Publication Date:
2004-10-09
Description:
We report that Id knockout mouse embryos display multiple cardiac defects, but mid-gestation lethality is rescued by the injection of 15 wild-type embryonic stem (ES) cells into mutant blastocysts. Myocardial markers altered in Id mutant cells are restored to normal throughout the chimeric myocardium. Intraperitoneal injection of ES cells into female mice before conception also partially rescues the cardiac phenotype with no incorporation of ES cells. Insulin-like growth factor 1, a long-range secreted factor, in combination with WNT5a, a locally secreted factor, likely account for complete reversion of the cardiac phenotype. Thus, ES cells have the potential to reverse congenital defects through Id-dependent local and long-range effects in a mammalian embryo.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1351017/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1351017/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fraidenraich, Diego -- Stillwell, Elizabeth -- Romero, Elizabeth -- Wilkes, David -- Manova, Katia -- Basson, Craig T -- Benezra, Robert -- K01 HL076568/HL/NHLBI NIH HHS/ -- KO1HL076568/HL/NHLBI NIH HHS/ -- R01 CA107429/CA/NCI NIH HHS/ -- R01CA107429/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2004 Oct 8;306(5694):247-52.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cancer Biology and Genetics Program, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15472070" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blastocyst
;
Cell Division
;
Cells, Cultured
;
DNA-Binding Proteins/genetics
;
Embryo Loss
;
Embryo, Mammalian/*cytology
;
Female
;
Gene Expression Profiling
;
Gene Expression Regulation, Developmental
;
Heart/*embryology
;
Heart Defects, Congenital/embryology/*therapy
;
Inhibitor of Differentiation Protein 1
;
Inhibitor of Differentiation Protein 2
;
Insulin-Like Growth Factor I/genetics/physiology
;
Maternal-Fetal Exchange
;
Mice
;
Mice, Knockout
;
Myocardium/cytology/metabolism
;
Myocytes, Cardiac/cytology
;
Oligonucleotide Array Sequence Analysis
;
Pericardium/embryology/metabolism
;
Pregnancy
;
Proto-Oncogene Proteins/genetics/physiology
;
Repressor Proteins/genetics
;
*Stem Cell Transplantation
;
Stem Cells/*physiology
;
Transcription Factors/genetics
;
Wnt Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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