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  • 1965-1969  (8)
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  • 1
    Publication Date: 1967-01-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 2
    Publication Date: 1969-01-01
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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  • 3
    Publication Date: 1968-01-01
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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  • 4
    Publication Date: 1968-01-01
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 14 (1968), S. 72-80 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Zur Klärung der selektiven Darstellbarkeit von Noradrenalin nach Fixierung mit Glutardialdehyd wurden Modellversuche durchgeführt. Die Beständigkeit der geprüften Reaktionsprodukte auch im sauren Milieu spricht gegen das Vorliegen einer Schiff schen Base, wohl aber zusammen mit dem negativen Ausfall der Ninhydrinreaktion für einen — bei Dicarbonylverbindungen ohnehin typischen — Ringschluß des Glutardialdehyd an der primären Aminogruppe von Noradrenalin oder Hydroxytyramin. Die phenolischen OH-Gruppen nehmen an der Reaktion nicht direkt teil. Das Amin-Dialdehyd-Umsetzungsprodukt ist in wäßrigen Lösungen schwerer löslich als das Amin selbst und verbleibt im Gewebe. Da allerdings auch nach Alkoholextraktion von Nebennieren noch Noradrenalin nachweisbar ist, muß zumindest eine teilweise Verankerung an Gewebsbestandteile vorliegen. Durch verschiedene Puffer mit physiologischem pH werden die Vesikelmembranen der Catecholamingranula zunächst nicht geschädigt, sondern offenbar erst unter Aldehydeinfluß für Amine passierbar. Tritt eine Reaktion mit Dialdehyd ein, so verbleibt die resultierende Verbindung im Gewebe, sonst kommt es zur Ausschwemmung noch während der Einwirkung der wäßrigen Aldehydlösung.
    Notes: Summary Model experiments were carried out to understand the selective demonstrability of noradrenaline in consequence of fixation with glutaraldehyde. The obvious stability of the reaction products tested even at low pH is in contradiction to the assumption that Schiff bases are formed. Rather together with a negative ninhydrine reaction this strongly suggests a cycle reaction of glutaraldehyde at the primary amino group of noradrenaline or hydroxytyramine. This cycle reaction is a type, typical of dicarbonyl compounds anyhow. The phenolic OH-groups of the amines are not involved directly. As the compound formed by primary amine and dialdehyde is less soluble in aquous media than the amines, it will remain in the tissue. Even after extracting suprarenal glands with aethanol, however, there still is a positive chromatereaction with noradrenaline. Therefore there must be a linkage of the noradrenaline at least in parts to tissue structures. Various buffer solutions at physiologic pH do not alter the membrane of amine granules. Rather destruction of membranes is obviously caused by aldehydes, thereby increasing their permeability for amines. If there is a reaction of amine with the dialdehyde the compound thus formed will remain in the tissue, if not the amine will be washed out from it, even before fixation with the aquous solution of an aldehyde has come to an end.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 17 (1969), S. 312-318 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Der Einfluß einiger Aldehyde und Carnoyscher Flüssigkeit auf das Eindringvermögen von Lösungen verschiedener Eisenverbindungen wurde untersucht. Bei allen Fixationsmedien und Eisenlösungen reagieren nur die Randpartien der Gewebeproben. Offensichtlich werden Gewebekomponenten durch Reaktion mit dem Eisen für dieses impermeabel.
    Notes: Summary The effect of some aldehydes and of Carnoy's fluid on the penetration of solutions of different iron compounds has been investigated. Independent of the fixation medium and the iron compound used only the peripheral zone of tissue samples do react. This seems to be due to the reaction of iron with tissue components making them impermeable to iron.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 14 (1968), S. 297-299 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cr could be demonstrated in reaction products of adrenaline and noradrenaline with K2Cr2O7 by spot test analysis. The strong contrast of amine granules in the electron microscope partly may be due to the presence of Cr within them. When KJO3 is used for oxydizing amines, only a slight contrast of granules can be observed in the electron microscope.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 83 (1967), S. 117-132 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Adrenalin-Granula lassen sich elektronenmikroskopisch im Mark von Goldhamsterfeten bereits 2 Tage vor der Geburt und damit 3 Tage früher als lichtmikroskopisch-histochemisch erkennen. Sie sind zu Beginn 0,1 μm groß und nach Kalium-Bichromatvorbehandlung des Gewebes von den 15 Tage nach der Geburt auftretenden Noradrenalin-Granula gut zu unterscheiden. Im weiteren Verlauf der postnatalen Entwicklung nehmen die beiden Granulatypen unterschiedlich an Größe zu. Der beim erwachsenen Goldhamster gefundene mittlere Durchmesser der Adrenalin-Granula beträgt 0,16±0,05 μm. Elektronenmikroskopisch-histochemisch können Adrenalin- und Noradrenalin-Granula auch dann eindeutig unterschieden werden, wenn sich ihre mittleren Durchmesser während eines begrenzten Entwicklungsabschnittes nicht signifikant unterscheiden. Die durch umgekehrte Micropinocytose aus dem Cytoplasma der Markzellen ausgeschleusten Granula konnten im Intercellularspalt, im interstitiellen Bindegewebe, in den Lumina der Markfollikel und Sinus und an Erythrozyten beobachtet werden.
    Notes: Summary Adrenaline granules can be seen in the electron microscope 2 days before birth in the adrenal medulla of the fetal golden hamster. This is 3 days earlier than by using histochemical methods in the light microscope. In the first time they are approximately 0.1 μm in diameter and can be well differentiated from noradrenaline granules after treatment of tissue with potassium-bichromate. In postnatal development the mean diameters of both adrenaline and noradrenaline granules increase in a different degree. So the mean diameter of adrenaline granules in adult animals is 0,16±0,05 μm whereas the one of noradrenaline granules comes to 0,26±0,05 μm. Using a histochemical method adrenaline and noradrenaline granules can be well differentiated in the electron microscope regardless if during a period in ontogenesis their mean diameters do not differ significantly. Granules are extruded from the cytoplasma of medullary cells by reversed micropinocytosis. Either the material of granules or granules itself could be observed in intercellular space, at the interstitial connective tissue, at the lumina of medullar follicles and sinus, and adsorbed at erythrocytes.
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