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  • 1980-1984  (3)
  • 1970-1974  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Effects of Carbon Source, Gibberellins and Ethylene on Wall-Bound Invertase Activity in Callus of Convolvulus arvensis (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 30 (1974), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The growth rate of Convolvulus callus on a sucrose-containing medium appeared to be largely independent of the activity of cell wall invertase. The increase of this enzyme activity which occurs upon subculturing was unaffected within the first 24 h by the presence of the substrate sucrose. Neither substitution by glucose or fructose, nor complete deletion of the carbon source had any effect.Gibberellins apparently were not involved in the initiation and/or control of the increase of wall-bound invertase activity occurring upon subculturing. Exogenous ethylene was unable to mimick this effect of subculturing but when applied immediately after subculturing it had a synergistic effect on the increase of invertase. Inhibition occurred, however, when exposure to ethylene was delayed until after 24 h of incubation. These findings suggest a role of ethylene in the control of wall-bound invertase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1974.tb03667.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    α-Glucosidase Activity Located at the Cell Surface in Callus of Convolvulus arvensis (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 25 (1971), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cell wall preparations of Convolvulus callus were found to contain α-glucosidase activity, the bulk of which could be solubilized by solutions of high ionic strength. Callus tissue incubated in 0.5 M KC1 released α-glucosidase activity into the washing medium as distinct from tissue incubated in 1.0 M sorbitol. The wall-bound activity of KCl-treated tissue was found to be less than that of sorbitol-treated tissue, while the difference between both activities proved to be equal to the enzyme activity found in the washing medium of KCl-treated tissue. Since no trace of cell leakage was observed, it is concluded that α-glucosidase activity is located at the cell surface. The level of this surface-located enzyme was not affected by the presence of maltose in the nutrient medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1971.tb01438.x
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  • 3
    Electronic Resource
    Electronic Resource
    Wall-bound Invertase Activity in Convolvulus Callus: Increase after Subculturing, and Paradoxical Effects of Actinomycin D, Cycloheximide, and Thienylalanine (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 26 (1972), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The wall-bound invertase activity increased 3.3-fold upon transfer of fragments of Convolvulus callus to fresh solid nutrient medium and 7.7-fold upon transfer to liquid nutrient medium. Addition of actinomycin D, cycloheximide or the amino acid analogue thienylalanine brought about a further stimulation of the invertase content of the cell walls. The rise of wall-bound invertase activity was not due to redistribution of invertase activity between cytoplasm and cell walls, and appeared to be dependent on metabolic energy.An equation is presented to calculate the half-life of enzymes from their time-course. Applied on the time-courses of wall-bound invertase activity, a half-life of about 12 h was obtained in callus transferred to fresh solid medium and of about 5.4 h in tissue transferred to liquid medium. It is argued that the increase of invertase content of the cell walls is due to an enhanced rate of invertase synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1972.tb01123.x
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  • 4
    Electronic Resource
    Electronic Resource
    Growth and cell wall composition of glucose-limited bean cells (Phaseolus vulgaris L.) (1982)
    Springer
    Plant cell reports 1 (1982), S. 131-134 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glucose-limited bean cells (Phaseolus vulgaris L.) were grown in a modified bacterial fermentor at a constant pH of 4.8. The cultures were kept in steady state at different specific growth rates varying from 0.00216 h−1 to 0.0106 h−1. Culture conditions are described that are needed to start a continuous culture. First, it was essential to use log-phase cells as starting material. Second, it was important to increase the dilution rate gradually, otherwise cells in the culture aggregated. Cells grown at the highest dilution rate employed contained twice as much protein per gram dry weight as cells grown at the lowest dilution rate. The composition of the cell walls also varied with the dilution rate in contrast to their relatively constant composition when grown in batch culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272371
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  • 5
    Electronic Resource
    Electronic Resource
    Changing cell-wall compositions in hypocotyls of dark-grown bean seedlings (1980)
    Springer
    Planta 149 (1980), S. 209-212 
    Details
    ISSN: 1432-2048
    Keywords: Cell elongation ; Cell wall ; Glucan ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyls of dark-grown 6-day-old seedlings of Phaseolus vulgaris L. proved to be sufficiently homogeneous to permit studies relating the rate of cell elongation to the composition of the primary cell walls. Whereas the levels of cellulose and uronic acids remained practically constant during and after cell extension, all other components showed major or minor changes. Cell-wall protein, as such, decreased by more than 50%, but indications are that hydroxyproline-rich glycoprotein increased with a decreasing rate of cell elongation, concomitant with a rise in the degree of arabinosylation of wall-bound hydroxyproline. As cell elongation slowed down, non-cellulosic glucose accumulated, presumably in the form of a β-(1–4)glucan closely associated with cellulose. These findings confirm the notion that the primary cell wall is a highly dynamic structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384555
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  • 6
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    Changing cell-wall compositions in hypocotyls of dark-grown bean seedlings (1980)
    Springer
    Details
    Publication Date: 1980-08-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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