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  • 1985-1989  (1,421)
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  • 1
    Electronic Resource
    Electronic Resource
    Reissner's fiber, massa caudalis and ampulla caudalis in the spinal cord of lamprey larvae (Geotria australis) (1987)
    Springer
    Cell & tissue research 247 (1987), S. 359-366 
    Details
    ISSN: 1432-0878
    Keywords: Reissner's fiber ; Massa caudalis ; Subcommissural organ ; Spinal cord ; Central canal ; Immunocytochemistry ; Lectin histochemistry ; Lamprey ; Geotria australis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcommissural organ (SCO), Reissner's fiber (RF) and its massa caudalis of lamprey larvae (Geotria australis) were investigated immunocytochemically by use of an antiserum raised against bovine RF as primary antibody. The affinities of RF and massa caudalis for Ricinus communis agglutinin I (RCA) with and without previous acid hydrolysis, concanavalin A (Con A), wheat-germ agglutinin (WGA), aldehyde fuchsin, and PAS reaction were also studied. SCO and massa caudalis were strongly immunoreactive, whereas RF proper was distinctly negative. RF did not react with Con A and RCA. Only the periphery of RF was WGA-positive. RCA showed affinity for RF only after acid hydrolysis. RF was homogeneously stained by the aldehyde-fuchsin and PAS-methods. At variance with RF proper, the periphery of the massa caudalis reacted with RCA without previous acid hydrolysis, but its core was WGA-positive and reacted with RCA only after hydrolysis. It is suggested that (i) RF has a coat of glycoproteins containing sialic acid as terminal residue, whereas the massa caudalis possesses a coat with galactose as terminal residue; (ii) in RF proper and the massa caudalis the spatial arrangement of glycoproteins might be different. Routine transmission electron-microscopic observations indicate that in larvae of Geotria australis an open communication exists between the ampulla caudalis and blood capillaries via large cavities or lacunae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218317
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  • 2
    Electronic Resource
    Electronic Resource
    Intercalation of polar organic compounds into tin(IV) hydrogenphosphate monohydrate (1985)
    s.l. : American Chemical Society
    Inorganic chemistry 24 (1985), S. 1187-1190 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00202a015
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  • 3
    Electronic Resource
    Electronic Resource
    Thermal studies on Ni(II), Cu(II) and Pd(II) complexes of 6-amino-5-formyluracil and its N-methyl derivatives (1987)
    Springer
    Journal of thermal analysis and calorimetry 32 (1987), S. 1127-1137 
    Details
    ISSN: 1572-8943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Das thermische Verhalten von 6-Amino-5-formyluracil (HFU), 6-Amino-1-methyl-5-formyluracil (1-HFU), 6-Amino-3-methyl-5-formyluracil (3-HFU) und 6-Amino-1,3-dimethyl-5-formyluracil (HDFU) wird beschrieben. Nur HDFU enthält Kristallwasser. Dehydratisierungs- und Schmelzenthalpiewerte wurden aus den DSC-Kurven berechnet. Auch über das thermische Verhalten neuer, durch Reaktion der obigen Pyrimidin-Derivate mit Ni(II)-, Cu(II)- und Pd(II)-Ionen erhaltenen Komplexe wird berichtet.
    Abstract: Резюме Описано термическое поведение 6-амино-5-форм ил-, 6-амино-1-метил-5-формил-, 6а мино-З-метил-5-формили 6-амино-1,3-диметил-5-форми лурацилов. Показано, ч то только последнее соединени е содержит кристалли зационную воду. На основе кривых ДСК вычислены значения энтальпии д егидратации и плавле ния. Подобным образом изу чено также термическ ое разложение комплекс ов вышеприведенных п роизводных пиримидина с двухвал ентными никелем, медью и палла дием.
    Notes: Abstract The thermal behaviour of 6-amino-5-formyluracil (HFU), 6-amino-1-methyl-5-formyluracil (1-MFU), 6-amino-3-methyl-5-formyluracil (3-HFU) and 6-amino-1,3-dimethyl-5-formyluracil (HDFU) is described. Only HDFU is shown to contain crystallization water. Dehydration and fusion enthalpy values have been calculated from the DSC curves. Likewise, the thermal behaviour of new complexes obtained by reaction between the above pyrimidine derivatives and Ni(II), Cu(II) and Pd(II) ions is reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01905167
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  • 4
    Electronic Resource
    Electronic Resource
    Hydrogenation of CO2 on Fe/carbon catalysts (1986)
    Springer
    Reaction kinetics and catalysis letters 31 (1986), S. 349-354 
    Details
    ISSN: 1588-2837
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Abstract Гидрирование CO2 на железных катализаторах, нанесенных на активированный углерод, исследовано при атмосферном давлении и при соотношении H2/CO2=4. Специфическая активность для этой реакции ниже, чем в синтезе Фишера-Тропша на тех же самых катализаторах. CO и CH4 являются единственными продуктами, образующимися в гидрировании CO2 в данных экспериментальных условиях. Кажущаяся энергия активации обра-зования CO ниже, чем энергия активации образования CH4.
    Notes: Abstract The hydrogenation of CO2 on activated carbon-supported iron catalysts has been carried out at atmospheric pressure and using a H2/CO2 ratio of 4. The specific activities for this reaction are lower than those obtained for the Fischer-Tropsch synthesis using the same catalysts. CO and CH4 are the only products obtained in the hydrogenation of CO2 under the experimental conditions used. The apparent activation energies determined for the production of CO are lower than those for the production of CH4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02072969
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  • 5
    Electronic Resource
    Electronic Resource
    Determination of clotiazepam by HPLC with spectrophotometric and amperometric detection (1987)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 328 (1987), S. 117-119 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Spectrophotometric and amperometric detectors were compared for the determination of clotiazepam by HPLC. Methanol-water (60∶40) containing 0.01 mol/l ammonium acetate and methanol-water (70∶30) containing 0.01 mol/l acetate buffer pH 4 were used as mobile phase in the spectrophotometric and amperometric detection, respectively. The minimum detectability for the spectrophotometric detection was 20 ng injected and the method was applied for the determination of clotiazepam in serum, whereas for the amperometric detection a limit of detection of 2.5 νg injected was obtained, using this technique for clotizepam determination in tablets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00560964
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural immunocytochemical study of the massa caudalis of the subcommissural organ-Reissner's fiber complex in lamprey larvae (Geotria australis): Evidence for a terminal vascular route of secretory material (1987)
    Springer
    Cell & tissue research 247 (1987), S. 367-376 
    Details
    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Reissner's fiber ; Massa caudalis ; Vasculature ; Ultrastructural immunocytochemistry ; Lamprey ; Geotria australis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The massa caudalis of the subcommissural organ-Reissner's fiber complex of lamprey larvae (Geotria australis) was studied immunocytochemically at the ultrastructural level by use of the immunoperoxidase-silver methenamine procedure. An antiserum raised against bovine Reissner's fiber was utilized as primary antibody. The caudalmost portion of the central canal and its ampulla caudalis communicate, via wide intercellular spaces in their dorsal wall, with large cavities or lacunae. In addition, distinct openings in the dorsal wall of the ampulla establish an open communication between the latter and the lacunae. The lacunae are lined by slender processes of cells of unknown nature. No junctional complexes can be observed between these cells, which lack a basal lamina. The lacunae communicate with structures resembling blood capillaries, however, they are devoid of a basal lamina. These peculiar vessels, in turn, are in direct communication with characteristic blood capillaries. Reissner's fiber (RF) and its massa caudalis are strongly immunoreactive with the antiserum used. The wide intercellular spaces in the dorsal wall of the central canal and the ampulla, as well as the lumina of the (i) lacunae, (ii) modified vessels and (iii) blood capillaries are filled with a flocculent, strongly immunoreactive material. No immunoreactive material was found outside these structures. Thus, the blood capillaries appear to represent the only final target of RF-material arriving at the ampulla caudalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218318
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  • 7
    Electronic Resource
    Electronic Resource
    Reissner's fiber and the wall of the central canal in the lumbo-sacral region of the bovine spinal cord (1985)
    Springer
    Cell & tissue research 240 (1985), S. 649-662 
    Details
    ISSN: 1432-0878
    Keywords: Reissner's fiber ; Subcommissural organ ; Ependyma ; Immunocytochemistry ; Electron microscopy ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Reissner's fiber (RF) of the subcommissural organ (SCO), the central canal and its bordering structures, and the filum terminale were investigated in the bovine spinal cord by use of transmission electron microscopy, histochemical methods and light-microscopic immunocytochemistry. The primary antisera were raised against the bovine RF, or the SCO proper. Comparative immunocytochemical studies were also performed on the lumbo-sacral region of the rat, rabbit, dog and pig. At all levels of the bovine spinal cord, RF was strongly immunoreactive with both antisera. From cervical to upper sacral levels of the bovine spinal cord there was an increasing number of ependymal cells immunostainable with both antisera. The free surface of the central canal was covered by a layer of immunoreactive material. At sacral levels small subependymal immunoreactive cells were observed. From all these structures sharing the same immunoreactivity, only RF was stained by the paraldehyde-fuchsin and periodicacid-Schiff methods. At the ultrastructural level, ependymal cells with numerous protrusions extending into the central canal were seen in the lower lumbar segments, whereas cells displaying signs of secretory activity were principally found in the ependyma of the upper sacral levels. A few cerebrospinal fluid-contacting neurons were observed at all levels of the spinal cord; they were immunostained with an anti-tubulin serum. The lumbo-sacral segments of the dog, rat and rabbit, either fixed by vascular perfusion or in the same manner as the bovine material, did not show any immunoreactive structure other than RF. The possibilities that the immunoreactive ependymal cells might play a secretory or an absorptive role, or be the result of post-mortem events, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216353
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  • 8
    Electronic Resource
    Electronic Resource
    Light and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: Evidence for processing of the secretory material (1986)
    Springer
    Cell & tissue research 243 (1986), S. 545-559 
    Details
    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Glycoproteins ; Secretory process ; Immunocytochemistry ; Lectin histochemistry ; Rat ; Vertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcommissural organ (SCO) of the rat was investigated by use of histochemical and immunocytochemical methods at the light and electron-microscopic levels. Consecutive thin methacrylate sections were stained with the pseudoisocyanin (Psi), immunoperoxidase (IMC; employing an antiserum against Reissner's fiber, AFRU), periodic acid-Schiff (PAS) and periodic acid-silver methenamine (SM) techniques, and reacted with six types of lectins. Psi, SM, concanavalin A (Con A) and IMC were also used for double and triple sequential staining of the same section. Increasing dilutions of AFRU (from 1∶1000 to 1∶200 000) were used for immunostaining of serial paraffin sections. In addition, ultrastructural localization of (i) Con A-binding sites and (ii) immunoreactive secretory material was performed. Some of these procedures were also applied to the ophidian and canine SCO. Con A-positive, Psi-positive and immunoreactive materials coexisted within the same cisternae of the rough endoplasmic reticulum. The Golgi apparatus lacked Con A-positive and immunoreactive substances. Apical secretory granules and secreted material lying on the surface of the SCO showed (i) the highest affinity for AFRU, but were (ii) Con A-negative, and (iii) wheat-germ agglutinin-, PAS and SM-positive. Reissner's fiber displayed a low affinity for AFRU. It is suggested that the SCO secretes N-linked glycoproteins, the carbohydrate and protein moeities of which undergo (i) a maturation process before being released, and (ii) some kind of modification(s) after their release into the ventricle. The perivascular secretory cells of the dog SCO might secrete a material different from that secreted by the ependymal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218061
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  • 9
    Electronic Resource
    Electronic Resource
    Light- and electron-microscopic investigation of the rat subcommissural organ grafted under the kidney capsule, with particular reference to immunocytochemistry and lectin histochemistry (1989)
    Springer
    Cell & tissue research 258 (1989), S. 499-514 
    Details
    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Secretory activity, neural control ; Transplantation ; Long-spacing collagen ; Immunocytochemistry ; Molecular markers (neuronal, glial) ; Electron microscopy ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary There is increasing evidence that, in the rat, a serotonin-mediated neural input may have an inhibitory influence on the secretory activity of the subcommissural organ (SCO). In the present investigation the rat SCO was studied 7, 30 and 90 days after transplantation under the kidney capsule, an area devoid of local serotonin-containing nerves. The grafted tissue was examined by use of immunocytochemistry employing a series of primary antisera, lectin histochemistry and transmission electron microscopy. The grafted SCO survived transplantation and contained, in addition to secretory ependymal and hypendymal SCO-cells, also elements immunoreactive with antisera against glial fibrillary acidic protein or S-100 protein. In transplants, SCO-cells produced a material displaying the characteristic immunocytochemical and lectin-binding properties of SCO-cells observed under in-situ conditions. The ependymal cells lined 1–3 small cavities, which contained secretory material. A fully developed structural equivalent of Reissner's fiber was, however, never found. The immunocytochemical and ultrastructural study of the grafted SCO showed an absence of nerve fibers within the graft and suggested a state of enhanced secretory activity. A network of protruding basal lamina structures connected the secretory cells to the newly formed capillaries revascularizing the SCO. One week after transplantation, long-spacing collagen started to appear in expanded areas of such laminar networks and also in the perivascular space. It is suggested (i) that the formation of long-spacing forms of collagen is triggered by factors provided by the SCO-secretory cells, and (ii) that secretory material of the ependymal and hypendymal cells may reach the reticular extensions of the basal lamina. In contrast to the SCO in situ, the grafted SCO-cells showed a positive immunoreaction for neuron-specific enolase. They became surrounded by a S-100-immunoreactive glial sheath that separated them from other transplanted cell types and the adjacent kidney tissue of the host.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218862
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  • 10
    Electronic Resource
    Electronic Resource
    Comparison between isoelectric focusing methods for the detection of orosomucoid phenotypes (1988)
    Weinheim : Wiley-Blackwell
    Electrophoresis 9 (1988), S. 268-272 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Orosomucoid (ORM) polymorphism was investigated by different methods including isoelectric focusing in acid pH ranges followed by silver staining, print immunofixation of desialyzed ORM, fixation using a lectin from the sea-weed Codium tomentosum, isoelectric focusing followed by immunofixation in miniaturized gels and isoelectric focusing in immobilized pH gradients. Population genetics studies were carried out in Galicia (NW Spain) and two new ORM variants were found.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150090605
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