ISSN:
1432-0827
Keywords:
Rat incisor
;
Enamel
;
Microdissection
;
Phosphate
;
32P incorporation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Abstract To determine the sites at which ions were incorporated into the forming enamel of the rat incisor, the incorporation of32P-labelled phosphate has been studiedin vivo at different time intervals after intraperitoneal injection with the32P andin vitro. The enamel from the lower incisor teeth was dissected into a series of about twenty pieces and the variation in specific activity (S.A.) from the root apex to the mature tissue determined. In general, S.A. was high in the early forming enamel falling towards the maturing tissue. Ten minutes after injection, this fall was interrupted by one or sometimes two areas of relatively high S.A. One occurred at the beginning of the maturation zone and persisted until the enamel was worn away by attrition at the incisal tip. This seemed to reflect the acquisition of phosphate by accretion during enamel maturation. The second S.A. peak, which occurred in the earlier forming enamel, tended to appear at short intervals after injection and was rarely observed when the period after injection was extended to 24h. A S.A. peak at the same site was produced in the forming enamel when incisors were incubatedin vitro in32P phosphate. This second peak therefore seemed to reflect some change in the tissue's physical or chemical properties rather than cellular activity. No S.A. peak was obtainedin vitro in the maturing tissue.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02059052
Permalink
