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  • 1
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    The Mark II detector for the SLC (1989)
    Elsevier
    Details
    Publication Date: 1989-08-01
    Print ISSN: 0168-9002
    Electronic ISSN: 1872-9576
    Topics: Physics
    Published by Elsevier
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Rotor electrometer: New instrument for bulk matter quark search experiments (1986)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 57 (1986), S. 2691-2698 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The rotor electrometer is a new instrument which we hope will make possible searches for rare fractionally charged impurities in very large quantities of matter. The ultimate goal of the project is to be able to measure the net charge of 10 mg samples of any material to an accuracy of 0.05 qe in a few minutes (qe is the electron's charge). This paper reports the achievement of subelectron (0.3 qe) charge resolution with the new device. We discuss effects which limit the resolution and consider prospects for improving the performance to the point where a fractional charge search may be attempted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139079
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  • 3
    Electronic Resource
    Electronic Resource
    Time domain dielectric measurements of conducting glasses (1986)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 84 (1986), S. 6518-6519 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Dielectric measurements of ionically conducting glasses in the family 0.2Li2O ⋅ yLiF ⋅ B2O3 with y=0,0.1,0.2,0.3,0.4,0.5,0.6, and 0.7, have been made in the 107–1010 Hz region by time domain reflectometry (TDR) at six temperatures in the 25–375 °C range. The presence of a plateau in Ε' in the ∼107–109 Hz region below an absorptive process near 109 Hz constitutes a static permittivity, Εs, whose temperature coefficient leads to the result that (∂S/∂E2)T is positive and, thus, that the system becomes more disordered upon application of the electric field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.450749
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  • 4
    Electronic Resource
    Electronic Resource
    ATP synthesis and utilization in the early stage of seed germination in relation to seed dormancy and quality (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 66 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In addition to the possible activities of the well-known biochemical cycles, the ATP-synthesizing system in dry seeds consists of a pathway which oxidizes malate and provides phosphoenolpyruvate (PEP). This system seems to be regulated primarily by the malate dehydrogenase (EC 1.1.1.37) activity, which together with the PEP carboxylase (EC 4.1.1.38) provides PEP and NADH. This latter compound may play a role as the “sparker” for the whole cycle.Breaking seed dormancy does not seem to be related to the rate of ATP synthesis per se but to facilitate enzyme-substrate interaction, probably caused by intracellular compartment-fusion. The compartmentation process is probably a result of the seed ripening and maturation functions.The ATP accumulated at the early stage of seed germination is a result of ATP synthesis and ATP utilization. Thus, ATP accumulation may result from a high rate of synthesis (high quality seeds) or from a low utilization ability (low-quality seeds). Also, since 95% of the synthesized ATP is utilized concomitantly with its synthesis, the accumulated amount is relatively insignificant and does not indicate seed quality. This conclusion is also supported by many experimental results in which no such correlation was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb01253.x
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  • 5
    Electronic Resource
    Electronic Resource
    The One-Charged Particle Decay Modes of the Tau (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 535 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb51543.x
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  • 6
    Electronic Resource
    Electronic Resource
    In situ hybridization using 32P labelled oligodeoxyribonucleotides for the cellular localisation of mRNA in neuronal and endocrine tissue (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 467-479 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Methodological variables for in situ hybridization using 32P labelled oligodeoxyribonucleotides (oligomers) have been examined. Four different oligomers directed against proglucagon messenger RNA (mRNA) and two different oligomers against prosomatostatin mRNA have been used. Specific hybridization was obtained in adult rat brain, stomach and pancreas and in neonatal rat ileum. Tissue was perfusion fixed with 4% paraformaldehyde 0.2% glutaraldehyde and hybridization was carried out in 50% formamide for 72 h at 42° C. Using hybridization conditions of lower stringency (33% formamide) labelling was also obtained in guinea pig tissue. Other variables which affected hybridization signal intensity were the inclusion of a prehybridization dehydration stage, the probe concentration, the inclusion of ammonium acetate in the posthybridization dehydrating ethanols and in the autoradiographic emulsion, and the exposure time. The localisation of proglucagon mRNA in rat pancreas using a 20mer was used as a model tissue for testing these methodological variables and the results were found generally also to apply to the other probes and tissues tested. The methods described provide single cell resolution and show that 32P labelled oligomers may be used to localise neuropeptide and endocrine mRNAs in different types of tissue and in different mammalian species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492604
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  • 7
    Electronic Resource
    Electronic Resource
    Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene (1988)
    Springer
    Plant cell reports 7 (1988), S. 403-406 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ethylene release by potato shoots cultured in closed boxes was suppressed by the addition of silver thiosulfate to the culture medium. Shoots cultured in the presence of silver thiosulfate produced appreciably more tissue and the yield of protoplasts per unit tissue mass was vastly increased, resulting in an 8 fold increase of protoplast yield per shoot. Exposure of pricked leaves to macerating enzymes facilitated ethylene generation. Leaves of shoots which were previously cultured in silver thiosulfate containing medium generated much less ethylene than leaves from control shoots and this generation could be further reduced by the addition of acetylsalicylic acid during maceration. The capability of polyethylene glycol treated potato protoplasts to produce microcalli was vastly increased by the addition of silver thiosulfate during exposure of protoplasts to Ca(NO3)2 following the polyethylene glycol treatment. Similarly, when a plasmid (pCAP212) containing an expressible gene for chloramphenicol acetyltransferase was introduced into potato protoplasts through a polyethylene glycol treatment, the transient expression of acetyltransferase was very much increased by the addition of a short incubation of the protoplasts with silver thiosulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269523
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  • 8
    Electronic Resource
    Electronic Resource
    The Cucumis plastome: physical map, intrageneric variation and phylogenetic relationships (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 417-429 
    Details
    ISSN: 1432-2242
    Keywords: Chloroplast DNA ; Cucumis ; Restriction-patterns ; Phylogeny ; Plastome ; Parsimonious tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A restriction map of the Cucumis melo L. (melon) plastome was constructed by using several mapping approaches: single and double digestions of the chloroplast DNA (chlDNA) with endonucleases (XhoI, SmaI, SacI and PvuII) and hybridization to heterologous chlDNA probes and to isolated melon chlDNA fragments. Four plastome-coded genes were located using heterologous probes. The overall organization and gene position of the melon plastome was found to be similar to that of tobacco and other angiosperm species. Restriction patterns based on digestion of the chlDNA with nine endonucleases were obtained in over 20 wild species and cultivated varieties of Cucumis. These led to mutational analysis of the restiction sites yielding the most parsimonious phylogenetic tree of the Cucumis plastome. Most African species from a compact group (“Anguria group”) which is distant from the melon, the cucumber and a few other species (C. sagittatus, C. metuliferus and C. humifructus). All of these are also far apart from each other. The distribution of polymorphic restriction sites along the Cucumis plastome is described and conservative regions as well as “hot spots” are suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251182
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  • 9
    Electronic Resource
    Electronic Resource
    Phylogeny of Cucumis based on isozyme variability and its comparison with plastome phylogeny (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 430-436 
    Details
    ISSN: 1432-2242
    Keywords: Isozymes ; Phylogeny ; Cucumis ; Chloroplast DNA ; Dendrogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An electrophoretic comparison of 29 nuclear-coded enzymes was carried out for 21 Cucumis species, and a phylogeny based on pairwise measurements of the respective genetic distances was computed. This phylogeny was compared to the one based on chlDNA cariation (Perl-Treves and Galun 1985). The two phylogenies were found to share the main dendrogram features; they also agree well with most taxonomic data available on Cucumis. Accordingly, most of the African Cucumis species form a close group (“Anguria group” — “Group A”), which is distant from the melon (C. melo), and from a few other distinct species, all of which are far apart from each other. The cucumber (C. sativus) is the most distant species within the genus. Some specific taxonomic implications as well as some general evolutionary problems related to such a parallel investigation of the nuclear genome and the plastome are evaluated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251183
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  • 10
    Electronic Resource
    Electronic Resource
    Contrasting effects of RNA and protein synthesis blocking on natural and lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1344-1346 
    Details
    ISSN: 1420-9071
    Keywords: Natural cell-mediated cytotoxicity ; lectin-dependent cell-mediated cytotoxicity ; RNA synthesis ; protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this study, earlier observations2,9 concerning the independence of both natural (NCMC) and lectin-dependent cell-mediated cytotoxicity (LDCC) from DNA synthesis have been confirmed. In addition, blocking of RNA synthesis by actinomycin D and of protein synthesis, reversibly by puromycin (PM) and irreversibly by emetine (EM) had different effects on NCMC and LDCC against3H-thymidine-prelabeled HEp-2 target cells. Similarly to the Con A-induced proliferation of lymphocytes, LDCC activity was also inhibited by blocking of RNA and protein synthesis. NCMC to HEp-2 target cells was not affected by blocking of RNA synthesis, while both PM and EM strongly enhanced NCMC activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952087
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