ISSN:
1432-1211
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Abstract The immune responses of inbred mice to the terpolymers poly(glu48-Iys32 ala20) GLA20, poly(glu36lys24ala40) GLA40, and poly(glu24lys16ala60) GLA60 were studied. Antibody levels were measured with the homologous, as well as with the crossreacting polymers (glu60ala40) GA and (glu60lys40) GL. It was determined that the terpolymers consist of many determinants of varying immunogenic strengths which account for the dose dependency requirements for responsiveness as follows: mice ofH-2 haplotypesa, b, d, k, ands respond to ten and 100μg GLA20 and GLA40 and to one, ten, and 100 μg GLA60; mice ofH-2 haplotypesp, q, andr do not respond well to any concentration of GLA20 but respond well to 100 μg GLA40 and tenμg GLA60. That the congenic mice C3H.NB (H-2 p) and B10.R111 (H-2 r), having responder backgrounds of C3H (H-2 k) and C57BL/10 (H-2 b) mice, respectively, do not respond would suggest strongly that there is linkage of responsiveness toH-2 in the above strains. In addition, the responsiveness of AQR mice to GLA60 would map theIr gene(s) to the right of theK region, and most likely in theI region. The antibody against GLA20 was directed against GL. Responses of miceof H-2 haplotypesp, q, andr against GLA40 and GLA60 were directed predominantly against unique GLA determinants that were neither GA nor GL. Mice of the other respondingH-2 haplotypes (a, b, d, k, ands) produced antibody against these unique GLA specificities, as well as against GL and/or GA determinants. The importance of measuring responses with the homologous polymer is therefore demonstrated. It was postulated that the recognition of GLA20 at the T-cell level is via GLA determinants having a limited amount of alanine, which are different from those helical GLA determinants recognized in the polymers GLA40 and GLA60.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01563905
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