ISSN:
1573-5028
Keywords:
aleurone
;
cDNA library
;
differential screening
;
DNA sequence
;
tissue-specific expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The cloning of 11 different homology groups of cDNAs representing genes expressed in aleurone, but not in starchy endosperm of 20-day-old barley grains is described. Among the cDNAs, four are aleurone-specific, while the remaining are also expressed in the embryo, but not in any other part of the plant. Sequence analysis of one of the aleurone-specific clones, B11E, reveals an open reading frame coding for an unidentified 10.4 kDa protein with a putative signal sequence and a possible metal-binding finger. The B11E gene has a high GC content in the 5′ leader sequence (63%), as well as in the coding region (70%) compared to known cDNAs from the barley starchy endosperm. Northern analysis of B11E indicates maximum mRNA abundance around mid-phase of grain development. When isolated immature aleurone/pericarp is incubated in tissue culture medium (MS) the B11E message disappears, indicating a requirement for a diffusible factor from the intact grain for its continued presence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043205
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