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  • 1
    Publication Date: 2012-06-15
    Description: Background: Single-stranded DNA binding proteins (SSB) are essential for DNA replication, repair, and recombination in all organisms. SSB works in concert with a variety of DNA metabolizing enzymes such as DNA polymerase. Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations [less than or equal to]100 mug/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high affinity comparable to other prokaryotic SSBs. Thermodynamic analysis indicated both hydrophobic and ionic contributions to ssDNA binding. FRET analysis of oligo(dT)70 binding suggested that SSBBA forms a tetrameric assembly upon ssDNA binding. This report provides evidence of a bacterial SSB that utilizes a novel mechanism for DNA binding through the formation of a transient tetrameric structure. Conclusions: Unlike other prokaryotic SSB proteins, SSBBA from Bacillus anthracis appeared to be monomeric at concentrations [less than or equal to]100 mug/ml as determined by SE-HPLC. SSBBA retained its ability to bind ssDNA with very high affinity, comparable to SSB proteins which are tetrameric. In the presence of a long ssDNA template, SSBBA appears to form a transient tetrameric structure. Its unique structure appears to be due to the cumulative effect of multiple key amino acid changes in its sequence during evolution, leading to perturbation of stable dimer and tetramer formation. The structural features of SSBBA could promote facile assembly and disassembly of the protein-DNA complex required in processes such as DNA replication.
    Electronic ISSN: 1471-2091
    Topics: Chemistry and Pharmacology
    Published by BioMed Central
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 127 (1931), S. 166-166 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A MISTAKE in nomenclature has, through our inadvertence, crept into our communication on the “Synthesis of Munjisthin”, published in NATURE of Nov. 15, 1930, page 761, which we hasten to rectify. In line 6, par. 2, instead of " 2 - chloro - 3 methyl -4 methoxy ", read " 1 - chloro - 2 ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 213 (1967), S. 917-918 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Eleven common steroids of both plant and animal origin were selected at random (ranging from C19 to C30). These were as follows: cholesterol (C27); (3-sitosterol (C29); cholesteryl acetate (C29); lanosterol (C30); A-5-3-acetoxy-bisnorcholenic acid (C24); digitalin (C23); glycocholic acid (C26); ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 207 (1965), S. 215-216 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The methods used to study the absorption of 14C-propazine by detached tea leaves were slightly modified from those described by Biswas1, and Biswas and Rogers3, to study the absorption of s-triazine herbicides and gibberellic acid respectively. One variety of tea, 'Nyasaland', was grown at 65 -75 ...
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 209 (1966), S. 218-219 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The plankton and the various physico-chemical factors of the water are being investigated by members of the Volta Basin Research Project of the University of Ghana1. An intensive investigation has been undertaken of samples collected at Ajena, 2 miles north of the dam site at Akosombo. At first, ...
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 311 (1984), S. 477-480 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We use 5'-fluorosulphonylbenzoyl[14C]adenosine(5'-Jp-FSO2bz[14C]A) to screen for kinase site-containing fragments in tryptic digests of the solubilized purified receptor10'11. This ATP analogue irreversibly modifies ATP-binding sites12'14, and inhibits phosphorylation of both the solubilized ...
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  • 7
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Previous measurements of total glucose transporters in adipocytes from streptozotocin-induced diabetic and fasted rats have shown that the microsomal pool of intracellular glucose transporters is reduced concomitantly with the development of insulin resistance1"3. Insulin-dependent movement of the ...
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 321 (1986), S. 394-401 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Chaos is an inherent feature of many nonlinear systems. In particular, the transition from a steady to chaotic state occurs independently of the physical properties of the system. Such behaviour occurs in optics, both in lasers and in nonlinear optical devices. Such devices, which are fundamentally ...
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  • 9
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 187 (1960), S. 620-621 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1. RESPIRATION-RATES OF RICE SEEDS WITHOUT HUSK Year of //l./Oo/hr./mgm. //l./CO?/hr./mgm. collection dry weight dry weight 1953 0-078 0-054 1957 0-078 0-080 1958 0-142 0-113 NO 4737 August 13, 1960 NATURE 621 Table 2. ROOT AND SHOOT GROWTH (CM.) IN SOLUTIONS LEACHED FROM SEEDS OF RICE ...
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1219-1220 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Seven-day old cultures of Chlorella pyrenoidosa and Sce ±edesmus bliquus were centrifuged down at 12 C. in an MSE centrifuge and resuspended in 10 ml. phosphate buffer of pH. 6-8. The cells were then incubated with different concentrations of thioctic acid and 30 JJLC. of sodium ...
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