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  • 1
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    Mapping of catalytic residues in the RNA polymerase active center (1996)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1996-07-05
    Description: When the Mg2+ ion in the catalytic center of Escherichia coli RNA polymerase (RNAP) is replaced with Fe2+, hydroxyl radicals are generated. In the promoter complex, such radicals cleave template DNA near the transcription start site, whereas the beta' subunit is cleaved at a conserved motif NADFDGD (Asn-Ala-Asp-Phe-Asp-Gly-Asp). Substitution of the three aspartate residues with alanine creates a dominant lethal mutation. The mutant RNAP is catalytically inactive but can bind promoters and form an open complex. The mutant fails to support Fe2+-induced cleavage of DNA or protein. Thus, the NAD-FDGD motif is involved in chelation of the active center Mg2+.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zaychikov, E -- Martin, E -- Denissova, L -- Kozlov, M -- Markovtsov, V -- Kashlev, M -- Heumann, H -- Nikiforov, V -- Goldfarb, A -- Mustaev, A -- New York, N.Y. -- Science. 1996 Jul 5;273(5271):107-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Limnological Institute, Russian Academy of Sciences, Irkutsk, Russia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8658176" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Aspartic Acid/metabolism ; Binding Sites ; DNA/metabolism ; DNA-Directed RNA Polymerases/*chemistry/genetics/*metabolism ; Dithiothreitol/pharmacology ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli/*enzymology ; Ferrous Compounds/metabolism ; Hydroxyl Radical ; Magnesium/metabolism ; Molecular Sequence Data ; Mutagenesis ; Promoter Regions, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    Differential cross sections for state specific reactive scattering of Na–SF6→NaF–SF5 (1996)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 104 (1996), S. 3620-3628 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Double-differential cross sections for the interaction of Na(3 2S) and Na(3 2P) with SF6 have been measured in crossed beam experiments for center of mass collision energies between 0.25 and 1.75 eV. In comparison with recently reported experiments the reaction with vibrationally excited SF6 is found to be more effective than the one with electronically excited Na. Results from an ab initio CASSCF calculation with Na in the ground state and the 3P state are presented. The experimental findings and the results from the calculation lead us to two different models for the reaction in the ground state and the excited state: While the well known harpooning model is verified for the ground state the reactive collisions with excited Na are mediated by nonadiabatic (nonreactive) transitions to the ground state surface. For these transitions the vibrational motion of SF6 is much more efficient than the relative motion in the collision. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.471064
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  • 3
    Electronic Resource
    Electronic Resource
    540 mW of blue output power at 425 nm generated by intracavity frequency doubling an upconversion-pumped Er3+:YLiF4 laser (1998)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 73 (1998), S. 139-141 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report efficient room-temperature continuous-wave intracavity frequency doubling of an upconversion-pumped Er(1%):YLiF4 laser at 850 nm. A titanium–sapphire laser was used for excitation of the 4S3/2→4I13/2 transition in erbium. The maximum laser output power at 850 nm was 1200 mW. Intracavity frequency doubling the fundamental wave utilizing lithium triborate as nonlinear crystal yielded a maximum second-harmonic output power of 540 mW at 425 nm. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.121735
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