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  • 1
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fish eggs and larvae can be separated from invertebrate zooplankton by isopycnic centrifugation in gradients of sucrose or silica. Preserved samples of invertebrate zooplankton, fish eggs, and fish larvae, representing a typical assortment of marine plankton, were layered over linear gradients of 25 to 60% w/w (weight/weight) sucrose or 0 to 15% w/w silica (as Ludox AM) in 100 c3 swinging buckets, and centrifuged for 1 h at 1000 rpm (revs per minute). In sucrose gradients, the invertebrate zooplankton were confined to the two ends of the gradient, while 85% of the fish eggs were recovered from an intermediate zone (27.5 to 55% w/w). In Ludox AM, the fish eggs banded in a narrow region between 2 and 3% w/w, while fish larvae banded at the bottom of the gradient between 10 and 14% w/w. Of the 6 dominant classes of zooplankton, only Salpa overlapped appreciably with the fish eggs and none overlapped with the fish larvae. Of the gradient materials tested, Ludox AM offers the most advantages; sucrose may also be useful for subfractionation. Gradients of sodium bromide and dextran have been found to be totally unsuitable.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 70 (1970), S. 247-260 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Acid phosphatase, generally thought of as a lysosomal enzyme and indeed widely employed as a lysosomal marker, has been found associated with the Golgi complex of all cell types from the crop, intestine and digestive gland ofArion ater. Reaction product was also detected within the multivesicular bodies and cytoplasm of columnar cells from the crop and the multivesicular bodies of mucous cells from the intestine. A vacuolar localization was obtained in the digestive cells of the intestine and digestive gland. Secretory protein granules in the calcium cells of the same gland and apical vacuoles in the so-called thin cells also showed a positive reaction.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 71 (1970), S. 409-417 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Golgi associated acid phosphatase has been demonstrated within muscle and nerve cells from the sub-epithelial layers of the crop, intestine, and digestive gland ofArion ater. Enzyme activity was detected in the saccules, vacuoles, and vesicles of the Golgi apparatus of both nerve ganglia and muscle cells. Other vacuolar sources of acid phosphatase could also be distinguished within the cytoplasm of these cells.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 73 (1971), S. 73-81 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Acid phosphatase activity has been found in association with the extensive brush border of the excretory cells and also within the lysosomal vacuoles of actively phagocytosing digestive cells. A release of enzyme into the cytoplasm occurs in some cells of the digestive epithelium. This appears to be linked with cellular lysis and may be a prelude to cell replacement.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 79 (1974), S. 19-29 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using a variety of substrates and lead citrate as a capture reagent, alkaline phosphatase has been demonstrated at the fine structural level in the collecting ducts of the protonephridial system ofPolycelis tenuis. The enzyme was found to be associated with a glycocalyx on the luminal surface of the duct and in the Golgi and vesicles in the cells lining the duct. The enzyme has also been demonstrated on the membranes surrounding nerve, muscle, and fixed parenchyma cells at the base of the pharynx. Other positive sites include the goblet or mucous cells and some of the intercellular spaces in the gastrodermis. The significance of these findings is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 28 (1972), S. 1082-1083 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Nachweis, dass corticale Läsionen eine Akkumulation von Noradrenalin an der Läsionsstelle hervorrufen.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 28 (1972), S. 497-498 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Grundwasserproben aus der Gort-Ebene (West-Irland) wurden auf Tritium-Gehalt analysiert und die Ergebnisse mit dem bereits bekannten Tritium-Einstrom dieser Gegend verglichen. Registrierungen des «World Precipitation Network» in Wien ergaben den Nachweis eines offenbar langsamen Durchflusses in das Grundwasser-Aquifer-System.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 7 (1972), S. 125-132 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cell-free system prepared from immature seed of Cucurbita pepo incorporates the label from mevalonate-2-14C into ent-kaur-16-en-19-oic acid (I), ent-7α-hydroxy-kaur-16-en-19-oic acid (II), and ent-gibberell-16-en-7-al-19-oic acid (III) (gibberellin A12-aldehyde). The products were identified by gas liquid chromatography and by combined gas chromatography-mass spectrometry of the methylated and trimethylsilylated fractions. The radioactivity of the compounds was established by recrystallisation to constant specific radioactivity. Gibberellin A12 (IV), also detected in the system after incubation by combined gas chromatographymass spectrometry may be an artefact, derived from gibberellin A12-aldehyde by a non-enzymatic conversion. With gibberellin A12-aldehyde, the cell-free biosynthesis of an ent-gibberellane has been achieved for the first time.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Planta 99 (1971), S. 120-132 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of IAA and three synthetic auxins on the upward movement of 32P-orthophosphate and 14C-sucrose has been investigated in decapitated stems of Phaseolus vulgaris L., Pisum sativum L., Coleus blumci L. and Helianthus annuus L. IAA greatly enhanced the accumulation of 32P-orthophosphate in Phaseolus vulgaris and Pisum sativum, whereas in C. blumei and H. annuus it did not. 2,4-D like IAA, caused an increase in 14C-sucrose and 32P-orthophosphate accumulation in Phaseolus vulgaris but, unlike IAA, caused no increase in Pisum sativum. The downward transport of 14C-IAA, 14C-NAA, 14C-2,4-D and 14C-2,4,5-T from the decapitated apex was also studied. Results are discussed in terms of current theories of hormone-directed transport.
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