ISSN:
1040-0397
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A method using sodium amalgam electrodes has been developed to study the effect of protein on activity measurements and residual liquid junction potential of sodium in protein-containing solutions. It was tested for reliability by application to aqueous sodium chloride solutions before it was applied to protein-containing solutions. The experimentally determined activity coefficients are in good agreement with the activity coefficients for aqueous sodium chloride solutions reported in literature. Therefore, we used this method to measure the activity of NaCl solutions containing varying concentrations of bovine serum albumin (BSA). The electrode slope of the electrode responses in a protein-containing medium does not differ from that of pure aqueous solutions. A functional relationship has been established between the concentration of protein and the percent bias in the measurements. Measurements of electromotive force made in protein-containing solutions do not show any evidence of protein effect on liquid junction potential.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140030209
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