ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A new method for applying the electron microscope autoradiography to negatively stained specimens is presented. Free ribosomes and ribosome crystals, labeled with 3H-uridine, have been isolated from the rat liver and from the whole hypothermic chick embryo, respectively. The samples, fixed and negatively stained with uranyl acetate, have been treated for autoradiography with the Kodak NTE nuclear emulsion. This method allows to obtain autoradiograms characterized by a very high resolution power of well stained specimens. The perspectives of this new field of ultrastructural investigation are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00303666
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