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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural Study of Plasmodium knowlesi Antigen Used in Vaccination of Rhesus Monkeys (1976)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Material from various steps obtained in the French pressure cell technic of preparing antigen from Plasmodium knowlesi-infected red cells, was examined by electron microscopy. A positively charged colloidal iron solution was used to differentiate between membranes of host red cells and parasites. Red cell membranes take the stain, whereas parasite membranes do not. This antigen which has been used previously to protect monkeys against P. knowlesi appears to consist almost entirely of membrane-bounded vesicles. Some of these vesicles contain a fine granular material, whereas others appear empty. The antigen failed to stain with the positively charged iron solution, which suggests that it is free of contamination by host cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1976.tb03806.x
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  • 2
    Electronic Resource
    Electronic Resource
    Fine Structure of the Oocyst Walls of Isospora serini and Isospora canaria and Excystation of Isospora serini From the Canary, Serinus canarius L. (1975)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 22 (1975), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Oocysts of Isospora serini and Isospora canaria, from the canary Serinus canarius, were broken, added to a cell suspension, fixed in Karnovsky's fluid, and studied in the electron microscope. The oocyst wall of each species had an electronlucent inner layer, a more osmiophilic middle layer and an outer layer of electron-lucent (I. serini) or electron-dense material interspersed with some electron-lucent material (I. canaria). A few, relatively large lipid-like bodies were present in the outer or middle layer of the oocyst wall of I. canaria. As many as 9 membranes were present in the oocyst wall of I. canaria and 3 in that of I. serini. When exposed to a trypsin-sodium taurocholate fluid, sporozoites of I. serini excysted from 5-month-old sporocysts in vitro, but not from sporocysts stored for more than 6 months. No excystation occurred in 15-month-old I. canaria sporocysts. Similarities and differences in excystation between I. serini and other Isospora, Eimeria, and Sarcocystis species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1975.tb05212.x
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  • 3
    Electronic Resource
    Electronic Resource
    Hammondia pardalis sp. n. (Sarcocystidae) from the Ocelot, Felis pardalis, and Experimental Infection of Other Felines (1979)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 26 (1979), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Synopsis. Hammondia pardals sp. n. (Eimeriorina: Sarcocystidae) from Panama Canal Zone is described as an obligate heteroxenous coccidian, with felids as the final host and laboratory mice as the experimental intermediate host. Ovoid oocysts. measuring 40.8 (36–46) × 28.5 (25–35) m̈m. are shed unsporulated. Oocysts were infective only for the intermediate host. the laboratory mouse, Mus musculus, and the intracellular cysts were infective only for felids. Attempted passage of tissue cysts from mouse to mouse was unsuccessful.Mice fed 5 × 104 sporulated oocysts were found to harbor small intracellular cysts, 13–16 × 10–15 m̈m, in the mesenteric lymph nodes, lungs, and intestinal submucosa 15 days postinfection. The meronts in these early cysts were stubby and measured 3 × 6 m̈m. The prepatent period in the felids was 5 to 8 days and the patent period 5–13 days. Experimental infections of definitive hosts were successful with 6/6 domestic laboratory-reared kittens, Felis catus; 5/5 ocelots, F. pardalis; and 1/1 jaguarundi, F. yagouaroundi. None of the exposed raccoons, Procyon lotor, shed oocysts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1979.tb02728.x
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  • 4
    Electronic Resource
    Electronic Resource
    Eimeria crotalviridis sp. n. from Prairie Rattlesnakes, Crotalus viridis viridis, in New Mexico with Data on Excystation of Sporozoites and Ultrastructure of the Oocyst Wall (1977)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 24 (1977), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS Oocysts of Eimeria crotalviridis sp. n. are described from prairie rattlesnakes, Crotalus viridis viridis in New Mexico on the basis of light and electron microscopy and in vitro excystation of sporozoites. Sporulated oocysts of E. crotalviridis are elliptical, 26.4 × 22.3 (23–29 × 20–24) μm with ovoid sporocysts 11.7 × 8.1 (11–13 × 7–9) μm. A micropyle, micropyle cap and polar bodies are absent, but oocyst and sporocyst residua and Stieda and substieda bodies are present. Excysted sporozoites are 12.4 × 2.8 (11–13 × 2–3) μm and have 1 large posterior refractile body and a nucleus with a prominent nucleolus. Ultrastructurally, the oocyst wall has 2 layers, a thick, electron-dense, highly sculptured outer layer composed of a fine granular matrix and a thin, granular, osmiophilic inner layer, separated from the outer layer by at least one unit membrane. These layers are 441 (353–510) and 21.6 (19–29) nm thick, respectively. Within 15 min after exposure to a trypsin-sodium taurocholate fluid, sporozoites of E. crotalviridis excysted from 5-month-old sporocysts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1977.tb04749.x
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  • 5
    Electronic Resource
    Electronic Resource
    Fine Structure of the Oocyst Wall of Eimeria nieschulzi (1978)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 25 (1978), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Oocysts of Eimeria nieschulzi from the laboratory rat, Rattus, norvegicus, were studied by scanning and transmission electron microscopy. Oocysts had a rough outer wall with apparent random depressions. The oocyst wall is composed of 2 layers: an osmiophilic outer layer consisting of a rough external and smooth internal surface, and a relatively thick, electron-lucent inner layer. The outer layer is composed of a dense, coarsely granular matrix. The inner layer consists of homogeneous fine granular material interspersed with coarse osmiophilic granules and contains one closely applied membrane on the outermost surface. Several raised lenticular areas are seen on the coarse outer surface of the inner layer. These layers are 102 (75–128) and 176 (135–204) nm thick, respectively.The sporocyst wall is thin, consisting of 3 to 4 unit membranes, and measures 27 (18–34) nm thick.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1978.tb04165.x
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  • 6
    Electronic Resource
    Electronic Resource
    Fine-Structural Aspects of Microgametogenesis of Eimeria magna in Rabbits and in Kidney Cell Cultures (1976)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The fine-structural aspects of development of microgamonts of Eimeria magna were studied in kidney cell cultures and in experimentally infected rabbits. Spheroidal masses of gamont-like cytoplasm containing ribosomes, polyribosomes, and amylopectin granules were found within the parasitophorous vacuole; these bodies were apparently pinched off the surface of the gamont. Nucleoli were present in the early stages of nuclear division but disappeared as development proceeded. Spindles were eccentric and the nuclear membrane always remained intact in dividing nuclei. Nuclei eventually became elongate in shape, compact, and electron dense at the end oriented toward the periphery and lucent centrally. Usually, only the dense portion was incorporated into the gamete as the gamete developed by protruding from the gamont surface. Fullyformed microgametes were biflagellate and contained a nucleus, a mitochondrion, and 8-10 microtubules. Multiple-membrane complexes, which apparently originated from the nuclear envelope or endoplasmic reticulum, were found within the gamont residual body. Occasionally, 2 or 3 micro- and/or macrogamonts were seen within the same cell. In some cells one or 2 micro- or macrogamonts as well as several merozoites were present in the same parasitophorous vacuole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1976.tb05252.x
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  • 7
    Electronic Resource
    Electronic Resource
    Distributional limits of renormalized Feynman integrals with zero-mass denominators (1976)
    Springer
    Communications in mathematical physics 47 (1976), S. 43-51 
    Details
    ISSN: 1432-0916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Abstract It is shown that the ε→0 limits of renormalized Feynman integrals exist and define Lorentz invariant tempered distributions in the external momenta. The proof applies to the case where some or all particle masses vanish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01609352
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  • 8
    Electronic Resource
    Electronic Resource
    Existence of conserved currents in the perturbative sine-Gordon and massive thirring models (1978)
    Springer
    Communications in mathematical physics 63 (1978), S. 97-112 
    Details
    ISSN: 1432-0916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Abstract The existence of infinitely many conserved currents in the quantized sine-Gordon and massive Thirring models is proved in renormalized perturbation theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01220847
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  • 9
    Electronic Resource
    Electronic Resource
    Excystation of Isospora arctopitheci Rodhain, 1933 with notes on a similar process in Isospora bigemina (Stiles, 1891) Lühe, 1906 (1976)
    Springer
    Parasitology research 48 (1976), S. 191-197 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The in vitro excystation process of sporozoites of Isospora arctopitheci Rodhain, 1933 from the titi marmoset Saguinus geoffroyi and of Isospora bigemina (Stiles, 1891) Lühe, 1906 from the bobcat, Lynx rufus is presented. Sporocysts of both species lack a Stieda body and when exposed to a trypsin-sodium taurocholate (pH 7.4) excysting fluid the walls of both collapse in a similar fashion, along apparently predetermined lines. Similarities and differences in excystation between I. arctopitheci, I. bigemina, and other Isospora, Eimeria, and Sarcocystis species are summarized. Such studies show that 2 distinct patterns of sporozoite excystation have been described to date, and both appear to be related to the structure of the sporocyst.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380392
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  • 10
    Electronic Resource
    Electronic Resource
    Cultivation of the erythrocytic stages ofPlasmodium berghei in Leydig cell tumor cultures (1976)
    Springer
    Parasitology research 50 (1976), S. 237-244 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Twelve different established cell-lines were used in attempts to cultivate the erythrocytic stages ofPlasmodium berghei, P. vinckei vinckei, P. coatneyi orP. knowlesi. Intracellular parasites were seen in only mouse Leydig cell testicular tumor (LCT) cultures inoculated with red cells infected withP. berghei. Intracellular parasites were present at 15 to 96 h after inoculation, being most numerous at 36 h. Most intracellular stages were rings, trophozoites, schizonts and merozoites; gametocytes were few in number and present only at 36 and 48 h. Intracellular parasites were normal in general morphology and staining characteristics at 15 to 48 h, but were abnormal after 72 h. Infected host cells exhibited progressive nuclear and cytoplasmic degenerative changes, which ultimately resulted in death of the cell. Uninfected cells appeared normal. The ability of parasites in LCT cultures to produce infections upon injection into mice was similar to that obtained with control cultures without LCT cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02462968
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