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  • 1
    Publication Date: 2016-08-05
    Description: Forsterite (Mg 2 SiO 4 ) is one of the major planetary materials, and its behavior under extreme conditions is important to understand the interior structure of large planets, such as super-Earths, and large-scale planetary impact events. Previous shock compression measurements of forsterite indicate that it may melt below 200 GPa, but these measurements did not go beyond 200 GPa. We report the shock response of forsterite above ~250 GPa, obtained using the laser shock wave technique. We simultaneously measured the Hugoniot and temperature of shocked forsterite and interpreted the results to suggest the following: (i) incongruent crystallization of MgO at 271 to 285 GPa, (ii) phase transition of MgO at 285 to 344 GPa, and (iii) remelting above ~470 to 500 GPa. These exothermic and endothermic reactions are seen to occur under extreme conditions of pressure and temperature. They indicate complex structural and chemical changes in the system MgO-SiO 2 at extreme pressures and temperatures and will affect the way we understand the interior processes of large rocky planets as well as material transformation by impacts in the formation of planetary systems.
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 2
    Publication Date: 2016-03-27
    Description: Thg1-like protein (TLP) catalyzes the addition of a nucleotide to the 5'-end of truncated transfer RNA (tRNA) species in a Watson-Crick template–dependent manner. The reaction proceeds in two steps: the activation of the 5'-end by adenosine 5'-triphosphate (ATP)/guanosine 5'-triphosphate (GTP), followed by nucleotide addition. Structural analyses of the TLP and its reaction intermediates have revealed the atomic detail of the template-dependent elongation reaction in the 3'-5' direction. The enzyme creates two substrate binding sites for the first- and second-step reactions in the vicinity of one reaction center consisting of two Mg 2+ ions, and the two reactions are executed at the same reaction center in a stepwise fashion. When the incoming nucleotide is bound to the second binding site with Watson-Crick hydrogen bonds, the 3'-OH of the incoming nucleotide and the 5'-triphosphate of the tRNA are moved to the reaction center where the first reaction has occurred. That the 3'-5' elongation enzyme performs this elaborate two-step reaction in one catalytic center suggests that these two reactions have been inseparable throughout the process of protein evolution. Although TLP and Thg1 have similar tetrameric organization, the tRNA binding mode of TLP is different from that of Thg1, a tRNA His -specific G –1 addition enzyme. Each tRNA His binds to three of the four Thg1 tetramer subunits, whereas in TLP, tRNA only binds to a dimer interface and the elongation reaction is terminated by measuring the accepter stem length through the flexible β-hairpin. Furthermore, mutational analyses show that tRNA His is bound to TLP in a similar manner as Thg1, thus indicating that TLP has a dual binding mode.
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 3
    Publication Date: 2019
    Description: 〈p〉V〈sub〉1〈/sub〉-ATPase is an ATP-driven rotary motor that is composed of a ring-shaped A〈sub〉3〈/sub〉B〈sub〉3〈/sub〉 complex and a central DF shaft. The nucleotide-free A〈sub〉3〈/sub〉B〈sub〉3〈/sub〉 complex of 〈i〉Enterococcus hirae〈/i〉, composed of three identical A〈sub〉1〈/sub〉B〈sub〉1〈/sub〉 heterodimers, showed a unique asymmetrical structure, probably due to the strong binding of the N-terminal barrel domain, which forms a crown structure. Here, we mutated the barrel region to weaken the crown, and performed structural analyses using high-speed atomic force microscopy and x-ray crystallography of the mutant A〈sub〉3〈/sub〉B〈sub〉3〈/sub〉. The nucleotide-free mutant A〈sub〉3〈/sub〉B〈sub〉3〈/sub〉 complex had a more symmetrical open structure than the wild type. Binding of nucleotides produced a closely packed spiral-like structure with a disrupted crown. These findings suggest that wild-type A〈sub〉3〈/sub〉B〈sub〉3〈/sub〉 forms a metastable (stressed) asymmetric structure composed of unstable A〈sub〉1〈/sub〉B〈sub〉1〈/sub〉 conformers due to the strong constraint of the crown. The results further the understanding of the principle of the cooperative transition mechanism of rotary motors.〈/p〉
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 4
    Publication Date: 2018-06-07
    Description: The nucleolus is a dynamic nuclear body that has been demonstrated to disassemble at the onset of mitosis; the relationship between cell cycle progression and nucleolar integrity, however, remains poorly understood. We studied the role of nucleolar proteins in mitosis by performing a global analysis using small interfering RNAs specific to nucleolar proteins; we focused on nucleolar protein 11 (NOL11), with currently unknown mitotic functions. Depletion of NOL11 delayed entry into the mitotic phase owing to increased inhibitory phosphorylation of cyclin-dependent kinase 1 (Cdk1) and aberrant accumulation of Wee1, a kinase that phosphorylates and inhibits Cdk1. In addition to effects on overall mitotic phenotypes, NOL11 depletion reduced ribosomal RNA (rRNA) levels and caused nucleolar disruption during interphase. Notably, mitotic phenotypes found in NOL11-depleted cells were recapitulated when nucleolar disruption was induced by depletion of rRNA transcription factors or treatment with actinomycin D. Furthermore, delayed entry into the mitotic phase, caused by the depletion of pre-rRNA transcription factors, was attributable to nucleolar disruption rather than to G 2 /M checkpoint activation or reduced protein synthesis. Our findings therefore suggest that maintenance of nucleolar integrity during interphase is essential for proper cell cycle progression to mitosis via the regulation of Wee1 and Cdk1.
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 5
    Publication Date: 2015-06-06
    Description: The manipulation of domains by external fields in ferroic materials is of major interest for applications. In multiferroics with strongly coupled magnetic and electric order, however, the magnetoelectric coupling on the level of the domains is largely unexplored. We investigated the field-induced domain dynamics of TbMnO3 in the multiferroic ground state and across a first-order spin-flop transition. In spite of the discontinuous nature of this transition, the reorientation of the order parameters is deterministic and preserves the multiferroic domain pattern. Landau-Lifshitz-Gilbert simulations reveal that this behavior is intrinsic. Such magnetoelectric correlations in spin-driven ferroelectrics may lead to domain wall-based nanoelectronics devices.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Matsubara, Masakazu -- Manz, Sebastian -- Mochizuki, Masahito -- Kubacka, Teresa -- Iyama, Ayato -- Aliouane, Nadir -- Kimura, Tsuyoshi -- Johnson, Steven L -- Meier, Dennis -- Fiebig, Manfred -- New York, N.Y. -- Science. 2015 Jun 5;348(6239):1112-5. doi: 10.1126/science.1260561.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland. Department of Physics, Tohoku University, Sendai 980-8578, Japan. m-matsubara@m.tohoku.ac.jp sebastian.manz@mat.ethz.ch. ; Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland. m-matsubara@m.tohoku.ac.jp sebastian.manz@mat.ethz.ch. ; Department of Physics and Mathematics, Aoyama Gakuin University, Sagamihara, Kanagawa 229-8558, Japan. PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan. ; Department of Physics, ETH Zurich, Auguste-Piccard-Hof 1, 8093 Zurich, Switzerland. ; Division of Materials Physics, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan. ; Laboratory for Neutron Scattering and Imaging, Paul Scherrer Institute, 5232 Villigen, Switzerland. ; Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26045431" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
    Publication Date: 2016-03-19
    Description: The crystal structure of ionic nanocrystals (NCs) is usually controlled through reaction temperature, according to their phase diagram. We show that when ionic NCs with different shapes, but identical crystal structures, were subjected to anion exchange reactions under ambient conditions, pseudomorphic products with different crystal systems were obtained. The shape-dependent anionic framework (surface anion sublattice and stacking pattern) of Cu2O NCs determined the crystal system of anion-exchanged products of CuxS nanocages. This method enabled us to convert a body-centered cubic lattice into either a face-centered cubic or a hexagonally close-packed lattice to form crystallographically unusual, multiply twinned structures. Subsequent cation exchange reactions produced CdS nanocages while preserving the multiply-twinned structures. A high-temperature stable phase such as wurtzite ZnS was also obtained with this method at ambient conditions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wu, Hsin-Lun -- Sato, Ryota -- Yamaguchi, Atsushi -- Kimura, Masato -- Haruta, Mitsutaka -- Kurata, Hiroki -- Teranishi, Toshiharu -- New York, N.Y. -- Science. 2016 Mar 18;351(6279):1306-10. doi: 10.1126/science.aad5520.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. ; Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. teranisi@scl.kyoto-u.ac.jp.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26989249" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
    Publication Date: 2016-04-16
    Description: Interstellar dust (ISD) is the condensed phase of the interstellar medium. In situ data from the Cosmic Dust Analyzer on board the Cassini spacecraft reveal that the Saturnian system is passed by ISD grains from our immediate interstellar neighborhood, the local interstellar cloud. We determine the mass distribution of 36 interstellar grains, their elemental composition, and a lower limit for the ISD flux at Saturn. Mass spectra and grain dynamics suggest the presence of magnesium-rich grains of silicate and oxide composition, partly with iron inclusions. Major rock-forming elements (magnesium, silicon, iron, and calcium) are present in cosmic abundances, with only small grain-to-grain variations, but sulfur and carbon are depleted. The ISD grains in the solar neighborhood appear to be homogenized, likely by repeated processing in the interstellar medium.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Altobelli, N -- Postberg, F -- Fiege, K -- Trieloff, M -- Kimura, H -- Sterken, V J -- Hsu, H-W -- Hillier, J -- Khawaja, N -- Moragas-Klostermeyer, G -- Blum, J -- Burton, M -- Srama, R -- Kempf, S -- Gruen, E -- New York, N.Y. -- Science. 2016 Apr 15;352(6283):312-8. doi: 10.1126/science.aac6397.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Space Agency, European Space Astronomy Centre, Madrid, Spain. nicolas.altobelli@sciops.esa.int. ; Institut fur Geowissenschaften, University of Heidelberg, Heidelberg, Germany. Institut fur Raumfahrtsysteme, University of Stuttgart, Stuttgart, Germany. ; Institut fur Geowissenschaften, University of Heidelberg, Heidelberg, Germany. Georgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta, GA, USA. ; Institut fur Geowissenschaften, University of Heidelberg, Heidelberg, Germany. Klaus-Tschira-Labor fur Kosmochemie, University of Heidelberg, Heidelberg, Germany. ; Kobe University, Kobe, Hyogo, Japan. ; International Space Sciences Institute, Bern, Switzerland. ; University of Boulder, Boulder, CO, USA. ; University of Kent, Kent, UK. ; Institut fur Raumfahrtsysteme, University of Stuttgart, Stuttgart, Germany. ; Technische Universitat Braunschweig, Institut fur Geophysik und Extraterrestrische Physik, Braunschweig, Germany. ; Jet Propulsion Laboratory, Pasadena, CA, USA. ; Institut fur Geowissenschaften, University of Heidelberg, Heidelberg, Germany. Institut fur Raumfahrtsysteme, University of Stuttgart, Stuttgart, Germany. University of Boulder, Boulder, CO, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/27081064" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
    Publication Date: 2016-03-12
    Description: Poly(ethylene terephthalate) (PET) is used extensively worldwide in plastic products, and its accumulation in the environment has become a global concern. Because the ability to enzymatically degrade PET has been thought to be limited to a few fungal species, biodegradation is not yet a viable remediation or recycling strategy. By screening natural microbial communities exposed to PET in the environment, we isolated a novel bacterium, Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. When grown on PET, this strain produces two enzymes capable of hydrolyzing PET and the reaction intermediate, mono(2-hydroxyethyl) terephthalic acid. Both enzymes are required to enzymatically convert PET efficiently into its two environmentally benign monomers, terephthalic acid and ethylene glycol.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoshida, Shosuke -- Hiraga, Kazumi -- Takehana, Toshihiko -- Taniguchi, Ikuo -- Yamaji, Hironao -- Maeda, Yasuhito -- Toyohara, Kiyotsuna -- Miyamoto, Kenji -- Kimura, Yoshiharu -- Oda, Kohei -- New York, N.Y. -- Science. 2016 Mar 11;351(6278):1196-9. doi: 10.1126/science.aad6359.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan. ; Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ; Life Science Materials Laboratory, ADEKA, 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan. ; Department of Polymer Science, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ; Ecology-Related Material Group Innovation Research Institute, Teijin, Hinode-cho 2-1, Iwakuni, Yamaguchi 740-8511, Japan. ; Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26965627" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Betaproteobacteria/*enzymology ; Environmental Restoration and Remediation ; Enzymes/classification/genetics/metabolism ; Hydrolysis ; Microbial Consortia ; Molecular Sequence Data ; Phthalic Acids/metabolism ; Phylogeny ; Plastics/*metabolism ; Polyethylene Terephthalates/*metabolism ; Recycling
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 2017-06-16
    Description: The discovery of slow earthquakes has revolutionized the field of earthquake seismology. Defining the locations of these events and the conditions that favor their occurrence provides important insights into the slip behavior of tectonic faults. We report on a family of recurring slow-slip events (SSEs) on the plate interface immediately seaward of repeated historical moment magnitude ( M w ) 8 earthquake rupture areas offshore of Japan. The SSEs continue for days to several weeks, include both spontaneous and triggered slip, recur every 8 to 15 months, and are accompanied by swarms of low-frequency tremors. We can explain the SSEs with 1 to 4 centimeters of slip along the megathrust, centered 25 to 35 kilometers (km) from the trench (4 to 10 km depth). The SSEs accommodate 30 to 55% of the plate motion, indicating frequent release of accumulated strain near the trench.
    Keywords: Geochemistry, Geophysics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
    Publication Date: 2017-10-01
    Description: Interstellar ice is believed to be a cradle of complex organic compounds, commonly found within icy comets and interstellar clouds, in association with ultraviolet (UV) irradiation and subsequent warming. We found that UV-irradiated amorphous ices composed of H 2 O, CH 3 OH, and NH 3 and of pure H 2 O behave like liquids over the temperature ranges of 65 to 150 kelvin and 50 to 140 kelvin, respectively. This low-viscosity liquid-like ice may enhance the formation of organic compounds including prebiotic molecules and the accretion of icy dust to form icy planetesimals under certain interstellar conditions.
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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