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  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the structural gene for class I pilin from Neisseria meningitidis: homologies with the pilE locus of Neisseria gonorrhoeae (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The nucleotide sequence has been determined for the expressed pilin (pilE) locus of Neisseria meningitidis strain C311 which produces class I pili that areantigenically and structurally similar to those of gonococci. The deduced amino acid sequence of the N. meningitidis pilE translation product contains a 7amino acid N-terminal pre-pilin leader sequence which is identical to that found in gonococcal pilin and which is characteristic of N-methylphenylalanine pili in general. The succeeding N-terminal 53 amino acids are identical to those found in the equivalent position in antigenically variant gonococcal pilins and confirm direct peptide sequencing of the amino-terminus of atleast one type of meningococcal pilin. Other regions that are conserved in variant pilin potypeptides from Neisseria gonorrhoeae are conserved at the aminoacid level in the class I meningococcal pilin but the coding DNA contains numerous base substitutions when compared with the equivalent gonococcal pH sequence. Sequences extending downstream for about 140bp on the 3′ side of the coding region for both pilin genes are only about 85% homologous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00073.x
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  • 2
    Electronic Resource
    Electronic Resource
    Constant-volume pressure measurements on BCC solid3He (1986)
    Springer
    Journal of low temperature physics 62 (1986), S. 67-109 
    Details
    ISSN: 1573-7357
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Measurements have been made of the pressure of the bcc phase of solid3He at molar volumes from 24.2 to 23.2 cm3 and at temperatures from 25 to 0.5 mK. The solid undergoes an unusual antiferromagnetic ordering at a temperature that depends strongly on the sample density. This ordering is due to the unusually large exchange interaction in solid3He, a factor of 104 stronger than the nuclear dipole-dipole interaction. The large latent heat associated with the first-order transition has constrained the size of the sample. The pressure of 0.005 cm3 of solid3He has been measured while the volume of the experimental cell was regulated to within 15 parts in 106. The results provide information on the magnitudes and molar volume dependences of the exchange rates, and suggest that a single physical process governs all aspects of the ordering transition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00681321
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  • 3
    Electronic Resource
    Electronic Resource
    Morphogenetic effects of 2,4-dichlorophenoxyacetic acid on pinto bean (Phaseolus vulgaris L.) leaf explants in vitro (1987)
    Springer
    Plant cell reports 6 (1987), S. 46-49 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Roots, callus and/or globular structures were produced on primary leaf and distal cotyledon explants of pinto bean (Phaseolus vulgaris L. cv. UI 114) cultured on semisolid MS medium with a wide range of 2,4-D concentrations (0.01 to 80 mg/L) with either 0 or 1.0 mg/L kinetin. Explants rooted at lower 2,4-D concentrations than at those favoring globule formation on callus, although roots, callus and globules often developed from the same explant. Isolated opaque green globular structures developed when callus initiated on media with 3 or more mg/L 2,4-D was subcultured in liquid MS + 30 mg/L 2,4-D. These structures multiplied with a fresh weight doubling time of 8–9 days in MS + 30 mg/L 2,4-D. Although this multiplicative behavior and opaque color were reminiscent of embryoids reported for other species, no cotyledons or roots were seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269737
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  • 4
    Electronic Resource
    Electronic Resource
    Hormone-free medium will support callus production and subsequent shoot regeneration from whole plant leaf explants in some sugarbeet (Beta vulgaris L.) populations (1989)
    Springer
    Plant cell reports 8 (1989), S. 222-225 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sugarbeet plants representing 14 of 16 germplasm sources (4 to 5 plants per source) produced callus from leaf disks on a hormone-free Murashige and Skoog based medium. Overall, 49.2% of explants from partially expanded leaves of whole plants initiated callus (53 of 74 plants tested), in an average time of 96.7 days. The time to callus was considerably longer than the 4–6 weeks observed when 1 mg/L N6-benzyladenine has been used in the medium. Shoots were regenerated on the hormone-free medium without subculture from callus of eight individual genotypes, representing 3 of the 14 populations that produced callus. Shoots produced by ‘Gartons White Knight’ and ‘L53’ appeared to be of somatic embryo origin. Rhizogenic calli were also produced by the same three populations that regenerated shoots. Significant differences among populations were found for frequency of root formation from leaf disks and time to callus. Variation among plants within a population was significant for four of the five traits examined. The results indicate the ease of hormone autonomization in sugarbeet, and should be of value in designing regeneration media for a wider range of beet germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00778538
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning and expression of penicillin acylase genes from overproducing strains of Escherichia coli and Bacillus megaterium (1987)
    Springer
    Applied microbiology and biotechnology 25 (1987), S. 372-378 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Penicillin acylase genes from Escherichia coli 194, of an overproducing mutant (194-3) of this strain, and of a similar overproducing mutant of Bacillus megaterium UN1 were cloned in E. coli DH1 on the plasmid vector pACYC184. The sizes of chromosomal DNA fragments essential for penicillin acylase production were found by Tn1000 mutagenesis and in vitro deletions to be between 2.2 and 2.5 kb in the case of both E. coli genes and between 2.3 and 2.7 kb in the case of the mutant Bacillus gene. Restriction mapping indicated substantial sequence differences between the E. coli and B. megaterium penicillin acylase genes. Enzyme production in E. coli recombinants from both overproducing mutants was found to be constitutive and higher than in the original strains. The Bacillus penicillin acylase was produced intracellularly in E. coli recombinants, which is in contrast to the normal extracellular production of this enzyme in B. megaterium. Recombinant plasmids containing penicillin acylase genes from either source were found to be unstable in the absence of selection pressure for retention of the vector.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252550
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  • 6
    Electronic Resource
    Electronic Resource
    Cloning of DNA sequences encoding theRsr I restriction-modification system ofRhodopseudomonas sphaeroides 630 (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 105-105 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01976002
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  • 7
    Electronic Resource
    Electronic Resource
    The temperature and pressure dependence of the normal fraction of superfluid3He-B (1985)
    Springer
    Journal of low temperature physics 61 (1985), S. 337-361 
    Details
    ISSN: 1573-7357
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Measurements have been made of the normal fluid fraction of superfluid3He-B at eight different pressures between 0 and 29.2 bar. The minimum temperatures were such that ρ n /ρ was reduced to less than 0.5%. The experiments, carried out independently at Cornell and Texas A&M Universities, used similar Andronikashvilli-type torsional oscillators. The results are qualitatively similar, but show significant differences due to the use of different temperature standards. Both sets of results are presented in tabular form. The inconsistencies in the temperature scales preclude the examination of the results for possible strong coupling effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00683691
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  • 8
    Electronic Resource
    Electronic Resource
    Plant allelochemicals and insect parasitoids effects of nicotine onCotesia congregata (say) (Hymenoptera: Braconidae) andHyposoter annulipes (Cresson) (Hymenoptera: Ichneumonidae) (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1319-1328 
    Details
    ISSN: 1573-1561
    Keywords: Plant allelochemical ; parasitoids ; three trophic level interactions ; nicotine ; Manduca sexta ; Lepidoptera ; Sphingidae ; Spodoptera frugiperda ; Noctuidae ; Cotesia congregata ; Hymenoptera ; Braconidae ; Hyposoter annulipes ; Ichneumonidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Parasitoids developing within tobacco hornworms or fall army-worms exhibit significant differences in development and survival depending on whether their hosts fed on nicotine-free or nicotine-containing diets. The effects of nicotine were more severe on the relatively less adapted parasitoid,H. annulipes than the specialist parasitoid,Cotesia congregata. Labeled alkaloid originally placed in herbivore diet was incorporated in several parasitoid tissues. These results suggest that interactions between plant allelochemicals and parasitoids should be considered in the development of theory on insect herbivory and plant defense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01012351
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  • 9
    Electronic Resource
    Electronic Resource
    Secondary structure of the human growth hormone releasing factor (GRF 1-29) by two-dimensional 1H-nmr spectroscopy (1988)
    New York : Wiley-Blackwell
    Biopolymers 27 (1988), S. 1897-1904 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The secondary structure of the human growth hormone releasing factor (GRF 1-29) in a solution mixture of 60% aqueous phosphate buffer:40% 2,2,2-trifluoroethanol-d3 has been investigated by two-dimensional 1H-nmr spectroscopy. Sequential resonance assignments and elements of secondary structure were obtained from phase-sensitive correlation spectroscopy, relayed coherence spectroscopy, and nuclear Overhauser spectroscopy experiments. The observation of a large number of α-amide and amide-amide interresidual nuclear Overhauser effect connectivities as well as the existence of 11 slowly exchanging amide protons indicates that the peptide adopts a well-defined secondary structure most likely constituted of a single long helix. This conclusion is consistent with the CD measurements.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360271204
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  • 10
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    Momentum transfer betweenHe3quasiparticles and surfaces: The effective viscosity of dilute solutions ofHe3inHe4 (1987)
    American Physical Society
    Details
    Publication Date: 1987-07-27
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
    Published by American Physical Society
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