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  • Cell & Developmental Biology  (3)
  • 1980-1984  (3)
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Keywords
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Binding of isolated 3T3 surface membranes to growing 3T3 cells and their effect on cell growth (1982)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 20 (1982), S. 81-93 
    Details
    ISSN: 0730-2312
    Keywords: 3T3 cells ; 3T3 surface membranes ; growth inhibition ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have quantitated by autoradiography the binding of [125I]labeled 3T3 plasma membrane fragments to 3T3 cells growing on the surface of plastic dishes; ie, the same conditions in which these membranes specifically arrest the growth of 3T3 cells early in the G1 phase of the cell cycle. We have been able to demonstrate that binding of membranes to cells is coincidental with the expression of the growth inhibitory activity of protein(s) present in the membrane fragments. Treatments that reduce binding (heat denaturation of the membranes or culture in the presence of high scrum) also reduce growth inhibitory activity. [125I]labeled membranes bound to cells are located primarily on the cell surface (as determined by electron microscope autoradiography) and are exchangeable with unlabeled membranes. We conclude that binding of membranes to cells is necessary but may not be sufficient for the expression of the growth inhibitory activity of these membranes. This approach provides information not only on the average level of binding of membranes to cells, but also provides a quantitative assessment of the variation of the level of membrane to cell binding between different cells in the population.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240200109
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Growth inhibitory protein(s) in the 3T3 cell plasma membrane. Partial purification and dissociation of growth inhibitory events from inhibition of amino acid transport (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 108 (1981), S. 35-45 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It has been reported previously that a plasma membrane-enriched fraction from 3T3 cells arrests the growth of sparse 3T3 cells early in the G1 phase of growth (Whittenberger et al., ′78, ′79). Addition of membranes to sparse 3T3 cells also results in a decrease of the rate of transport of αaminoisobutyric acid (Lieberman et al., ′79). We have partially purified the growth-inhibitory proteins from plasma membrane of 3T3 cells. These growth-inhibitory proteins behave as intrinsic membrane proteins and do not require membrane lipids for activity. The partially purified proteins arrest cell growth without affecting the rate of α-aminoisobutyric transport; thus, inhibition of both transport and cell growth are not obligatorily coupled events.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041080106
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  • 3
    Electronic Resource
    Electronic Resource
    Mitogenic proteins of the 3T3 plasma membrane (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 114 (1983), S. 73-76 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A mitogenic factor from 3T3 plasma membranes has been identified and partially characterized. The factor appears to be a peripheral membrane protein that can be released by mild trypsin, chymotrypsin, or plasmin treatment. This component is sensitive to heat and acid, and has a molecular weight in the range of 150,000-200,000 daltons as determined by gel filtration. A similar mitogenic activity has also been found on the membranes of both SV-40-transformed 3T3 cells and human fibroblasts. The factor appears to be distinct from all previously described mitogenic components.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041140112
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