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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of α-tubulin genes from the human malaria parasite, Plasmodium falciparum: sequence analysis of α-tubulin (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: As a step towards indentifying exploitable differences between host and parasite at the molecular level, we have isolated and sequenced genomic clones encompassing an entire α-tubulin gene (designated α-tubulin I) from the human malaria parasite, Plasmodium falciparum. The gene, which contains two introns, encodes a product with a predicted length of 453 amino acid residues (50.3 kD). The protein sequence shows a high degree of homology to other α-tubulins, particularly that of the coccidian parasite, Toxoplasma gondii (94%), whose gene carries introns in identical positions. Only one copy of the α-tubulin I gene itself was found, although a second gene designated α-II was also identified which is closely related but which differs at both the nucleotide and amino acid sequence levels. The α-I and β-tubulin genes were found to reside on different chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00136.x
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning of a β-tubulin gene from Plasmodium falciparum (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We describe the isolation and characterization of a gene for β-tubulin from the malaria parasite, Plasmodium falciparum. This organism appears to contain a single gene encoding β-tubulin. A single transcript from this gene can be detected in the total RNA of the parasite's asexual blood stages. The complete sequence for the gene has been elucidated. It has two introns, one of which has a position identical to that of a related parasite, Toxoplasma gondii. The gene shows the usual preference for codons with A or T in the third position. The predicted amino acid sequence is compared with that of T. gondii and the human host. Further comparisons between these and fungal sequences of β-tubulins resistant to benomyl, a drug binding this protein, highlight differences that could be exploited in the development of parasite-specific anti-tubulin drugs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00137.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of nitrogen form on the growth and nitrate concentration of lettuce (1986)
    Springer
    Plant and soil 94 (1986), S. 3-16 
    Details
    ISSN: 1573-5036
    Keywords: Ammonium ; Critical nitrate concentration ; Lettuce ; Midrib ; Nitrification inhibitor ; Nitrapyrin ; Sap test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A pot experiment with lettuce involving three N forms each at six application levels, showed that lettuce can be grown satisfactorily with a very low nitrate content when supplied with ammonium sulphate and a nitrification inhibitor. For plants growing on nitrate N, the optimum midrib sap nitrate concentration as maturity approached was about 1400 mg/1 NO3-N. Large losses of mineral N were observed from the peat medium, even in the absence of plants. A relationship is presented which would enable a lettuce grower to estimate whole-shoot nitrate concentration from a quick test of midrib sapi.e. NO3-N (mg/kg in fresh shoot) =0.14×NO3-N (mg/l in sap). Tipburn was worst at intermediate levels of applied N, and was less serious with pure ammonium nutrition than with nitrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380586
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  • 4
    Electronic Resource
    Electronic Resource
    Derivation of critical nutrient concentrations for growth rate from data from field experiments (1988)
    Springer
    Plant and soil 109 (1988), S. 159-169 
    Details
    ISSN: 1573-5036
    Keywords: Brassica oleracea vargemmifera ; Brussels sprout ; model ; nitrate ; nitrogen ; sap ; simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Present methods for determining critical nutrient concentrations in plants and soils are unsatisfactory if concentrations change with time while the crop is growing. In such cases the critical concentration can only be applied in relation to growth rate at any given moment. For interpreting field experiments this introduces considerable difficulties: two possible approaches to these problems are suggested, one of which uses a simple simulation model. Results from a Brussels sprout nitrogen experiment are used to show how, using this approach, a single critical sap nitrate concentration (380 mg NO3 N.l−1 for 95% of potential growth rate) may serve to explain the results at all growth stages in three seasons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02202080
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  • 5
    Electronic Resource
    Electronic Resource
    A pump/leak/buffer model for plant nitrate uptake (1989)
    Springer
    Plant and soil 114 (1989), S. 139-141 
    Details
    ISSN: 1573-5036
    Keywords: diurnal ; efflux ; lag ; nitrogen assimilation ; phase shift ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A simple simulation model is described to account for the rates at which plants take up nitrate and reduce it to protein. It is based on the pump and leak principle, with the pump working at a constant rate per unit sap volume provided that there is an adequate concentration of nitrate at the root surface. The rate of leakage is assumed to be proportional to the concentration difference between the inside and the outside of the plant. Nitrogen is removed from the plant nitrate pool (the ‘buffer’) at a constant fraction of the photosynthesis rate. When applied to data for the diurnal variation in nitrate uptake by ryegrass, the model predicts an uptake pattern similar to that actually observed, with a time lag of about 5 hours between photosynthesis and uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02203092
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  • 6
    Electronic Resource
    Electronic Resource
    The sulphate-S/total S ratio in plants as an index of their sulphur status (1986)
    Springer
    Plant and soil 91 (1986), S. 61-71 
    Details
    ISSN: 1573-5036
    Keywords: Critical concentration ; Plant analysis ; Sulphate sulphur ; Sulphate/total sulphur ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Several authors are advocating the use of the SO4−S/total S ratio in the plant as the best index of S status. We have traced the arguments put forward in support of this index, and we show that they are based either on unfair comparisons with other indices, such as SO4−S or total S alone, or inappropriate statistical treatment. The SO4−S/total S index has two fundamental disadvantages compared with SO4−S or total S alone: (1) the numerator (SO4−S) is the major variable in the denominator, so the ratio is likely to be less sensitive than either of the measurements alone; (2) its determination involves twice as much analytical work as either measurement alone. Examination of some of the source references indicates that SO4−S by itself is the most satisfactory S index. Whenever whole plants are analysed, any index which includes organic S is subject to variation due to tissue age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181819
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  • 7
    Electronic Resource
    Electronic Resource
    Role of plasminogen, plasmin, and plasminogen activators in the migration of fibroblasts into plasma clots (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 132 (1987), S. 501-508 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Human diploid fibroblasts were seeded onto or into plasma clots and different aspects of cell adhesion and migration were measured. The roles of plasminogen activators and plasmin were studied by either the removal of plasminogen from plasma prior to clotting or by the addition of 10 mM ∊-aminocaproic acid, which brings about an inhibition of plasmin in this system. When cells were seeded onto the surface of plasma clots, rates of attachment, spreading, and migration were unaffected by plasminogen depletion or plasmin inhibition. In contrast, when cells were seeded into plasma clots, then, although the rates of cells spreading were unaffected, cell migration was abolished by plasminogen depletion or by plasmin inhibition. When cells were seeded onto the surface of plasma clots and the rate of migration into the clots was measured, there was an absolute requirement for plasmin activity; while fibroblasts migrated rapidly into the fibrin lattice of control clots, in the case of plasminogen-depleted clots, cells failed to penetrate the lattice. Focussing through a plasma clot revealed that fibroblasts do not migrate through the fibrin lattice but instead, localized areas of fibrinolysis are generated and cells migrate over the surface of the area of lysis.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041320312
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  • 8
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    The Parsons Steam Turbine (1985)
    Springer Nature
    Details
    Publication Date: 1985-04-01
    Print ISSN: 0036-8733
    Electronic ISSN: 1946-7087
    Topics: Biology , Natural Sciences in General , Physics
    Published by Springer Nature
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  • 9
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    Concept, operation and performance of the veeco VHC-120 high current ion implanter (1985)
    Elsevier
    Details
    Publication Date: 1985-01-01
    Print ISSN: 0168-583X
    Electronic ISSN: 1872-9584
    Topics: Physics
    Published by Elsevier
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  • 10
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    The Veeco 4840 automated implant system (1987)
    Elsevier
    Details
    Publication Date: 1987-01-01
    Print ISSN: 0168-583X
    Electronic ISSN: 1872-9584
    Topics: Physics
    Published by Elsevier
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