ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A Tris buffer system (starting buffer, 0.04M Tris phosphate, pH 9.0; limiting buffer, 0.5M Tris H2PO4, pH 3.6) and a concave gradient elution procedure were developed for the fractionation of beef sarcoplasmic proteins by diethylaminoethyl cellulose ion-exchange chromatography. Sarcoplasmic proteins extracted 2 hr post-mortem were separated chromatographically into 16–18 distinct fractions. Duplication of the results was excellent. Some alterations occurring in these proteins during 10 days' post-mortem aging were detected by this technique. Such changes observed were the disappearance of one fraction and appearance of new components, while other fractions diminished.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1965.tb01816.x
Permalink
