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  • 1
    Electronic Resource
    Electronic Resource
    Physarum myosin light chain binds calcium (1980)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1980), S. 63-71 
    Details
    ISSN: 0886-1544
    Keywords: Physarum polycephalum ; myosin light chains ; polyacrylamide gel electrophoresis ; calcium ; cytoplasmic streaming ; actomyosin ATPase regulation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Myosin from the slime mold Physarum polycephalum contains three sizes of polypeptides: a heavy chain and two light chains, LC-1 and LC-2. Using a simple qualitative test for calcium binding by comparing electrophoretic migration of the polypeptides in sodium dodecy1 sulfate (SDS) acrylamide gels in the presence and absence of calcium, we have found that Physarum myosin light chain LC-2 migrates with an apparent molecular weight of 16,900 daltons in the presence of the metal ion chelator ethylene glycol bis (B-aminoethyl ether) N,N′-tetraacetic acid (EGTA). However, if calcium chloride is added to the sample prior to electrophoresis, the apparent molecular weight decreases to 16,100. Lanthanide and cadmium ions, but not magnesium, can substitute for calcium. Because the ionic radii of Ca2+, La3+, and Cd2+ are almost identical, we conclude that Physarum myosin LC-2 possesses a very size-specific binding site for calcium. Physarum myosin LC-1 and the heavy chain give no evidence for binding calcium by this test. Since cytoplasmic streaming in the plasmodium of Physarum requires calcium, our evidence indicates that the calcium-binding property of Physarum myosin LC-2 may be important in regulating the production of force by actomyosin in the ectoplasm. Unexpectedly, the myosin light chain in Physarum capable of binding calcium, LC-2, is the essential light chain, while LC-1 is a member of the regulatory class of myosin light chains [V. T. Nachmias, personal communication]. Until now, essential myosin light chains have not been shown to have high affinity divalent cation binding sites. This means a new version of the myosin-based model for actomyosin regulation by calcium may be required to explain cytoplasmic movement in Physarum, and perhaps in other motile systems involving cytoplasmic myosins as well.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970010106
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    Paper (German National Licenses)
    Fulltext
  • 2
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    Update on the TPC at triumf (1984)
    Elsevier
    Details
    Publication Date: 1984-09-01
    Print ISSN: 0167-5087
    Electronic ISSN: 1872-9606
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Published by Elsevier
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    PAPER CURRENT
  • 3
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    Limits on production of anomalous secondaries in deuteron-deuteron collisions at 7.9 GeV/c (1983)
    American Physical Society
    Details
    Publication Date: 1983-06-01
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
    Published by American Physical Society
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    PAPER CURRENT
  • 4
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    Construction and Initial Tests of the Time Projection Chamber at TRIUMF (1981)
    Institute of Physics
    Details
    Publication Date: 1981-04-01
    Print ISSN: 0031-8949
    Electronic ISSN: 1402-4896
    Topics: Physics
    Published by Institute of Physics
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    PAPER CURRENT
  • 5
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    Derivation of the collision probability between orbiting objects The lifetimes of Jupiter's outer moons (1981)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: A general form is derived for Opik's equations relating to the probability of collision between two orbiting objects to their orbital elements, and used to determine the collisional lifetime of the eight outer moons of Jupiter. The derivation is based on a concept of spatial density, or average number of objects found in a unit volume, and results in a set of equations that are easily applied to a variety of orbital collision problems. When applied to the outer satellites, which are all in irregular orbits, the equations predict a relatively long collisional lifetime for the four retrograde moons (about 270 billon years on the average) and a shorter time for the four posigrade moons (0.9 billion years). This short time is suggestive of a past collision history, and may account for the orbiting dust detected by Pioneers 10 and 11.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Icarus; 48; Oct. 198
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Collision avoidance in space (1980)
    In:  Other Sources
    Details
    Publication Date: 2011-08-17
    Description: Collisions in earth orbital space between operational payloads and various forms of space debris (nonoperational payloads, nonfunctional mission-related objects and fragments resulting from collisions and explosions) are discussed and possible means of avoiding them are considered. From 10,000 to 15,000 objects are estimated to be in earth orbital space, most of which represent spacecraft fragments and debris too small to be detected and tracked by earth-based sensors, and it is considered likely that some of them will be or have already been involved in direct collisions with the ever increasing number of operational satellites and space stations. Means of protecting proposed large space structures and smaller spacecraft from significant damage by larger space objects, particularly in the 400-4000 km altitude range where most debris occurs, include structural redundancy and the double shielding of sensitive components. Other means of collision avoidance are the collection or relocation of satellites, rocket bodies and other objects by the Space Shuttle, the prevention of explosions and the disposal of spent rocket parts by reentry. Finally, a management structure would be required to administer guidelines for the prevention and elimination of space debris.
    Keywords: ASTRONAUTICS (GENERAL)
    Type: IEEE Spectrum; 17; June 198
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
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    Ground radar detection of meteoroids in space (1980)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: A special test to lower the detection threshold for satellite fragments potentially dangerous to spacecraft was carried out by NORAD for NASA, using modified radar software. The Perimeter Acquisition Radar Attack Characterization System, a large, planar face, phased radar, operates at a nominal 430 MHz and produces 120 pulses per second, 45 of which were dedicated to search. In a time period of 8.4 hours of observations over three days, over 6000 objects were detected and tracked of which 37 were determined to have velocities greater than escape velocity. Six of these were larger objects with radar cross sections greater than 0.1 sq m and were probably orbiting satellites. A table gives the flux of both observed groups.
    Keywords: ASTRONOMY
    Type: Symposium on Solid particles in the solar system; Aug 27, 1979 - Aug 30, 1979; Ottawa; Canada
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Sources of orbital debris and the projected environment for future spacecraft (1980)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: The major source of the nearly 5000 objects currently observed orbiting the earth is from rocket explosions. These explosions have almost certainly produced an even larger unobserved population. If the current trend continues, collisions between orbiting fragments and other space objects could be frequent. By the year 2000 satellite fragmentation by hypervelocity collisions could become the major source of earth orbiting objects, resulting in a self propagating debris belt. The flux within this belt could exceed the meteoroid flux, affecting future spacecraft design.
    Keywords: ASTRONAUTICS (GENERAL)
    Type: AIAA PAPER 80-0855 , International Meeting and Technical Display on Global Technology 2000; May 06, 1980 - May 08, 1980; Baltimore, MD
    Format: text
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    NASA TECHNICAL REPORTS
  • 9
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    Summary of Orbital Debris Workshop (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-27
    Description: An Orbital Debris Workshop was conducted in July 1982. The working groups established were related to measurements of large particles, modeling of large particles, measurements of small particles, spacecraft hazard and shielding requirements, and space object management. The results of the Orbital Debris Workshop reaffirm the need for research to better understand the character of orbital debris, its effects on future spacecraft, and the related requirements for policy. A clear charter is required for this research to receive the necessary support, focus, and coordination. It was recommended that NASA assume the role of lead agency. The first task is to develop an overall plan with both Department of Defense and the North American Aerospace Defense Command participation.
    Keywords: ASTRONAUTICS (GENERAL)
    Type: IAF PAPER 82-254
    Format: text
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    NASA TECHNICAL REPORTS
  • 10
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    Man-made space debris - Does it restrict free access to space (1981)
    In:  Other Sources
    Details
    Publication Date: 2019-07-27
    Description: Consideration is given to the hazards posed by existing and future man-made space debris to spacecraft operations. The components of the hazard are identified as those fragments resulting from spacecraft explosions and spent stages which can be tracked, those fragments which are too small to be tracked at their present distances, and future debris, which, if present trends in spacecraft design and operation continue, may lead to an unacceptably high probability of collision with operational spacecraft within a decade. It is argued that a coordinated effort must be undertaken by all space users to evaluate means of space debris control in order to allow for the future unrestricted use of near-earth space. A plan for immediate action to forestall the space debris problem by activities in the areas of education, debris monitoring and collection technology, space vehicle design, space operational procedures and practices and space policies and treaties is proposed.
    Keywords: ASTRONAUTICS (GENERAL)
    Type: IAF PAPER 81-256 , International Astronautical Congress; Sept. 6-12, 1981; Rome; Italy
    Format: text
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    NASA TECHNICAL REPORTS
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