ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hit 1 - 1 | 1 hit

Sorting

Electronic Resource

A method of indirect mutant selection in Physarum polycephalum using the antibiotic netropsin (1974)

Gorman, Jessica A. ; Dove, William F.

Springer

Molecular genetics and genomics 133 (1974), S. 345-351

add to mindlist on the mindlist

Details

ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A method of indirect mutant selection, analogous to the bacterial penicillin technique, has been developed for Physarum polycephalum. The antibiotic netropsin was found to be lethal to growing but not to nongrowing (cycloheximide inhibited) myxamoebae. In reconstruction experiments, a 200 fold enrichment of cycloheximide sensitive over cycloheximide resistant cells was obtained. This technique was used to isolate temperature sensitive mutants. The mutant frequency among survivors was increased at least 40 fold by netropsin selection.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00332710

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

hit 1 - 1 | 1 hit