than do mammals, despite less complexity in the anterior-posterior axis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Amores, A -- Force, A -- Yan, Y L -- Joly, L -- Amemiya, C -- Fritz, A -- Ho, R K -- Langeland, J -- Prince, V -- Wang, Y L -- Westerfield, M -- Ekker, M -- Postlethwait, J H -- P01HD22486/HD/NICHD NIH HHS/ -- R01RR10715/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1998 Nov 27;282(5394):1711-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9831563" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Chromosome Mapping ; Chromosomes/genetics ; *Evolution, Molecular ; Gene Duplication ; *Genes, Homeobox ; *Genome ; Models, Genetic ; *Multigene Family ; Phylogeny ; Pseudogenes ; Zebrafish/*genetics
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  • 5
    Publication Date: 1991-09-27
    Description: At Venus the Energetic Particles Detector (EPD) on the Galileo spacecraft measured the differential energy spectra and angular distributions of ions 〉22 kiloelectron volts (keV) and electrons 〉 15 keV in energy. The only time particles were observed by EPD was in a series of episodic events [0546 to 0638 universal time (UT)] near closest approach (0559:03 UT). Angular distributions were highly anisotropic, ordered by the magnetic field, and showed ions arriving from the hemisphere containing Venus and its bow shock. The spectra showed a power law form with intensities observed into the 120- to 280-keV range. Comparisons with model bow shock calculations show that these energetic ions are associated with the venusian foreshock-bow shock region. Shock-drift acceleration in the venusian bow shock seems the most likely process responsible for the observed ions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Williams, D J -- McEntire, R W -- Krimigis, S M -- Roelof, E C -- Jaskulek, S -- Tossman, B -- Wilken, B -- Studemann, W -- Armstrong, T P -- Fritz, T A -- Lanzerotti, L J -- Roederer, J G -- New York, N.Y. -- Science. 1991 Sep 27;253(5027):1525-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17784094" target="_blank"〉PubMed〈/a〉
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  • 6
    Publication Date: 1992-09-25
    Description: Since its invention a few years ago, the atomic force microscope has become one of the most widely used near-field microscopes. Surfaces of hard sample are imaged routinely with atomic resolution. Soft samples, however, remain challenging. An overview is presented on the application of atomic force microscopy to organic samples ranging from thin ordered films at molecular resolution to living cells. Fundamental mechanisms of the image formation are discussed, and novel imaging modes are introduced that exploit different aspects of the tip-sample interaction for local measurements of the micromechanical properties of the sample. As examples, images of Langmuir-Blodgett films, which map the local viscoelasticity as well as the friction coefficient, are presented.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Radmacher, M -- Tillamnn, R W -- Fritz, M -- Gaub, H E -- New York, N.Y. -- Science. 1992 Sep 25;257(5078):1900-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Physikdepartment, Technische Universitat Munchen, 8046 Garching, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1411505" target="_blank"〉PubMed〈/a〉
    Keywords: Actin Cytoskeleton/ultrastructure ; Amino Acids ; Blood Platelets/cytology ; Crystallography ; Elasticity ; Humans ; Microscopy/*instrumentation ; Platelet Activation ; Proteins/ultrastructure ; Viscosity
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  • 7
    Publication Date: 2007-10-13
    Description: Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the approximately 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875087/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875087/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Merchant, Sabeeha S -- Prochnik, Simon E -- Vallon, Olivier -- Harris, Elizabeth H -- Karpowicz, Steven J -- Witman, George B -- Terry, Astrid -- Salamov, Asaf -- Fritz-Laylin, Lillian K -- Marechal-Drouard, Laurence -- Marshall, Wallace F -- Qu, Liang-Hu -- Nelson, David R -- Sanderfoot, Anton A -- Spalding, Martin H -- Kapitonov, Vladimir V -- Ren, Qinghu -- Ferris, Patrick -- Lindquist, Erika -- Shapiro, Harris -- Lucas, Susan M -- Grimwood, Jane -- Schmutz, Jeremy -- Cardol, Pierre -- Cerutti, Heriberto -- Chanfreau, Guillaume -- Chen, Chun-Long -- Cognat, Valerie -- Croft, Martin T -- Dent, Rachel -- Dutcher, Susan -- Fernandez, Emilio -- Fukuzawa, Hideya -- Gonzalez-Ballester, David -- Gonzalez-Halphen, Diego -- Hallmann, Armin -- Hanikenne, Marc -- Hippler, Michael -- Inwood, William -- Jabbari, Kamel -- Kalanon, Ming -- Kuras, Richard -- Lefebvre, Paul A -- Lemaire, Stephane D -- Lobanov, Alexey V -- Lohr, Martin -- Manuell, Andrea -- Meier, Iris -- Mets, Laurens -- Mittag, Maria -- Mittelmeier, Telsa -- Moroney, James V -- Moseley, Jeffrey -- Napoli, Carolyn -- Nedelcu, Aurora M -- Niyogi, Krishna -- Novoselov, Sergey V -- Paulsen, Ian T -- Pazour, Greg -- Purton, Saul -- Ral, Jean-Philippe -- Riano-Pachon, Diego Mauricio -- Riekhof, Wayne -- Rymarquis, Linda -- Schroda, Michael -- Stern, David -- Umen, James -- Willows, Robert -- Wilson, Nedra -- Zimmer, Sara Lana -- Allmer, Jens -- Balk, Janneke -- Bisova, Katerina -- Chen, Chong-Jian -- Elias, Marek -- Gendler, Karla -- Hauser, Charles -- Lamb, Mary Rose -- Ledford, Heidi -- Long, Joanne C -- Minagawa, Jun -- Page, M Dudley -- Pan, Junmin -- Pootakham, Wirulda -- Roje, Sanja -- Rose, Annkatrin -- Stahlberg, Eric -- Terauchi, Aimee M -- Yang, Pinfen -- Ball, Steven -- Bowler, Chris -- Dieckmann, Carol L -- Gladyshev, Vadim N -- Green, Pamela -- Jorgensen, Richard -- Mayfield, Stephen -- Mueller-Roeber, Bernd -- Rajamani, Sathish -- Sayre, Richard T -- Brokstein, Peter -- Dubchak, Inna -- Goodstein, David -- Hornick, Leila -- Huang, Y Wayne -- Jhaveri, Jinal -- Luo, Yigong -- Martinez, Diego -- Ngau, Wing Chi Abby -- Otillar, Bobby -- Poliakov, Alexander -- Porter, Aaron -- Szajkowski, Lukasz -- Werner, Gregory -- Zhou, Kemin -- Grigoriev, Igor V -- Rokhsar, Daniel S -- Grossman, Arthur R -- GM07185/GM/NIGMS NIH HHS/ -- GM42143/GM/NIGMS NIH HHS/ -- R01 GM032843/GM/NIGMS NIH HHS/ -- R01 GM042143/GM/NIGMS NIH HHS/ -- R01 GM042143-09/GM/NIGMS NIH HHS/ -- R01 GM060992/GM/NIGMS NIH HHS/ -- R01 GM062915-06/GM/NIGMS NIH HHS/ -- R37 GM030626/GM/NIGMS NIH HHS/ -- R37 GM042143/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2007 Oct 12;318(5848):245-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17932292" target="_blank"〉PubMed〈/a〉
    Keywords: Algal Proteins/*genetics/*physiology ; Animals ; *Biological Evolution ; Chlamydomonas reinhardtii/*genetics/physiology ; Chloroplasts/metabolism ; Computational Biology ; DNA, Algal/genetics ; Flagella/metabolism ; Genes ; *Genome ; Genomics ; Membrane Transport Proteins/genetics/physiology ; Molecular Sequence Data ; Multigene Family ; Photosynthesis/genetics ; Phylogeny ; Plants/genetics ; Proteome ; Sequence Analysis, DNA
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  • 8
    Publication Date: 2007-02-03
    Description: Intense femtosecond laser excitation can produce transient states of matter that would otherwise be inaccessible to laboratory investigation. At high excitation densities, the interatomic forces that bind solids and determine many of their properties can be substantially altered. Here, we present the detailed mapping of the carrier density-dependent interatomic potential of bismuth approaching a solid-solid phase transition. Our experiments combine stroboscopic techniques that use a high-brightness linear electron accelerator-based x-ray source with pulse-by-pulse timing reconstruction for femtosecond resolution, allowing quantitative characterization of the interatomic potential energy surface of the highly excited solid.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fritz, D M -- Reis, D A -- Adams, B -- Akre, R A -- Arthur, J -- Blome, C -- Bucksbaum, P H -- Cavalieri, A L -- Engemann, S -- Fahy, S -- Falcone, R W -- Fuoss, P H -- Gaffney, K J -- George, M J -- Hajdu, J -- Hertlein, M P -- Hillyard, P B -- Horn-von Hoegen, M -- Kammler, M -- Kaspar, J -- Kienberger, R -- Krejcik, P -- Lee, S H -- Lindenberg, A M -- McFarland, B -- Meyer, D -- Montagne, T -- Murray, E D -- Nelson, A J -- Nicoul, M -- Pahl, R -- Rudati, J -- Schlarb, H -- Siddons, D P -- Sokolowski-Tinten, K -- Tschentscher, Th -- von der Linde, D -- Hastings, J B -- New York, N.Y. -- Science. 2007 Feb 2;315(5812):633-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Frontiers in Optical Coherent and Ultrafast Science (FOCUS) Center, Departments of Physics and Applied Physics Program, University of Michigan, Ann Arbor, MI 48109, USA. dmfritz@slac.stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17272718" target="_blank"〉PubMed〈/a〉
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  • 9
    Publication Date: 2010-05-08
    Description: Neandertals, the closest evolutionary relatives of present-day humans, lived in large parts of Europe and western Asia before disappearing 30,000 years ago. We present a draft sequence of the Neandertal genome composed of more than 4 billion nucleotides from three individuals. Comparisons of the Neandertal genome to the genomes of five present-day humans from different parts of the world identify a number of genomic regions that may have been affected by positive selection in ancestral modern humans, including genes involved in metabolism and in cognitive and skeletal development. We show that Neandertals shared more genetic variants with present-day humans in Eurasia than with present-day humans in sub-Saharan Africa, suggesting that gene flow from Neandertals into the ancestors of non-Africans occurred before the divergence of Eurasian groups from each other.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Green, Richard E -- Krause, Johannes -- Briggs, Adrian W -- Maricic, Tomislav -- Stenzel, Udo -- Kircher, Martin -- Patterson, Nick -- Li, Heng -- Zhai, Weiwei -- Fritz, Markus Hsi-Yang -- Hansen, Nancy F -- Durand, Eric Y -- Malaspinas, Anna-Sapfo -- Jensen, Jeffrey D -- Marques-Bonet, Tomas -- Alkan, Can -- Prufer, Kay -- Meyer, Matthias -- Burbano, Hernan A -- Good, Jeffrey M -- Schultz, Rigo -- Aximu-Petri, Ayinuer -- Butthof, Anne -- Hober, Barbara -- Hoffner, Barbara -- Siegemund, Madlen -- Weihmann, Antje -- Nusbaum, Chad -- Lander, Eric S -- Russ, Carsten -- Novod, Nathaniel -- Affourtit, Jason -- Egholm, Michael -- Verna, Christine -- Rudan, Pavao -- Brajkovic, Dejana -- Kucan, Zeljko -- Gusic, Ivan -- Doronichev, Vladimir B -- Golovanova, Liubov V -- Lalueza-Fox, Carles -- de la Rasilla, Marco -- Fortea, Javier -- Rosas, Antonio -- Schmitz, Ralf W -- Johnson, Philip L F -- Eichler, Evan E -- Falush, Daniel -- Birney, Ewan -- Mullikin, James C -- Slatkin, Montgomery -- Nielsen, Rasmus -- Kelso, Janet -- Lachmann, Michael -- Reich, David -- Paabo, Svante -- GM40282/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- Intramural NIH HHS/ -- New York, N.Y. -- Science. 2010 May 7;328(5979):710-22. doi: 10.1126/science.1188021.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Evolutionary Genetics, Max-Planck Institute for Evolutionary Anthropology, D-04103 Leipzig, Germany. green@eva.mpg.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20448178" target="_blank"〉PubMed〈/a〉
    Keywords: African Continental Ancestry Group/genetics ; Animals ; Asian Continental Ancestry Group/genetics ; Base Sequence ; Bone and Bones ; DNA, Mitochondrial/genetics ; European Continental Ancestry Group/genetics ; Evolution, Molecular ; Extinction, Biological ; Female ; *Fossils ; Gene Dosage ; Gene Flow ; Genetic Variation ; *Genome ; *Genome, Human ; Haplotypes ; Hominidae/*genetics ; Humans ; Pan troglodytes/genetics ; Polymorphism, Single Nucleotide ; Selection, Genetic ; Sequence Alignment ; *Sequence Analysis, DNA ; Time
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  • 10
    Publication Date: 2013-05-25
    Description: Key insights into the behavior of materials can be gained by observing their structure as they undergo lattice distortion. Laser pulses on the femtosecond time scale can be used to induce disorder in a "pump-probe" experiment with the ensuing transients being probed stroboscopically with femtosecond pulses of visible light, x-rays, or electrons. Here we report three-dimensional imaging of the generation and subsequent evolution of coherent acoustic phonons on the picosecond time scale within a single gold nanocrystal by means of an x-ray free-electron laser, providing insights into the physics of this phenomenon. Our results allow comparison and confirmation of predictive models based on continuum elasticity theory and molecular dynamics simulations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Clark, J N -- Beitra, L -- Xiong, G -- Higginbotham, A -- Fritz, D M -- Lemke, H T -- Zhu, D -- Chollet, M -- Williams, G J -- Messerschmidt, M -- Abbey, B -- Harder, R J -- Korsunsky, A M -- Wark, J S -- Robinson, I K -- BB/H022597/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- New York, N.Y. -- Science. 2013 Jul 5;341(6141):56-9. doi: 10.1126/science.1236034. Epub 2013 May 23.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉London Centre for Nanotechnology, University College London, London, UK. jesse.clark@ucl.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23704372" target="_blank"〉PubMed〈/a〉
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