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  • 1
    Electronic Resource
    Electronic Resource
    cGMP stimulates active K+ uptake in rat submandibular slices (1983)
    Springer
    Cellular and molecular life sciences 39 (1983), S. 362-364 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The 8-bromo derivative of cGMP was found to stimulate the ouabain-sensitive uptake of K+ and to reduce the net release of K+ induced by acetylcholine in rat submandibular gland slices incubated in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01963123
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  • 2
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    cGMP stimulates active K+ uptake in rat submandibular slices (1983)
    Springer
    Details
    Publication Date: 1983-04-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Structural and functional membrane polarity in cultured monolayers of MDCK cells (1980)
    Springer
    The journal of membrane biology 52 (1980), S. 147-159 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary MDCK cells form monolayers which have many of the properties usually found in transporting epithelia. The present article is devoted to the study of the structural and functional polarization of MDCK cells, which is one of the central features of transporting epithelia. The results show: (i) that MDCK monolayers transport 2.6 μmol hr−1 cm−2 of sodium in the apical to basolateral direction; (ii) the passive flux of this ion is relatively large (20.3 mole hr−1 cm−2), which is a characteristic of leaky epithelia; (iii) a large fraction of the penetration of sodium into the cells proceeds through an amiloride-sensitive channel, and the exit is operated mainly by a ouabain-sensitive pump; (iv) the net transport of sodium from the apical to the basolateral side agrees with the asymmetric labeling of the pumps with3H-ouabain; (v) this asymmetric labeling agrees, in turn, with a higher concentration of intramembrane particles (IMPs) in freeze-fracture replicas of the basolateral side of the plasma membrane; (vi) the structural polarization of confluent MDCK cells is also revealed by the location of microvilli, occluding junctions, and pinocytotic vesicles; and (vii) the presence of a continuous ring formed by actin microfilaments visualized by immunofluorescence under the lateral aspect of the plasma membrane that may be related to the distribution of the occluding junctions, which act as barriers separating apical from basolateral membrane components.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869120
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  • 4
    Electronic Resource
    Electronic Resource
    Occluding junctions in a cultured transporting epithelium: Structural and functional heterogeneity (1980)
    Springer
    The journal of membrane biology 53 (1980), S. 19-32 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary MDCK cells (epithelioid of renal origin) form monolayers which are structurally and functionally similar to transporting epithelia. One of these similarities is the ability to form occluding junctions and act as permeability barriers. This article studies the junctions of MDCK monolayers formed on a permeable and transparent support (a disk of nylon cloth coated with collagen) by combining two different approaches: (i)Scanning of the electric field: the disk is mounted as a flat sheet between two Lucite chambers and pulses of 20–50 μA cm−2 are passed across. The apical surface of the monolayer is then scanned with a microelectrode to detect those points where the current is flowing. This shows that the occluding junctions of this preparation are not homogeneous, but contain long segments of high resistance, intercalated with sites of high conductance. (ii)Freeze fracture electron microscopy: the junctions are composed of regions of eight to ten strands intercalated with others where the strands are reduced to one or two ridges. The sites of high conductance may correspond to those segments where the number of junctional strands is reduced to 1 or 2. It is concluded that the occluding junctions of MDCK monolayers are functionally and morphologically heterogeneous, with “tight” regions intermixed with “leaky” ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871169
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  • 5
    Electronic Resource
    Electronic Resource
    Hapten specific helper T cells restricted by theI-E(C) subregion of the MHC (1980)
    Springer
    Immunogenetics 10 (1980), S. 299-303 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01561578
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on cellulase production by Clostridium thermocellum (1980)
    Springer
    Applied microbiology and biotechnology 9 (1980), S. 189-197 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Clostridium thermocellum ATCC 27405 (and its improved cellulase-producing mutant, AS-39) is an anaerobic thermophile that produces endo-β-glucanase and exo-β-glucanase when grown on cellobiose or cellulose as major carbon source (Shinmyo et al. 1979). The site of cellulase accumulation was at least 95% extracellular. Optimum conditions for endo-β-glucanase production in flasks included 1% (w/v) cellobiose, 0.2% (w/v) urea as a nitrogen source, 0.1 M morpholinopropane-sulfonic acid buffer, an initial pH of 7.4, and a yeast extract concentration of 0.6% (w/v). An improved medium (GS medium) was devised for future studies. Xylan was degraded by an extracellular enzyme (s) produced during cultivation on cellobiose, although C. thermocellum does not grow on xylan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00504485
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation and properties of AgInS2 and AgInSe2 Single crystals and of the Quaternary alloys AgInSe2(1−x)S2x (1983)
    Springer
    Il nuovo cimento della Società Italiana di Fisica 2 (1983), S. 1687-1694 
    Details
    ISSN: 0392-6737
    Keywords: Methods of crystal growth and purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Description / Table of Contents: Riassunto Sono stati preparati cristalli singoli di AgInS2 e AgInSe2 mediante trasporto chimico con iodio e con il metodo di Bridgman. Sono state effecttuate anche sintesi delle leghe quaternarie AgInSe2(1−x)Se2x. Le separazioni delle bande di valenza piú alte del AgInS2 ortorombico sono state determinate dallo spettro di fotoconduttività. Si presentano i parametri del reticolo, la banda proibita, la suscettività magnetica e il punto di fusione di tutti i materiali.
    Abstract: Резюме Приготовляются монокристаллы AgInS2 и AgInSe2, исполъзуя метод транспорта иода и метод Бридгмана. Также проводится синтез четярехкомпонентнях сплавов AgInSe2(1−x)S2x. Из спектра фотопроводимости определяются расцепления верхних валентных зон в орторомбическом AgInS2. Приводятся параметры рещетки, щирина запрещенной зоны, магнитная воспримчивостъ и точка плавления для всех соединений.
    Notes: Summary AgInS2 and AgInSe2 single crystals have been prepared by chemical transport with iodine and by the Bridgman method. Syntheses of the quaternary alloys AgInSe2(1−x)S2x have also been carried out. The splittings of the uppermost valence bands in orthorhombic AgInS2 have been determined from the photoconductivity spectrum. The lattice parameters, the energy gap, the magnetic suceptibility and the melting point of all the materials are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02457851
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  • 8
    Electronic Resource
    Electronic Resource
    Correlation of hardness and microstructure in unoriented lamellar polyethylene (1983)
    Springer
    Journal of materials science 18 (1983), S. 1077-1082 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The stress-strain diagrams of a series of melt-crystallized polyethylene samples with a varying number of chain defects have been investigated. The elastic modulus,E, and the surface hardness,MH, markedly decrease with increasing number of defects. The mechanical behaviour of the lamellar structure of PE modulated by a major exclusion of chain defects from the crystals is discussed in the light of Takayanagi's two-phase model. The data suggest thatE is very sensitive to the fraction of tight crystalline bridges between lamellae. The correlation found betweenE andMH emphasizes, in addition, the different and complementary role played by the amorphous layer in each mechanical test. In the former case one measures the elastic deformation of the layer reinforced by tie molecules. In the latter test the plastic deformation under compression of the lamellae sandwiched between noncrystalline layers is contemplated. In both cases the influence of the number of defects drastically affects the nature of the crystalline lamellae and surface layer and consequently substantially modifies both types of properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00551976
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative measurement by radioimmunoassay of the brain microtubule-associated protein MAP2 (1981)
    Springer
    Molecular and cellular biochemistry 37 (1981), S. 185-189 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The presence of the microtubule-associated protein (MAP2) in the brain of several species has been investigated by SDS-gel electrophoresis and by radioimmunoassay. This assay had a sensitivity of approx. 10 ng and it was capable of measuring the protein either in purified microtubules or in crude brain extracts. As determined with this radioimmunoassay, MAP2 accounted for about 10% of the porcine brain microtubule protein and 1% of the protein from a brain extract. Taking porcine MAP2 as a reference, we have detected polypeptides with the same electrophoretic mobility in brain microtubules from cow, sheep, rat and chicken. Nevertheless, the MAP2 from these species showed a variable degree of immunoreactivity. Bovine MAP2 appeared closely related to the porcine protein whereas the rat antigen showed low cross-reaction and chicken MAP2 appeared immunologically unrelated to porcine MAP2. Our results suggest a higher variability of the MAP2 sequences as compared to that reported by other authors for the brain microtubule protein, tubulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02354887
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  • 10
    Electronic Resource
    Electronic Resource
    Podophyllotoxin poisoning of microtubules at steady-state: effect of substoichiometric and superstoichiometric concentrations of drug (1982)
    Springer
    Molecular and cellular biochemistry 45 (1982), S. 3-11 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Depolymerization kinetics of microtubules assembled to steady-state by pod ophyllotoxin treatment show a dose-dependent effect of this mitotic poison on the net rate of microtubule disassembly. Pulse-chase experiments with microtubules at steady-state indicate that the depolymerization effect induced by superstoichiometric concentrations of podophyllotoxin relative to tubulin is polar and time-dependent, i.e. the rate of tubulin loss decreases along with the time of treatment in the presence of the drug. Under these conditions the rate of microtubule disassembly is much faster than one could expect from a unique effect of drug-tubulin complex on the microtubule assembly end. Podophyllotoxin-tubulin complex is not able to induce active depolymerization of microtubules, while free podophyllotoxin is. These results are consistent with the hypothesis that this drug acts on the microtubule assembly-disassembly process by two different mechanisms: 1) as a free drug, it actively promotes polar depolymerization of microtubules, and 2) as a drug-tubulin complex, it retards the addition of subunits into the microtubule ends.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283158
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