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  • 1
    Electronic Resource
    Electronic Resource
    Interaction of ethylene and CO2 (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 73 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Low levels of carbon dioxide regulate ethylene synthesis and higher levels inhibit ethylene action, but the mechanism for these effects is not known.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb00623.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of 2,5-norbornadiene on abscission and ethylene production in citrus leaf explants (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 63 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Citrus (Citrus sinensis L. Osbeck) leaf explants completely abscise within 48 h when exposed to saturating amounts of ethylene at 25°C. When 2,5-norbornadiene was added, 2000 μl 1−1 reduced abscission of explants also exposed to 2 μl 1−1 of ethylene to the level of the control, and 8000 μl 1−1 reduced abscission in explants exposed to 10 μl 1−1 of ethylene to the level of the control, but abscission was complete when 1 000 μl 1−1 of ethylene was used in the presence of 8 000 μl 1−1 of 2,5-norbornadiene. When explants were exposed to 2 μl 1−1 of ethylene, 2000 μl 1−1 of 2,5-norbornadiene prevented abscission if applied up to 10 h after exposure to ethylene. After 18 h, applied 2,5-norbornadiene had little effect on abscission at 48 h. A Lineweaver-Burk plot gave a 1/2 maximum value of 0.12 μl 1−1 for ethylene on abscission, 2,5-Norbornadiene gave competitive kinetics with respect to ethylene with a K1 value of approximately 120 μl 1−1 of 2,5-norbornadiene. The presence of 2,5norbornadiene stimulated ethylene production, which progressively increased as the 2,5-norbornadiene concentration was increased from 250 to 8 000 μl 1−1 2,5-Norbornadiene also suppressed the induction of cellulase and polygalacturonase by ethylene. Together, 2,5-norbornadiene and 2,4-dichlorophenoxyacetic acid were more effective than either alone in reducing abscission. 2,5-Norbornadiene also was effective in preventing the reduction of indole-3-acetic acid transport induced by ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb02828.x
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  • 3
    Electronic Resource
    Electronic Resource
    Ethylene binding changes in apple and morning glory during ripening and senescence (1989)
    Springer
    Journal of plant growth regulation 8 (1989), S. 37-44 
    Details
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ethylene binding sites were measured during fruit ripening and morning glory flower senescence. Little change in ethylene binding was noted during these developmental stages, except a slight decline during the later stages of fruit ripening or flower senescence. The concentration of ethylene required to achieve 50% saturation of the binding sites was 0.14 μl/liter for both apple pulp and morning glory flowers. Ethylene binding sites were calculated to be 3.2×10−11 moles/kg and 3.8×10−9 moles/kg in apple and morning glory, respectively. It does not appear that changes seen in ethylene sensitivity during fruit ripening can be readily ascribed to changes in the number of ethylene binding sites in the tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02024924
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  • 4
    Electronic Resource
    Electronic Resource
    Competition of unsaturated compounds with ethylene for binding and action in plants (1988)
    Springer
    Plant growth regulation 7 (1988), S. 181-191 
    Details
    ISSN: 1573-5087
    Keywords: Ethylene ; ethylene binding ; mung bean extract ; tobacco leaves ; banana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The compounds 2,5-norbornadiene, cyclopentadiene, furan, pyrrole, thiophene, 1-methylpyrrole, dicyclopentadiene, methylcyclopentadiene (dimer), and cycloheptatriene have been tested for competition with ethylene for binding and for biological activity using banana fruit. In addition, acetylene, allene, and 1,3-butadiene were tested. All of these compounds competed with ethylene for binding in vitro in a Triton X-100 extract of mung bean sprouts and in vivo in tobacco leaves. Only acetylene, allene, and furan are active in giving an ethylene response in banana. the others all suppress the ethylene response. Only acetylene and allene stimulate ethylene synthesis. Most of the compounds diffuse away from the binding site upon exposure to air.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028240
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  • 5
    Electronic Resource
    Electronic Resource
    Binding of ethylene analogs and cyclic olefins to a Triton X-100 extract from plants: Comparison with in vivo activities (1986)
    Springer
    Plant growth regulation 4 (1986), S. 13-21 
    Details
    ISSN: 1573-5087
    Keywords: Ethylene binding ; ethylene action in vitro ; ethylene analogs ; cyclic olefins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The binding constants of various olefins were determined with a Triton X-100 extract of mung bean sprouts. The olefins tested included compounds that have been reported to induce an ethylene response in vivo as well as olefins reported to block the ethylene response. Both types of compounds were bound by the Triton X-100 extract, and the binding constants in vitro were usually considerably lower than those obtained in in vivo studies as measured by gas phase concentrations. Increased solubility due to Triton X-100 solution appears to be partly responsible. The in vitro binding order of compounds reported to induce an ethylene response was similar to their order of in vivo activity. Also, the compounds which gave an anti-ethylene response in vivo bound to the extract in approximately the same order as their in vivo effectiveness. These results suggest that binding of olefins is not the only factor necessary for an ethylene response. Although binding is necessary for activity, another factor must be involved after binding, and this may be π-acceptance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025345
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  • 6
    Electronic Resource
    Electronic Resource
    Ethylene-binding characteristics in Phaseolus, Citrus, and Ligustrum plants (1986)
    Springer
    Plant growth regulation 4 (1986), S. 43-54 
    Details
    ISSN: 1573-5087
    Keywords: Phaseolus ; Citrus ; Ligustrum plants ; Ethylene binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A number of plants were tested for their ability to bind ethylene and the number of binding sites present in each was calculated. Primary leaves of laboratory-grown beans (Phaseolus vulgaris) bound 140 dpm/g fwt (1794 dpm/g dry wt) when exposed to 1.0 μCi/1 of [14C]ethylene (110 ci/mol). Phytotron-grown leaves were less succulent but only bound 90 dpm/g fwt (1046 dpm/g dry wt). Bean roots bound 30 dpm/g fwt. Citrus and Ligustrum bound 207 and 240 dpm/g fwt, respectively. The time required to achieve equilibrium of leaves with the gas phase was 15 min for bean, 30 min for Citrus, and 30–60 min for Ligustrum. The time for 1/2 of the bound ethylene to diffuse out of the leaves was 20 min for bean, 10 min for Citrus, and 30 min for Ligustrum. The amount of ethylene needed to occupy 1/2 of the binding sites was obtained from Scatchard plots. This value (Kd) was 0.2 μl/1 for bean, 0.15 for Citrus, and 0.31 for Ligustrum. The quantity of binding sites in the tissues was 2.0×10-9 mol of binding sites/kg tissue for bean leaves, 5.7×10-9 for Citrus leaves, and 6.8×10-9 for Ligustrum. Pretreatment with indoleacetic acid (IAA), ehtylene, and cycloheximide (1 mg/1) had little effect on the level of ethylene-binding sites in Citrus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025348
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