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  • 1980-1984  (4)
  • 1
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    Acrosin and the acrosome in human spermatogenesis (1983)
    Springer
    Details
    Publication Date: 1983-11-01
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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  • 2
    Electronic Resource
    Electronic Resource
    Acrosin and the acrosome in human spermatogenesis (1983)
    Springer
    Human genetics 〈Berlin〉 65 (1983), S. 61-67 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the indirect immunofluorescent staining technique, the developmental patterns of (pro)acrosin and the outer acrosomal membrane were studied in human spermatogenesis. Specific antibodies against purified acrosin and outer acrosomal membranes from boar spermatozoa were raised in the rabbit and were found to crossreact with (pro)acrosin and outer acrosomal membrane from human spermatogenic cells. It was concluded that (pro)acrosin as well as the molecules building up the outer acrosomal membrane have been highly conserved during mammalian evolution. In the course of human spermatogenesis (pro)acrosin as well as the outer acrosomal membrane first appear in the haploid spermatids; the fluorescent areas of the individual cells steadily increase during spermiogenesis. Staining for acrosin and the outer acrosomal membrane, respectively, was found in identical compartments of the spermatogenic cells in juxtaposition to the nucleus. Round-headed spermatozoa from an infertile patient did not stain for (pro)acrosin or outer acrosomal membrane. The lack of the acrosin system was further substantiated by the gelatin substrate film technique demonstrating the absence of a gelatinolytic protease in round-headed spermatozoa. Hence, round-headed spermatozoa lack the acrosome with its constituent membrane proteins and the acrosin system housed by the acrosome of normal spermatozoa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285030
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  • 3
    Electronic Resource
    Electronic Resource
    A two-dimensional analysis of variance for the simultaneous quantitative determination of estradiol and its 4-14C isotopically labelled analogue with selected ion monitoring (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 8 (1981), S. 260-264 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Estradiol-17β and [4-14C]estradiol-17β in total mass amounts of 0, 10 and 20 pg per analysis in the presence of 1000 pg [2H8]estradiol as an internal standard were simultaneously quantitatively determined by gas chromatography mass spectrometry using the selected ion monitoring technique. The experiment (a nested three way analysis of variance) was designed to investigate the two-dimensional error structure which facilitates the study and comparison of the variances: (1) the variance originating in the device, including gas chromatograph, mass spectrometer and computer system with its interfaces; (2) the variance originating from titration, pipetting and derivatization of the samples. The study revealed that in this concentration range the variance due to sample preparation is smaller than that due to the device. The deviations due to the device were shown to have a two-dimensional normal distribution.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200080607
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  • 4
    Electronic Resource
    Electronic Resource
    An application of gas chromatography mass spectrometry to the quantitation of substances and their labelled variants (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 273-276 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method is proposed for the quantitative estimation of a substance and its labelled analogue using the selected ion monitoring technique of gas chromatography mass spectrometry, in which estimation is achieved without a third labelled analogue as internal standard. In a first measurement the ratio of natural to labelled substance is estabolished. Then a known amount of either labelled or natural compound is added, the isotope ratio is determined a second time and from these two measurements the mass amounts can be calculated. When applied to the heptafluorobutyrates of androstenedione and progesterone about 10 pg of 14C labeled compounds can be determined in the presence of 1 ng of the natural substances.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200070610
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