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  • 1955-1959  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 46 (1956), S. 423-436 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. When one end of an internodial cell ofNitella is brought in contact with water, and the other end with the solution of sucrose, transcellular osmosis takes place from water side to the sucrose side. 2. Accompanying transcellular osmosis, the cell sap on the water side is diluted and that on the sucrose side is concentrated. The magnitude of the polar change in sap concentration is dependent on the difference in osmotic pressure between the two external solutions. 3. By tying off an internodial cell ofNitella with strips of silk thread after inducing transcellular osmosis, it is possible to produce cells having arbitrary osmotic pressures within the range of as high as 3 times and as low as 1/4 the normal level. 4. The cells thus produced, which are in fact fragments of the mother internodial cell, can survive indefinitely. 5. The cell, whose osmotic pressure is abnormally high or low, has a marked tendency to be restored to its normal level. 6. The tendency to be restored to the normal osmotic pressure is maintained, if not fully, even when the import or export of solutes to/from the cell is prevented. 7. Turgor pressure of the cell plays no essential role in the osmoregulation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 50 (1958), S. 144-148 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The present work is a first attempt at calculating the absolute amount of the motive force responsible for the rotational protoplasmic streaming. The calculation was made on the basis of the conclusion we arrived at previously through the analysis of intracellular velocity distribution, namely, that the active driving mechanism responsible for the rotational streaming is located at the interface between the cortical gel and the outer edge of the endoplasmic layer. The motive force, which is the shifting force generated at this interface, was determined in the internodal cell ofNitella flexilis to be within the range of 1–2 dynes/cm2 at room temperature.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 48 (1957), S. 94-112 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The motive force responsible for the protoplasmic streaming in the myxomycete plasmodium,Physarum polycephalum, was measured by means of the double-chamber method under the influence of various chemical agents. 2. The motive force rather increases than decreases under anaerobic state as well as under the effect of KCN. The process is reversible. 3. Monoiodoacetic acid and sodium fluoride, both fermentation poisons, decrease the strength of the force back of the protoplasmic streaming. 4. 2,4-dinitrophenol inhibits the motive force production both under aerobic and anaerobic conditions. 5. ATP admitted from without markedly increases the motive force of the protoplasmic streaming. The effect is manifested several minutes after the reagent is applied and continues several ten minutes after the reagent is removed. 6. As the fermentation poisons depresses the generation of the motive force while conditions inhibiting respiration do not depress it, it was concluded that the direct energy source of the protoplasmic streaming is ATP synthesized by fermentation process.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 46 (1956), S. 394-422 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 49 (1958), S. 1-4 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary It was shown that the velocity distribution of the intracapillary streaming of protoplasm in a plasmodium ofPhysarum polycephalum is the same no matter whether the flow is spontaneous or whether it is induced artificially by external local air pressure applied to the plasmodium. Thus we conclude that the protoplasmic flow in the plasmodium is caused by local difference in endoplasm pressure. The view that the seat of the motive force responsible for the flow is located in the streaming protoplasm itself is untenable for this type of streaming.
    Type of Medium: Electronic Resource
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